ABSTRACT
Tumor angiogenesis, which is an important step in the development of cancer, is directly regulated by vascular endothelial growth factor receptor 2 (VEGFR-2). In this study, we examined the association of five potentially functional VEGFR-2 polymorphisms with glioma risk in a Chinese Han population. Three SNPs, rs2071559, rs7667298 and rs2305948, showed a statistically significant increased association with the risk of glioma (P = 0.006, 0.005, and 0.012, respectively). Both haplotype and diplotype analyses consistently revealed that subjects carrying two copies of the haplotype "CGT" had a 42% reduced glioma risk compared with their respective noncarriers. Our findings suggested that VEGFR-2 gene variants might contribute to glioma susceptibility.
Subject(s)
Brain Neoplasms/ethnology , Brain Neoplasms/genetics , Genetic Variation/genetics , Glioma/ethnology , Glioma/genetics , Vascular Endothelial Growth Factor Receptor-2/genetics , Adolescent , Adult , Asian People/genetics , Case-Control Studies , Child , China/epidemiology , Female , Genetic Predisposition to Disease/ethnology , Genetic Predisposition to Disease/genetics , Haplotypes , Humans , Male , Middle Aged , Polymorphism, Single Nucleotide/genetics , Risk Factors , Young AdultABSTRACT
Objective:To investigate the possible association between Thr241Met polymorphism in the DNA repair gene X-ray repair cross-complementing group 3 (XRCC3) with genetic susceptibility to glioma in a Chinese Han population living in Shanghai and the surrounding provinces in east China. Methods: Genotyping by a TaqMan assay was performed in 771 brain glioma patients living in Shanghai and the surrounding provinces (Jiangsu.Zhejiang, Anhui.etc. )and in 752 control participants matched in age and gender. The genotyping results of TaqMan assay and the association between Thr241Met polymorphism in the DNA repair gene XRCC3 with genetic susceptibility to glioma were statistically analyzed. Results: Genotypes of 1 468 subjects (760 with brain glioma and 708 were cancer-free control) were successfully performed by TaqMan assay, with the successful rate being 96.4%. Statistical analysis result showed that gene(C/T) and genotype(C/CT/CT/T) frequencies of XRCC3 were not significantly different between the glioma and cancer-free groups. Compared with the CC genotype, the variant TC(P = 0. 909; adjusted by age and gender OR = 0. 981; 95%CI = 0. 701-1. 371) or TT(P=0. 642; adjusted by age and gender OR = 0. 7; 95%CI = 0. 156-3. 146) genotypes of XRCC3 Thr241Met were associated with a non-statistically significant increase of glioma risk. Conclusion: The variant TC or TT genotypes of XRCC3 Thr241Met may not be risk factors for brain glioma in Chinese Han population living in Shanghai and the surrounding provinces in east China.
