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1.
ACS Biomater Sci Eng ; 6(3): 1449-1461, 2020 03 09.
Article in English | MEDLINE | ID: mdl-33455378

ABSTRACT

The brain machine interface (BMI) describes a group of technologies capable of communicating with excitable nervous tissue within the central nervous system (CNS). BMIs have seen major advances in recent years, but these advances have been impeded because of a temporal deterioration in the signal to noise ratio of recording electrodes following insertion into the CNS. This deterioration has been attributed to an intrinsic host tissue response, namely, reactive gliosis, which involves a complex series of immune mediators, resulting in implant encapsulation via the synthesis of pro-inflammatory signaling molecules and the recruitment of glial cells. There is a clinical need to reduce tissue encapsulation in situ and improve long-term neuroelectrode functionality. Physical modification of the electrode surface at the nanoscale could satisfy these requirements by integrating electrochemical and topographical signals to modulate neural cell behavior. In this study, commercially available platinum iridium (Pt/Ir) microelectrode probes were nanotopographically functionalized using femto/picosecond laser processing to generate laser-induced periodic surface structures (LIPSS). Three different topographies and their physical properties were assessed by scanning electron microscopy and atomic force microscopy. The electrochemical properties of these interfaces were investigated using electrochemical impedance spectroscopy and cyclic voltammetry. The in vitro response of mixed cortical cultures (embryonic rat E14/E17) was subsequently assessed by confocal microscopy, ELISA, and multiplex protein array analysis. Overall LIPSS features improved the electrochemical properties of the electrodes, promoted cell alignment, and modulated the expression of multiple ion channels involved in key neuronal functions.


Subject(s)
Astrocytes , Neuroglia , Animals , Iridium , Lasers , Microelectrodes , Rats
2.
Nanomedicine (Lond) ; 11(19): 2547-63, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27618972

ABSTRACT

AIM: Medium chain length-polyhydroxyalkanoate/multi-walled carbon nanotube (MWCNTs) nanocomposites with a range of mechanical and electrochemical properties were fabricated via assisted dispersion and solvent casting, and their suitability as neural interface biomaterials was investigated. MATERIALS & METHODS: Mechanical and electrical properties of medium chain length-polyhydroxyalkanoate/MWCNTs nanocomposite films were evaluated by tensile test and electrical impedance spectroscopy, respectively. Primary rat mesencephalic cells were seeded on the composites and quantitative immunostaining of relevant neural biomarkers, and electrical stimulation studies were performed. RESULTS: Incorporation of MWCNTs to the polymeric matrix modulated the mechanical and electrical properties of resulting composites, and promoted differential cell viability, morphology and function as a function of MWCNT concentration. CONCLUSION: This study demonstrates the feasibility of a green thermoplastic MWCNTs nanocomposite for potential use in neural interfacing applications.


Subject(s)
Elastomers/chemistry , Nanocomposites/chemistry , Nanotubes, Carbon/chemistry , Neurons/physiology , Polyhydroxyalkanoates/chemistry , Animals , Biocompatible Materials , Calcium/chemistry , Calcium/metabolism , Cell Adhesion , Cell Culture Techniques , Cell Survival , Electric Conductivity , Electric Stimulation , Feasibility Studies , Humans , Mechanical Phenomena , Mesencephalon/cytology , Particle Size , Rats, Sprague-Dawley , Surface Properties , Tissue Engineering
3.
Drug Discov Today ; 19(1): 88-94, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23962478

ABSTRACT

During a single decade of research, evidence has emerged that glial scar formation around the electro-tissue interface drives neural loss and increases the signal impedance of the electrodes, compromising the efficiency of the stimulating systems. Studies with conducting polymers (CPs) as electrode coatings have shown enhanced tissue integration and electrode performance in situ through biochemical and physicomechanical functionalisation. In this review, recent findings on CP modifications are provided in the context of neurospecific biomaterials, shedding light on the valuable impact of multifunctionalised strategies for biomedical applications.


Subject(s)
Biocompatible Materials/chemistry , Cicatrix/prevention & control , Electrodes, Implanted , Neuroglia/physiology , Polymers/chemistry , Animals , Biocompatible Materials/administration & dosage , Cicatrix/pathology , Humans , Neuroglia/pathology , Polymers/administration & dosage
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