ABSTRACT
The method of obtaining and rearing germfree miniature piglets for medical-biological research is described. The gnotobiotes were reared in rigid organic glass isolation cells up to 3 months. Milk diets supplemented with minerals and vitamins were used for nutrition. The gnotobiotes gained weight well during the whole observation period.
Subject(s)
Germ-Free Life , Swine/growth & development , Animal Husbandry/methods , AnimalsABSTRACT
Dilatation of the endoplasmic reticulum cavities, an increase in the number of ribosomes near bacteria, deformation of mitochondria and coarsening of cristae were revealed in phagocytosis of Past. pestis, strain EV, by reticular cells in the tissue culture of the spleen of intact guinea pigs. Lipophanerous "reticular" inclusions were found in the differentiated reticular cells of the infected cultures. In the reticular cell cytoplasma besides the intact bacteria there were revealed Past. pestis at the initial stages of involution.