ABSTRACT
The study was designed to evaluate the effects of the total alkaloid extract of Algerian Peganum harmala seeds on sexual behavior and male reproductive function. After two weeks of acclimatization, the male mice were randomly divided into four groups (seven mice in each group). For 35 days, the extract was administered orally at dose levels of 6.25, 12.5, and 25 mg/kg body weight per day to the respective groups of male mice (n = 7) and normal saline daily to the control group. On day 28, sexual behavior parameters were recorded. At the end of the trial, reproductive organ weights, sperm quality, seminal fructose, and testosterone hormone levels were evaluated. The three treated groups were compared with the control using statistical variance analysis (one-way ANOVA, p < 0.05), followed by Tukey's test. The results of the groups treated with 12.5 and 6.25 mg/kg of P. harmala alkaloid revealed the MF and IF parameters to be the lowest compared to the control group (p < 0.05). However, the male mice treated with 25 mg/kg recorded the highest values. A low significant value of ML was observed in the group treated with 25 mg/kg of the total alkaloid extract of P. harmala compared to the control group (p < 0.01), while a rise was observed in the concentration group treated with 6.25 mg/kg. Regarding IL, the male mice treated with different concentrations of the total alkaloid extract of P. harmala recorded a higher time than the control group. Moreover, an increase in the gonadosomatic index was noticed in all groups compared to the control group. However, there was a significant (p < 0.01) decrease in the sperm counts of the groups treated with 12.5 mg/kg and 6.25 mg/kg. However, there was no significant difference in the motility, membrane integrity, and total antioxidant capacity of sperm cells compared to the control. The extract treatment also brought about a non-significant increase in fructose content of the seminal vesicle and serum testosterone level. The findings of this study demonstrate that the extract acts in a dose-dependent manner, and it has varying effects on the reproductive parameters of male mice.
ABSTRACT
This epidemiological study aimed to determine the species of tick infestation in dogs, their prevalence and dynamic in the Bejaia province, northeastern Algeria. A total of 631 dogs were examined from different localities of the Bejaia province between March 2016 and February 2017. Of the 631 examined dogs, 15% were infested with one or more tick species. A total of 339 adult ticks were collected and identified, including 199 male tick species and 140 female tick species. Our results revealed that most of these were Rhipicephalus species, with Rhipicephalus sanguineus (51.32%) being the most prevalent followed by Rhipicephalus bursa (35.1%) and Rhipicephalus turanicus (12.98%). Ixodes ricinus represented only 0.6% of all ticks collected. The highest infested seasons were spring (22.55%) and summer (22.54%) and the lowest infested seasons were autumn (8.62%) and winter ( 0.9%). There is no significant difference between the sex of the animal and the prevalence of infestation (p = 0.837). Also, the prevalence of infestation by ticks in young animals was higher than that in adult animals (p = 0.550). A significant difference between the prevalence of infestation and animal breed was observed (p = 0.042). This study is the first epidemiological investigation conducted on the prevalence of hard ticks infesting domestic dogs in Bejaia (northeastern Algeria) based on conventional methods. It is therefore necessary to implement an effective tick control strategy during infestation periods in order to prevent vector-borne diseases.
