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Methods Mol Biol ; 2316: 89-96, 2022.
Article in English | MEDLINE | ID: mdl-34845688

ABSTRACT

A simplified dot-blot hybridization protocol for Potato spindle tuber viroid (PSTVd) detection in Solanaceae species is described here. The protocol uses an RNA DIG-labeled probe and a simplified extraction procedure that avoids the use of hazardous chemicals. PSTVd was detected in composite tomato leaf samples in a ratio of up to 1:15 of PSTVd-infected to non-infected tissue and in composite potato tuber samples in a ratio up to 1:5 of PSTVd-infected to non-infected tissue. In Brugmansia spp., PSTVd was detected solely in the standard sample extract preparation. The method is suitable for a reliable, large-scale sample screening especially where cost is a limiting factor.


Subject(s)
Solanum tuberosum , Viroids , Solanum lycopersicum , Nucleic Acid Hybridization , Plant Diseases , RNA Probes , RNA, Viral/genetics , Viroids/genetics
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