[Comparative studies of acid-base and gas metabolism in the whole blood and erythrocytes in endotoxic shock in rabbits]. / Sravnitelni prouchvaniia vurkhu kiselinno-alkalniia i gazoviia metabolizum na tsialostnata kruv i eritrotsiti pri endotoksinov shok u zaitsi.

Parameters of acid-base and gas metabolism in whole blood and in red cell hemolysate were determined 30, 60 and 120 minutes after inducing endotoxin shock in rabbits of Belgian giant breed. Astrup's micromethod on apparatus of the firm "Radiometer" was used. Endotoxin shock was induced by intravenous injection of 2 mg/kg endotoxin from E. coli 0111:B4 strain. Equations were used for expressing the relation between pH of erythrocytes and pH of whole blood and analysis made of the correlation between the changes in the individual components of erythrocyte and of whole blood acid-base metabolism. Severe metabolic acidosis developed both in whole arterial blood, in mixed venous blood and in red cell hemolysate. Close correlation was recorded between the changes in the parameters of acid-base metabolism in whole blood and in red cell hemolysate on the 60. and especially on the 120. minute after endotoxin administration. The blood gas changes manifested by slight decrease of PCO2 and PO2 both in arterial and in mixed venous whole blood and red cell hemolysate were not statistically significant. The severe tissue hypoxia during the early phases of endotoxin shock was thought to be the result of severe hemodynamic changes.

Influence of immobilization stress on the phospholipid composition of alveolar surfactant and lungs in rats.

The influence of immobilization stress on the lipid composition of alveolar surfactant and lungs in rats immobilized for 12 and 24 hours, the effects of phospholipase A2, and lipid transfer activity in alveolar surfactant were investigated. The results indicate that alveolar surfactant phospholipids underwent more significant alterations compared to lung phospholipids. Furthermore, phospholipase A2 and lipid transfer activity were reduced in alveolar surfactant of immobilized rats. The reported data suggest that the lower lipid transfer activity might be responsible for the reduced phospholipids in the surfactant system.