Isolation of a wild-type virulent phage of Helicobacter pylori and its simulated treatments of gastrointestinal Hp in vitro / 南方医科大学学报

<p><b>OBJECTIVE</b>To isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assisted penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp.</p><p><b>METHODS</b>The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment.</p><p><b>RESULTS</b>After a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not.</p><p><b>CONCLUSION</b>The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.</p>

Transforming of the drug resistance plasmid from Staphylococcus aureus into Escherichia coli / 南方医科大学学报

<p><b>OBJECTIVE</b>To discuss the possible mechanism of drug resistance transmission between Staphylococcus and Escherichia coli.</p><p><b>METHODS</b>The chloramphenicol resistance plasmid of Staphylococcus aureus was extracted to transform the sensitive Escherichia coli, and the drug-resistant Escherichia coli were screened by drug sensitivity test.</p><p><b>RESULTS</b>The drug-resistant Escherichia coli were successfully obtained.</p><p><b>CONCLUSION</b>Staphylococcus may have a natural shuttle plasmid of drug resistance, which can transform Escherichia coli under specific conditions.</p>

Surgical management of minor salivary gland tumors / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the clinical features of minor salivary gland tumors and to discuss the treatment modalities for these tumors.@*METHOD@#Retrospective analysis of 54 cases with minor salivary gland tumor operated in our hospital from 1997 to 2004.@*RESULT@#Among 54 cases with minor salivary gland tumors in this series, 16 patients lost of follow up. Among the remaining 38 patients, 2 patients with nasal cavity adenoid cystic carcinoma died of tumor recurrence 2 and 3 years after the surgery respectively, one patient with laryngeal myoepithelial carcinoma died of tumor recurrence 3 years after the surgery and one patient with paranasal sinus mucoepidermoid carcinoma died of recurrence 17 months after the surgery. Two patients with paranasal sinus adenoid cystic carcinoma recurred after the primary surgery and were survived without tumor after salvage surgery. The other patients survived with no tumor recurrence.@*CONCLUSION@#While different histopathology of minor salivary gland tumors were found in this group, malignant tumors were predominant, accounting for 81.4%. The choice of treatment for minor salivary gland tumors depends upon the location and the histopathology of the tumors. The treatment policy for benign tumors is simple tumor excision, while that for malignant tumors is surgery combined with pre- or post-operative radiation therapy. Complete surgical resection of tumor masses and tumor free margin is essential for successful treatment of malignant minor salivary gland tumors.