1.
J Microbiol Methods
; 60(1): 41-6, 2005 Jan.
Article
in English
| MEDLINE
| ID: mdl-15567223
ABSTRACT
3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.