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1.
Biotechnol Prog ; 26(2): 512-6, 2010.
Article in English | MEDLINE | ID: mdl-19938058

ABSTRACT

During this work, it was investigated whether spectral measurements can be used to monitor embryonic growth. An experiment was conducted in which both the transmission spectra and embryonic weight were determined on 240 eggs (Cobb, 37 weeks) between Day 5 and Day 10 of incubation. The spectral data were linked to embryonic weight by means of a partial least squares analysis. Different preprocessing procedures were compared during this work, that is, smoothing, multiplicative scatter correction (MSC), and first- and second-order derivative. Compared to the remainder of the preprocessing procedures, MSC leads to a considerable improvement of the prediction capability of the embryonic weight. The ratio of performance to deviation obtained for the MSC spectra equaled 4.5 indicating that a very accurate prediction of embryonic weight is feasible based on the VIS/NIR transmission measurements. Important regions for the prediction are situated around 685-740 nm. It is suggested that the spectral changes in these spectral regions result from the displacement of carotenoids from the yolk into the blood circuitry.


Subject(s)
Chick Embryo/growth & development , Light , Spectrum Analysis/methods , Animals , Chick Embryo/chemistry , Eggs , Least-Squares Analysis , Multivariate Analysis , Spectrum Analysis/instrumentation
2.
Biotechnol Prog ; 19(3): 1022-5, 2003.
Article in English | MEDLINE | ID: mdl-12790672

ABSTRACT

Coucke (1998) was the first to use acoustic resonance analysis to monitor embryo development in chicken eggs. He remarked that at around 100 hours of incubation, the course of the resonant frequency and damping changed abruptly in the case of fertile eggs. He also showed that these changes were related to a physiologic event during early embryonic development. The objective of our study is to monitor the course of the vibration parameters during the early incubation of chicken eggs and to relate these changes to egg and embryo characteristics. A total of 72 Hybro eggs were incubated vertically in a small incubator at standard conditions. Several egg parameters were measured before incubation. During the early stages of incubation the vibration behavior of these eggs was monitored. The time at which the damping of the vibration suddenly changed, the diameter of the eggs and their interaction were found to be significant explanatory variables in order to predict hatching time. A correlation coefficient r of 0.72 was obtained.


Subject(s)
Chick Embryo/growth & development , Embryonic and Fetal Development/physiology , Movement/physiology , Oscillometry/methods , Physical Stimulation/instrumentation , Physical Stimulation/methods , Vibration , Zygote/physiology , Animals , Chickens , Incubators , Pilot Projects , Statistics as Topic
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