Social diffusion of new foraging techniques in the Southern ground-hornbill (Bucorvus leadbeateri).

Social learning during foraging has been found in a wide range of animals, including numerous bird species. Still, the mechanisms underlying this cognitive capacity remain largely unstudied and the use of divergent methods limits our understanding of their taxonomic distribution. Using an ecologically relevant design, the open diffusion experiment, we tested whether 11 Southern ground-hornbills (Bucorvus leadbeateri) were able to show imitation on the two-action task. Three experimental groups were created. In the slide and pull group, subjects ('observers') watched a trained conspecific ('demonstrator') opening a box using a specific technique. Naïve individuals from the control group, however, did not receive a social demonstration. All birds of the slide and pull group succeeded in opening the box, whereas all subjects of the control group failed the task. We found consistent inter-individual differences among some observers, with only two birds (one in each group) using the same technique and part of the box contacted by the demonstrator. Our results suggest that at least fine-tune enhancement underlies behavioural diffusion in this novel model species, which provides new research opportunities with direct implications for conservation.

Assessing sex differences in behavioural flexibility in an endangered bird species: the Southern ground-hornbill (Bucorvus leadbeateri).

Since ecology influences the expression of cognitive traits, intra-specific variation in ecological demands can drive differences in cognition. This is often the case, for instance, when sexes face different ecological challenges. However, so far, most studies have focused on few cognitive domains (i.e., spatial cognition), which limits our understanding of the evolution of sexually dimorphic cognition in animals. Endangered Southern ground-hornbills (Bucorvus leadbeateri), for example, show sex-specific ecological differences in age at dispersal, where females disperse from their natal group earlier than males. Based on this potential sex-specific source of selection, females and males may differ in their capacity to behave flexibly. Here, we used the reversal-learning paradigm in ten Southern ground-hornbills in two conditions: spatial and colour. During the pre-test (learning phase), regardless the sex, all subjects were faster at associating the food reward with spatial rather than with colour cues. Similarly, during the test (reversal-learning phase), both sexes learned the new association quicker with spatial cues. There were no sex differences in learning or reversal learning during both experimental phases. This possibility, however, requires further observation and experimentation. We hope our study will provide the impetus to assess further the cognitive capacities of this still overlooked species.

Assessing the spontaneous use of human-given cues in ground-hornbills.

A wide range of species relies on heterospecific visual cues to detect the location of resources like food. Although different hypotheses have been suggested to explain the emergence of this capacity in animals, results are often difficult to interpret due to the influence of other factors, such as close contact with humans. In this study, we presented eight Southern ground-hornbills (Bucorvus leadbeateri) with a two-way object-choice paradigm. The human experimenter provided a static and salient cue indicating which of the two containers was baited in four experimental conditions: (a) Touch, (b) Head, (c) Point + Head, and (d) Point. In an additional control condition (no cues given), we assessed whether subjects relied on olfactory cues to solve the task. Overall, our subjects did not spontaneously use human-given cues and their performance fluctuated near chance during the control condition. Noteworthy, most birds exhibited a side bias towards one container. We do not exclude the possibility that this species may be capable of using other cue types, or learning to use cues, after long interaction with humans is provided.

Through the eyes of a hunter: assessing perception and exclusion performance in ground-hornbills.

Logical inference, once strictly associated with spoken language, is now reported in some non-human animals. One aspect of logical inference, reasoning by exclusion, has been traditionally explored through the use of the cups task (cup A and cup B, if not cup A, then exclude cup A and select cup B). However, to fully understand the factors that drove the evolution of logical processes in animals, this latter paradigm needs to cover a taxonomically broader spectrum of species. In this study, we aimed to test the capacity of Southern ground-hornbills (Bucorvus leadbeateri) to show exclusion performance in a two-way object-choice task. First, we determined whether subjects could perceive and choose correctly between two containers (one rewarded, one unrewarded) using visual or acoustic cues (sensory phase). If successful, individuals were then presented with three experimental conditions (test phase): Full information (content of both cups revealed), Exclusion (content of the empty cup revealed), and Control (no content revealed). During the sensory phase, ground-hornbills succeeded in choosing the rewarded container only in the visual modality. Birds were able to select the rewarded container more than would be expected by chance in the Full information condition, but their performances were equal to chance in the Control condition. The without-learning performance of two individuals within the Exclusion condition indicates that this task is not trivial, which invites further investigation on this species' capacity to represent the dependent relationship between the cups (true logical inference).

Endocrine correlates of gender and throat coloration in the southern ground-hornbill (Bucorvus leadbeateri).

The southern ground-hornbill (SGH) is a cooperatively breeding bird endemic to eastern and southern Africa, but is endangered in its southern distributional range. The national conservation restoration program harvests redundant chicks for captive breeding and reintroduction; with sexing and social grouping of the species evaluated by throat-skin coloration, with adult males displaying a completely red color compared to dark blue within the red observed in adult females. However, recent findings indicate that dominant and subordinate adult males exhibit patches of blue throat-skin. To optimize SGH management practices, it is vital to determine the role of red and blue coloration, as well as the possible drivers thereof. As a prerequisite, an enzyme immunoassay for monitoring fecal androgen metabolite (fAM) concentrations in SGH was established. Following this, fresh fecal samples were collected from 78 SGH, of various demographics and origin, across 12 captive institutions, to determine whether fAM concentrations differ between blue (B), partially blue (sB), and fully red (R) throat-skin colored SGH. Furthermore, fAM concentrations were compared between males housed in different social groups of different age and sex classes. Individual median fAM concentrations of B, sB, and R adult males did not differ significantly but were considerably higher in B and sB males compared to R males. Social dynamics within captivity, for example, dominance, played no role as a driver of male gonadal activity or throat skin coloration. The results of the study indicate that androgens and apparent social dynamics are not primary determinants of throat coloration in male SGH.

Immunogenicity of Newcastle Disease Vaccine in Southern Ground-hornbill (Bucorvus leadbeateri).

The southern ground-hornbill (Bucorvus leadbeateri; hereafter SGH) is endangered in South Africa, Namibia, and Swaziland. Through a conservation program established in South Africa by the Mabula Ground Hornbill Project, wild populations are being re-established by the reintroduction of captive-reared birds. The SGH is susceptible to infection with avian avulavirus 1, which causes Newcastle disease (ND). Four different vaccines to protect against ND were administered through various vaccination schedules and evaluated by serologic monitoring to assess the efficiency and safety of various combinations of vaccines (live versus inactivated/killed), vaccine strains (Ulster strain, live; Avivac Cellimune, live; VG/GA strain, live; and Avivac Struvac, killed), and administration routes (intraocular versus subcutaneous versus intramuscular injection versus oral). We vaccinated 75 individuals and evaluated antibody titers in 53 individuals (24 juveniles, 13 subadults, and 16 adult SGH; 26 males and 27 females) over a period of 9 years. Antibody titers to avian avulavirus 1 in sera were monitored by a hemagglutination inhibition test. Protective titers were generated with 3/6 vaccine regimes tested in the SGH. The highest vaccine titers were established in birds vaccinated with the Ulster strain in the conjunctiva and followed with an intramuscular Struvac injection (mean log2 titer 8.6 ± 2.6) booster. Our aim was 1) to assess whether optimal vaccination protocols could be developed and 2) to then be able, by oral administration, to remove the need to recapture free-roaming, reintroduced birds to administer the initial vaccine or booster, thus remove the threat or mortality associated with ND to this endangered avian species in both captive birds and birds released back into the wild.

Lead Toxicosis in a Southern Ground Hornbill Bucorvus leadbeateri in South Africa.

The southern ground hornbill (Bucorvus leadbeateri) has been classified as globally vulnerable and, in South Africa, regionally endangered, with a negative population trend. Factors contributing to the population decline in South Africa are poisoning, electrocution, and illegal capture for trade, coupled with slow reproductive rates and extensive habitat requirements. Lead toxicosis is a previously undescribed threat for the population. An adult southern ground hornbill presented with acute lead toxicosis due to lead particles in the gizzard, which required intensive treatment. Two other hornbills were likely exposed. The source of the lead in these cases was likely a carcass of a porcupine that was killed with lead shot. This report highlights the importance of the use of lead-free ammunition within the habitat of the southern ground hornbill in South Africa.