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1.
Am J Perinatol ; 33(14): 1357-1364, 2016 12.
Article in English | MEDLINE | ID: mdl-27135955

ABSTRACT

Objective The objective of this study was to localize by neuroimaging the altered structural brain development of these offspring using an autism model of transgenic mice lacking contactin-associated protein-like 2 (Cntnap2). Materials and Methods Pregnant dams were randomly allocated to fructose solution (10% W/V) as only drinking fluid or water. Cntnap2 heterozygous (+/-) offspring from each group were euthanized at 6 months of age and their whole brains evaluated by magnetic resonance imaging. T2-weighted images were acquired to evaluate the volumes of 29 regions of interest involved in autism spectrum disorder (ASD) pathogenesis. Whole brains were washed and processed for Nissl staining. Mann-Whitney U test and one-way analysis of variance were used for statistical analysis (significance: p < 0.05). Results The corpus callosum, anterior commissure, and caudate putamen were significantly smaller in Cntnap2 (+/-) male offspring exposed to fructose. No brain alterations were found in the female counterparts. Nissl staining of the caudate putamen revealed higher neuronal cell count in the male fructose offspring. Female group revealed an increase in caudate putamen neuronal cell count. Conclusion Metabolic dysregulation in pregnancy alters fetal brain development in genetically predisposed offspring. This is consistent with findings in human studies and supports the role of intrauterine factors in the etiology of autism.


Subject(s)
Autism Spectrum Disorder/diagnostic imaging , Autism Spectrum Disorder/pathology , Brain/embryology , Brain/pathology , Fructose/administration & dosage , Maternal Nutritional Physiological Phenomena , Animals , Autism Spectrum Disorder/genetics , Disease Models, Animal , Female , Fetal Development , Magnetic Resonance Imaging , Male , Membrane Proteins/genetics , Mice , Mice, Inbred C57BL , Mice, Transgenic , Nerve Tissue Proteins/genetics , Pregnancy , Random Allocation , Sex Factors
2.
Reprod Sci ; 23(11): 1593-1599, 2016 11.
Article in English | MEDLINE | ID: mdl-27170663

ABSTRACT

OBJECTIVE: Using an animal model of preeclampsia induced by overexpression of soluble fms-like tyrosine kinase 1 (sFlt-1), we previously showed that pravastatin prevents the development of a preeclampsia phenotype. Our objective is to determine whether pravastatin treatment may be explained by its effects on apoptotic/survival pathways in the placenta. METHODS: Pregnant CD1 mice at day 8 of gestation (length of gestation 19 days) were randomly allocated to injection via tail vein with either adenovirus carrying sFlt-1 or adenovirus carrying the murine immunoglobulin G2α Fc fragment (mFc virus control group). Mice from the sFlt group were randomly assigned to receive pravastatin (5 mg/kg/d) in their drinking water from day 9 until killing (sFlt-1 + Pravastatin) or water (sFlt-1). The mFc control received water only. Mice were killed on day 18, and the placentas were collected. Protein mitogen-activated protein kinase (MAPK) pathway substrates were assayed using Bioplex Multiplex Immunoassay (Bio-Rad, Hercules, California). Data are reported as mean ± standard error of the mean or median (interquartile range) when appropriate. One-way analysis of variance followed by post hoc analysis was performed. Two-sided P value < .05 was considered statistically significant. RESULTS: The sFlt-1 + Pravastatin mice had significantly higher placental protein concentrations of prosurvival/ antiapoptotic factors (activating transcription factor 2, pp38, phosphorylated c-jun N-terminal kinase, and phosphorylated extracellular signal-regulated kinase) and of heat-shock protein 27 and signal transducer and activator of transcription 3, 2 factors crucial for embryonic and placental development during oxidative stress, compared to sFlt-1 mice (P < .05) and similar to the mFc control group. No differences were noted in substrates of the proapoptotic pp53 pathway. CONCLUSION: Pravastatin ability to prevent preeclampsia phenotype may be mediated through pleiotropic mechanisms involving a prosurvival/ antiapoptotic MAPK pathway in the placenta. Our results further support continued research in the role for statins in the prevention of preeclampsia.


Subject(s)
Apoptosis/drug effects , Placenta/drug effects , Placenta/metabolism , Pravastatin/administration & dosage , Pre-Eclampsia/metabolism , Animals , Disease Models, Animal , Female , HSP27 Heat-Shock Proteins/metabolism , MAP Kinase Signaling System/drug effects , Mice , Phosphorylation , Pre-Eclampsia/prevention & control , Pregnancy , STAT3 Transcription Factor/metabolism , Vascular Endothelial Growth Factor Receptor-1/metabolism
3.
Am J Obstet Gynecol ; 215(3): 378.e1-6, 2016 09.
Article in English | MEDLINE | ID: mdl-27060421

ABSTRACT

BACKGROUND: Consumption of fructose-rich diets in the United States is on the rise and thought to be associated with obesity and cardiometabolic diseases. OBJECTIVE: We sought to determine the effects of antenatal exposure to high-fructose diet on offspring's development of metabolic syndrome-like phenotype and other cardiovascular disease risk factors later in life. STUDY DESIGN: Pregnant C57BL/6J dams were randomly allocated to fructose solution (10% wt/vol, n = 10) or water (n = 10) as the only drinking fluid from day 1 of pregnancy until delivery. After weaning, pups were started on regular chow, and evaluated at 1 year of life. We measured percent visceral adipose tissue and liver fat infiltrates using computed tomography, and blood pressure using CODA nonivasive monitor. Intraperitoneal glucose tolerance testing with corresponding insulin concentrations were obtained. Serum concentrations of glucose, insulin, triglycerides, total cholesterol, leptin, and adiponectin were measured in duplicate using standardized assays. Fasting homeostatic model assessment was also calculated to assess insulin resistance. P values <.05 were considered statistically significant. RESULTS: Maternal weight, pup number, and average weight at birth were similar between the 2 groups. Male and female fructose group offspring had higher peak glucose and area under the intraperitoneal glucose tolerance testing curve compared with control, and higher mean arterial pressure compared to control. Female fructose group offspring were heavier and had higher percent visceral adipose tissue, liver fat infiltrates, homeostatic model assessment of insulin resistance scores, insulin area under the intraperitoneal glucose tolerance testing curve, and serum concentrations of leptin, and lower concentrations of adiponectin compared to female control offspring. No significant differences in these parameters were noted in male offspring. Serum concentrations of triglycerides or total cholesterol were not different between the 2 groups for either gender. CONCLUSION: Maternal intake of high fructose leads to fetal programming of adult obesity, hypertension, and metabolic dysfunction, all risk factors for cardiovascular disease. This fetal programming is more pronounced in female offspring. Limiting intake of high fructose-enriched diets in pregnancy may have significant impact on long-term health.


Subject(s)
Diet , Fructose/administration & dosage , Hypertension/etiology , Insulin Resistance , Obesity/etiology , Prenatal Exposure Delayed Effects , Animals , Blood Glucose/analysis , Fatty Liver/etiology , Female , Fructose/adverse effects , Glucose Tolerance Test , Intra-Abdominal Fat/anatomy & histology , Leptin/blood , Mice, Inbred C57BL , Pregnancy
4.
Infect Immun ; 75(10): 4792-8, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17664261

ABSTRACT

In previous studies we showed that a Cryptococcus neoformans mutant lacking glucosylceramide (Deltagcs1) is avirulent and unable to reach the brain when it is administered intranasally into an immunocompetent mouse and is contained in a lung granuloma. To determine whether granuloma formation is key for containment of C. neoformans Deltagcs1, we studied the role of C. neoformans glucosylceramide in a T- and NK-cell-immunodeficient mouse model (Tgepsilon26) in which alveolar macrophages (AMs) are not activated and granuloma formation is not expected. The results show that Tgepsilon26 mice infected with Deltagcs1 do not produce a lung granuloma and that the Deltagcs1 mutant proliferates in the lungs and does disseminate to the brain, although its virulence phenotype is dramatically reduced. Since Deltagcs1 can grow only in acidic niches, such as the phagolysosome of AMs, and not in neutral or alkaline environments, such as the extracellular spaces, we hypothesize that in immunodeficient mice Deltagcs1 proliferates inside AMs. Indeed, we found that depletion of AMs significantly improved Tgepsilon26 mouse survival and decreased the dissemination of Deltagcs1 cells to the central nervous system. Thus, these results suggest that the growth of Deltagcs1 in immunodeficient mice is maintained within AMs. This study highlights the hypothesis that AMs may exacerbate C. neoformans infection in conditions in which there is severe host immunodeficiency.


Subject(s)
Cryptococcosis/immunology , Cryptococcus neoformans/pathogenicity , Glucosylceramides/biosynthesis , Lung/immunology , Macrophages, Alveolar/immunology , Virulence Factors/biosynthesis , Animals , Brain/microbiology , Colony Count, Microbial , Cryptococcosis/microbiology , Cryptococcus neoformans/genetics , Glucosylceramides/genetics , Glucosylceramides/immunology , Granuloma/pathology , Immunocompromised Host , Kidney/microbiology , Lung/microbiology , Lung/pathology , Mice , Mice, Mutant Strains , Mice, Transgenic , Spleen/microbiology , Survival Analysis , Virulence Factors/immunology
5.
Infect Immun ; 74(10): 5977-88, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16988277

ABSTRACT

In recent years, sphingolipids have emerged as critical molecules in the regulation of microbial pathogenesis. In fungi, the synthesis of complex sphingolipids is important for the regulation of pathogenicity, but the role of sphingolipid degradation in fungal virulence is not known. Here, we isolated and characterized the inositol phosphosphingolipid-phospholipase C1 (ISC1) gene from the fungal pathogen Cryptococcus neoformans and showed that it encodes an enzyme that metabolizes fungal inositol sphingolipids. Isc1 protects C. neoformans from acidic, oxidative, and nitrosative stresses, which are encountered by the fungus in the phagolysosomes of activated macrophages, through a Pma1-dependent mechanism(s). In an immunocompetent mouse model, the C. neoformans Deltaisc1 mutant strain is almost exclusively found extracellularly and in a hyperencapsulated form, and its dissemination to the brain is remarkably reduced compared to that of control strains. Interestingly, the dissemination of the C. neoformans Deltaisc1 strain to the brain is promptly restored in these mice when alveolar macrophages are pharmacologically depleted or when infecting an immunodeficient mouse in which macrophages are not efficiently activated. These studies suggest that Isc1 plays a key role in protecting C. neoformans from the intracellular environment of macrophages, whose activation is important for preventing fungal dissemination of the Deltaisc1 strain to the central nervous system and the development of meningoencephalitis.


Subject(s)
Brain/microbiology , Cryptococcus neoformans/pathogenicity , Macrophages, Alveolar/microbiology , Meningitis, Cryptococcal/immunology , Meningitis, Cryptococcal/microbiology , Type C Phospholipases/physiology , Amino Acid Sequence , Animals , Brain/immunology , Cryptococcus neoformans/enzymology , Cryptococcus neoformans/genetics , Female , Gene Deletion , Lung/microbiology , Lung/pathology , Macrophages, Alveolar/immunology , Macrophages, Alveolar/ultrastructure , Mice , Mice, Inbred CBA , Molecular Sequence Data , Phagosomes/immunology , Phosphatidylinositols/metabolism , Sphingolipids/metabolism , Type C Phospholipases/genetics
6.
J Clin Invest ; 116(6): 1651-9, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16741577

ABSTRACT

The pathogenic fungus Cryptococcus neoformans infects humans upon inhalation and causes the most common fungal meningoencephalitis in immunocompromised subjects worldwide. In the host, C. neoformans is found both intracellularly and extracellularly, but how these two components contribute to the development of the disease is largely unknown. Here we show that the glycosphingolipid glucosylceramide (GlcCer), which is present in C. neoformans, was essential for fungal growth in host extracellular environments, such as in alveolar spaces and in the bloodstream, which are characterized by a neutral/alkaline pH, but not in the host intracellular environment, such as in the phagolysosome of macrophages, which is characteristically acidic. Indeed, a C. neoformans mutant strain lacking GlcCer did not grow in vitro at a neutral/alkaline pH, yet it had no growth defect at an acidic pH. The mechanism by which GlcCer regulates alkali tolerance was by allowing the transition of C. neoformans through the cell cycle. This study establishes C. neoformans GlcCer as a key virulence factor of cryptococcal pathogenicity, with important implications for future development of new antifungal strategies.


Subject(s)
Cryptococcus neoformans/enzymology , Cryptococcus neoformans/pathogenicity , Fungal Proteins/metabolism , Glucosyltransferases/metabolism , Virulence Factors/metabolism , Animals , Cell Cycle/physiology , Cryptococcus neoformans/cytology , Cryptococcus neoformans/genetics , Fungal Proteins/genetics , Glucosyltransferases/genetics , Humans , Hydrogen-Ion Concentration , Lung/metabolism , Lung/microbiology , Lung/pathology , Mice , Mice, Inbred CBA , Survival Rate , Virulence Factors/genetics
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