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1.
Dalton Trans ; 53(28): 11867-11875, 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-38952206

ABSTRACT

Antibiotic resistance is a significant global concern, necessitating the development of either new antibiotics or advanced delivery methods. With this in mind, we report on the synthesis and characterisation of a new family of Metal-Organic Frameworks (MOFs), OnG6 MOFs, designed to act as multi-drug carriers for bacterial infection treatment. OnG6 is based on the pro-drug 4,4'-azodisalicylic acid (AZDH4), which in vivo produces two equivalents of para-aminosalicylic acid (ASA), a crucial drug for M. tuberculosis treatment. X-ray and computational studies revealed that OnG6 MOFs are mesoporous MOFs with etb topology and an [M2(AZD)] formula (M = Zn, OnG6-Zn; Mg, OnG6-Mg; Cu, OnG6-Cu; and Co, OnG6-Co), featuring 1-dimensional channel type pores of 25 Å diameter. OnG6 MOFs are the first reported MOFs bearing the ligand AZDH4, joining the family of mesoporous MOFs arranged in a honeycomb pattern. They absorb isoniazid (INH) and ciprofloxacin (CIPRO) with the former being a specific antibiotic for M. tuberculosis, and the latter being a broader-spectrum antibiotic. The stability of the MOFs and their capacity for antibiotic uptake depend on the nature of the metal ion, with OnG6-Mg demonstrating the highest drug absorption. The antimicrobial activity of these species was assessed against S. aureus and E. coli, revealing that the carriers containing CIPRO displayed optimal efficacy.


Subject(s)
Drug Carriers , Metal-Organic Frameworks , Metal-Organic Frameworks/chemistry , Metal-Organic Frameworks/pharmacology , Metal-Organic Frameworks/chemical synthesis , Drug Carriers/chemistry , Drug Carriers/chemical synthesis , Microbial Sensitivity Tests , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/chemical synthesis , Ciprofloxacin/pharmacology , Ciprofloxacin/chemistry , Isoniazid/chemistry , Isoniazid/pharmacology , Escherichia coli/drug effects , Mycobacterium tuberculosis/drug effects , Models, Molecular
2.
J Med Chem ; 67(4): 2321-2336, 2024 Feb 22.
Article in English | MEDLINE | ID: mdl-38300987

ABSTRACT

Bruton's tyrosine kinase (BTK), a member of the TEC family of kinases, is an essential effector of B-cell receptor (BCR) signaling. Chronic activation of BTK-mediated BCR signaling is a hallmark of many hematological malignancies, which makes it an attractive therapeutic target. Pharmacological inhibition of BTK enzymatic function is now a well-proven strategy for the treatment of patients with these malignancies. We report the discovery and characterization of NX-2127, a BTK degrader with concomitant immunomodulatory activity. By design, NX-2127 mediates the degradation of transcription factors IKZF1 and IKZF3 through molecular glue interactions with the cereblon E3 ubiquitin ligase complex. NX-2127 degrades common BTK resistance mutants, including BTKC481S. NX-2127 is orally bioavailable, exhibits in vivo degradation across species, and demonstrates efficacy in preclinical oncology models. NX-2127 has advanced into first-in-human clinical trials and achieves deep and sustained degradation of BTK following daily oral dosing at 100 mg.


Subject(s)
Protein Kinase Inhibitors , Protein-Tyrosine Kinases , Humans , Agammaglobulinaemia Tyrosine Kinase , Protein Kinase Inhibitors/adverse effects , Signal Transduction
3.
Blood ; 141(13): 1584-1596, 2023 03 30.
Article in English | MEDLINE | ID: mdl-36375120

ABSTRACT

Bruton tyrosine kinase (BTK) is essential for B-cell receptor (BCR) signaling, a driver of chronic lymphocytic leukemia (CLL). Covalent inhibitors bind C481 in the active site of BTK and have become a preferred CLL therapy. Disease progression on covalent BTK inhibitors is commonly associated with C481 mutations. Here, we investigated a targeted protein degrader, NRX-0492, that links a noncovalent BTK-binding domain to cereblon, an adaptor protein of the E3 ubiquitin ligase complex. NRX-0492 selectively catalyzes ubiquitylation and proteasomal degradation of BTK. In primary CLL cells, NRX-0492 induced rapid and sustained degradation of both wild-type and C481 mutant BTK at half maximal degradation concentration (DC50) of ≤0.2 nM and DC90 of ≤0.5 nM, respectively. Sustained degrader activity was maintained for at least 24 hours after washout and was equally observed in high-risk (deletion 17p) and standard-risk (deletion 13q only) CLL subtypes. In in vitro testing against treatment-naïve CLL samples, NRX-0492 was as effective as ibrutinib at inhibiting BCR-mediated signaling, transcriptional programs, and chemokine secretion. In patient-derived xenografts, orally administered NRX-0492 induced BTK degradation and inhibited activation and proliferation of CLL cells in blood and spleen and remained efficacious against primary C481S mutant CLL cells collected from a patient progressing on ibrutinib. Oral bioavailability, >90% degradation of BTK at subnanomolar concentrations, and sustained pharmacodynamic effects after drug clearance make this class of targeted protein degraders uniquely suitable for clinical translation, in particular as a strategy to overcome BTK inhibitor resistance. Clinical studies testing this approach have been initiated (NCT04830137, NCT05131022).


Subject(s)
Leukemia, Lymphocytic, Chronic, B-Cell , Humans , Agammaglobulinaemia Tyrosine Kinase , Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy , Leukemia, Lymphocytic, Chronic, B-Cell/genetics , Leukemia, Lymphocytic, Chronic, B-Cell/metabolism , Heterografts , Drug Resistance, Neoplasm , Protein Kinase Inhibitors/pharmacology , Protein Kinase Inhibitors/therapeutic use , Pyrimidines/therapeutic use
4.
Proc Natl Acad Sci U S A ; 113(6): 1540-5, 2016 Feb 09.
Article in English | MEDLINE | ID: mdl-26811472

ABSTRACT

Most metazoan E3 ligases contain a signature RING domain that promotes the transfer of ubiquitin from the active site of E2 conjugating enzymes to lysine residues in substrates. Although these RING-E3s depend on E2 enzymes for catalysis, how they turn on their E2s at the right time and place remains poorly understood. Here we report a phosphorylation-dependent mechanism that ensures timely activation of the E2 Ube2S by its RING-E3, the anaphase-promoting complex (APC/C); while phosphorylation of a specific serine residue in the APC/C coactivator Cdc20 prevents delivery of Ube2S to the APC/C, removal of this mark by PP2A(B56) allows Ube2S to bind the APC/C and catalyze ubiquitin chain elongation. PP2A(B56) also stabilizes kinetochore-microtubule attachments to shut off the spindle checkpoint, suggesting that cells regulate the E2-E3 interplay to coordinate ubiquitination with critical events during cell division.


Subject(s)
Anaphase-Promoting Complex-Cyclosome/metabolism , Ubiquitin/metabolism , Biocatalysis , Cdc20 Proteins/metabolism , HeLa Cells , Humans , Kinetochores/metabolism , Phosphorylation , Protein Binding , Protein Phosphatase 2/metabolism , Serine/metabolism
5.
Mol Cell Biol ; 35(23): 3962-73, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26370512

ABSTRACT

The c-Jun amino-terminal kinase (JNK) plays a role in inflammation, proliferation, apoptosis, and cell adhesion and cell migration by phosphorylating paxillin and ß-catenin. JNK phosphorylation downstream of AMP-activated protein kinase (AMPK) activation is required for high CO2 (hypercapnia)-induced Na,K-ATPase endocytosis in alveolar epithelial cells. Here, we provide evidence that during hypercapnia, JNK promotes the phosphorylation of LMO7b, a scaffolding protein, in vitro and in intact cells. LMO7b phosphorylation was blocked by exposing the cells to the JNK inhibitor SP600125 and by infecting cells with dominant-negative JNK or AMPK adenovirus. The knockdown of the endogenous LMO7b or overexpression of mutated LMO7b with alanine substitutions of five potential JNK phosphorylation sites (LMO7b-5SA) or only Ser-1295 rescued both LMO7b phosphorylation and the hypercapnia-induced Na,K-ATPase endocytosis. Moreover, high CO2 promoted the colocalization and interaction of LMO7b and the Na,K-ATPase α1 subunit at the plasma membrane, which were prevented by SP600125 or by transfecting cells with LMO7b-5SA. Collectively, our data suggest that hypercapnia leads to JNK-induced LMO7b phosphorylation at Ser-1295, which facilitates the interaction of LMO7b with Na,K-ATPase at the plasma membrane promoting the endocytosis of Na,K-ATPase in alveolar epithelial cells.


Subject(s)
Endocytosis , Homeodomain Proteins/metabolism , Hypercapnia/metabolism , JNK Mitogen-Activated Protein Kinases/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Transcription Factors/metabolism , Amino Acid Sequence , Animals , Carbon Dioxide/metabolism , Cell Line , Enzyme Activation , Homeodomain Proteins/analysis , Homeodomain Proteins/genetics , Humans , JNK Mitogen-Activated Protein Kinases/analysis , Molecular Sequence Data , Mutation , Phosphorylation , Protein Interaction Maps , Rats , Sodium-Potassium-Exchanging ATPase/analysis , Transcription Factors/analysis , Transcription Factors/genetics
6.
Mol Cell ; 56(2): 232-245, 2014 Oct 23.
Article in English | MEDLINE | ID: mdl-25306918

ABSTRACT

Protein modification with ubiquitin chains is an essential signaling event catalyzed by E3 ubiquitin ligases. Most human E3s contain a signature RING domain that recruits a ubiquitin-charged E2 and a separate domain for substrate recognition. How RING-E3s can build polymeric ubiquitin chains while binding substrates and E2s at defined interfaces remains poorly understood. Here, we show that the RING-E3 APC/C catalyzes chain elongation by strongly increasing the affinity of its E2 for the distal acceptor ubiquitin in a growing conjugate. This function of the APC/C requires its coactivator as well as conserved residues of the E2 and ubiquitin. APC/C's ability to track the tip of an emerging conjugate is required for APC/C-substrate degradation and accurate cell division. Our results suggest that RING-E3s tether the distal ubiquitin of a growing chain in proximity to the active site of their E2s, allowing them to assemble polymeric conjugates without altering their binding to substrate or E2.


Subject(s)
Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome/metabolism , Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome/metabolism , Cdc20 Proteins/metabolism , Peptide Biosynthesis, Nucleic Acid-Independent/genetics , Ubiquitin-Conjugating Enzymes/metabolism , Ubiquitin/genetics , Catalytic Domain , Cdc20 Proteins/genetics , Cell Cycle Checkpoints/genetics , Cell Line, Tumor , Enzyme Activation , HeLa Cells , Humans , Protein Binding , Protein Structure, Tertiary , RNA Interference , RNA, Small Interfering , Ubiquitin/biosynthesis , Ubiquitination
7.
PLoS One ; 7(10): e46696, 2012.
Article in English | MEDLINE | ID: mdl-23056407

ABSTRACT

Elevated CO(2) levels (hypercapnia) occur in patients with respiratory diseases and impair alveolar epithelial integrity, in part, by inhibiting Na,K-ATPase function. Here, we examined the role of c-Jun N-terminal kinase (JNK) in CO(2) signaling in mammalian alveolar epithelial cells as well as in diptera, nematodes and rodent lungs. In alveolar epithelial cells, elevated CO(2) levels rapidly induced activation of JNK leading to downregulation of Na,K-ATPase and alveolar epithelial dysfunction. Hypercapnia-induced activation of JNK required AMP-activated protein kinase (AMPK) and protein kinase C-ζ leading to subsequent phosphorylation of JNK at Ser-129. Importantly, elevated CO(2) levels also caused a rapid and prominent activation of JNK in Drosophila S2 cells and in C. elegans. Paralleling the results with mammalian epithelial cells, RNAi against Drosophila JNK fully prevented CO(2)-induced downregulation of Na,K-ATPase in Drosophila S2 cells. The importance and specificity of JNK CO(2) signaling was additionally demonstrated by the ability of mutations in the C. elegans JNK homologs, jnk-1 and kgb-2 to partially rescue the hypercapnia-induced fertility defects but not the pharyngeal pumping defects. Together, these data provide evidence that deleterious effects of hypercapnia are mediated by JNK which plays an evolutionary conserved, specific role in CO(2) signaling in mammals, diptera and nematodes.


Subject(s)
Carbon Dioxide/toxicity , Epithelial Cells/drug effects , Epithelial Cells/enzymology , JNK Mitogen-Activated Protein Kinases/metabolism , Pulmonary Alveoli/cytology , Animals , Burkitt Lymphoma , Caenorhabditis elegans , Drosophila , Enzyme Activation/drug effects , Epithelial Cells/metabolism , Evolution, Molecular , Humans , JNK Mitogen-Activated Protein Kinases/genetics , Phosphorylation/drug effects , Protein Kinase C/metabolism , Rats , Sodium-Potassium-Exchanging ATPase/genetics , Sodium-Potassium-Exchanging ATPase/metabolism
8.
Trends Cell Biol ; 21(11): 656-63, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21978762

ABSTRACT

Modification of proteins with ubiquitin chains is an essential regulatory event in cell cycle control. Differences in the connectivity of ubiquitin chains are believed to result in distinct functional consequences for the modified proteins. Among eight possible homogenous chain types, canonical Lys48-linked ubiquitin chains have long been recognized to drive the proteasomal degradation of cell cycle regulators, and Lys48 is the only essential lysine residue of ubiquitin in yeast. It thus came as a surprise that in higher eukaryotes atypical K11-linked ubiquitin chains regulate the substrates of the anaphase-promoting complex and control progression through mitosis. We discuss recent findings that shed light on the assembly and function of K11-linked chains during cell division.


Subject(s)
Cell Division , Polyubiquitin/physiology , Anaphase-Promoting Complex-Cyclosome , Animals , Humans , Protein Multimerization , Ubiquitin-Protein Ligase Complexes/metabolism , Ubiquitinated Proteins/metabolism , Ubiquitination
9.
J Cell Sci ; 123(Pt 8): 1343-51, 2010 Apr 15.
Article in English | MEDLINE | ID: mdl-20332111

ABSTRACT

Stimulation of Na(+)/K(+)-ATPase translocation to the cell surface increases active Na(+) transport, which is the driving force of alveolar fluid reabsorption, a process necessary to keep the lungs free of edema and to allow normal gas exchange. Here, we provide evidence that insulin increases alveolar fluid reabsorption and Na(+)/K(+)-ATPase activity by increasing its translocation to the plasma membrane in alveolar epithelial cells. Insulin-induced Akt activation is necessary and sufficient to promote Na(+)/K(+)-ATPase translocation to the plasma membrane. Phosphorylation of AS160 by Akt is also required in this process, whereas inactivation of the Rab GTPase-activating protein domain of AS160 promotes partial Na(+)/K(+)-ATPase translocation in the absence of insulin. We found that Rab10 functions as a downstream target of AS160 in insulin-induced Na(+)/K(+)-ATPase translocation. Collectively, these results suggest that Akt plays a major role in Na(+)/K(+)-ATPase intracellular translocation and thus in alveolar fluid reabsorption.


Subject(s)
Alveolar Epithelial Cells/drug effects , Alveolar Epithelial Cells/enzymology , Cell Membrane/drug effects , Cell Membrane/enzymology , Insulin/pharmacology , Proto-Oncogene Proteins c-akt/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Animals , Body Fluids/drug effects , Body Fluids/enzymology , Cattle , GTPase-Activating Proteins/metabolism , Humans , Male , Phosphatidylinositol 3-Kinases/metabolism , Protein Transport/drug effects , Rats , Rats, Sprague-Dawley , Subcellular Fractions/drug effects , Subcellular Fractions/enzymology , rab GTP-Binding Proteins/metabolism
10.
Am J Physiol Lung Cell Mol Physiol ; 297(6): L1120-30, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19801454

ABSTRACT

Patients with acute lung injury develop hypoxia, which may lead to lung dysfunction and aberrant tissue repair. Recent studies have suggested that epithelial-mesenchymal transition (EMT) contributes to pulmonary fibrosis. We sought to determine whether hypoxia induces EMT in alveolar epithelial cells (AEC). We found that hypoxia induced the expression of alpha-smooth muscle actin (alpha-SMA) and vimentin and decreased the expression of E-cadherin in transformed and primary human, rat, and mouse AEC, suggesting that hypoxia induces EMT in AEC. Both severe hypoxia and moderate hypoxia induced EMT. The reactive oxygen species (ROS) scavenger Euk-134 prevented hypoxia-induced EMT. Moreover, hypoxia-induced expression of alpha-SMA and vimentin was prevented in mitochondria-deficient rho(0) cells, which are incapable of ROS production during hypoxia. CoCl(2) and dimethyloxaloylglycine, two compounds that stabilize hypoxia-inducible factor (HIF)-alpha under normoxia, failed to induce alpha-SMA expression in AEC. Furthermore, overexpression of constitutively active HIF-1alpha did not induce alpha-SMA. However, loss of HIF-1alpha or HIF-2alpha abolished induction of alpha-SMA mRNA during hypoxia. Hypoxia increased the levels of transforming growth factor (TGF)-beta1, and preincubation of AEC with SB431542, an inhibitor of the TGF-beta1 type I receptor kinase, prevented the hypoxia-induced EMT, suggesting that the process was TGF-beta1 dependent. Furthermore, both ROS and HIF-alpha were necessary for hypoxia-induced TGF-beta1 upregulation. Accordingly, we have provided evidence that hypoxia induces EMT of AEC through mitochondrial ROS, HIF, and endogenous TGF-beta1 signaling.


Subject(s)
Epithelial Cells/pathology , Hypoxia-Inducible Factor 1/metabolism , Hypoxia/pathology , Mesoderm/pathology , Mitochondria/metabolism , Pulmonary Alveoli/pathology , Reactive Oxygen Species/metabolism , Animals , Cell Line, Transformed , Epithelial Cells/metabolism , Gene Expression Regulation , Humans , Hypoxia/metabolism , Mesoderm/metabolism , Mice , Pulmonary Alveoli/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Transforming Growth Factor beta1/biosynthesis , Transforming Growth Factor beta1/genetics
11.
Mol Cell Biol ; 29(13): 3455-64, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19380482

ABSTRACT

Hypoxia promotes Na,K-ATPase endocytosis via protein kinase C zeta (PKC zeta)-mediated phosphorylation of the Na,K-ATPase alpha subunit. Here, we report that hypoxia leads to the phosphorylation of 5'-AMP-activated protein kinase (AMPK) at Thr172 in rat alveolar epithelial cells. The overexpression of a dominant-negative AMPK alpha subunit (AMPK-DN) construct prevented the hypoxia-induced endocytosis of Na,K-ATPase. The overexpression of the reactive oxygen species (ROS) scavenger catalase prevented hypoxia-induced AMPK activation. Moreover, hypoxia failed to activate AMPK in mitochondrion-deficient rho(0)-A549 cells, suggesting that mitochondrial ROS play an essential role in hypoxia-induced AMPK activation. Hypoxia-induced PKC zeta translocation to the plasma membrane and phosphorylation at Thr410 were prevented by the pharmacological inhibition of AMPK or by the overexpression of the AMPK-DN construct. We found that AMPK alpha phosphorylates PKC zeta on residue Thr410 within the PKC zeta activation loop. Importantly, the activation of AMPK alpha was necessary for hypoxia-induced AMPK-PKC zeta binding in alveolar epithelial cells. The overexpression of T410A mutant PKC zeta prevented hypoxia-induced Na,K-ATPase endocytosis, confirming that PKC zeta Thr410 phosphorylation is essential for this process. PKC zeta activation by AMPK is isoform specific, as small interfering RNA targeting the alpha1 but not the alpha2 catalytic subunit prevented PKC zeta activation. Accordingly, we provide the first evidence that hypoxia-generated mitochondrial ROS lead to the activation of the AMPK alpha1 isoform, which binds and directly phosphorylates PKC zeta at Thr410, thereby promoting Na,K-ATPase endocytosis.


Subject(s)
AMP-Activated Protein Kinases/metabolism , Endocytosis/physiology , Epithelial Cells/metabolism , Hypoxia/metabolism , Protein Kinase C/metabolism , Pulmonary Alveoli/cytology , Sodium-Potassium-Exchanging ATPase/metabolism , AMP-Activated Protein Kinases/genetics , Animals , Enzyme Activation , Epithelial Cells/cytology , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , Mitochondria/metabolism , Phosphorylation , Protein Kinase C/genetics , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Rats , Rats, Sprague-Dawley , Reactive Oxygen Species/metabolism , Signal Transduction/physiology , Sodium-Potassium-Exchanging ATPase/genetics
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