ABSTRACT
Changes in the fractional composition of lipids isolated from millet, rice and semolina artificially infected with the microscopic fungus Fusarium sporotrichiella Bilai were followed. The lipid complex was found to undergo marked changes and to yield toxic sterololactones.
Subject(s)
Edible Grain/microbiology , Food Microbiology , Fusarium/pathogenicity , Lipids/analysis , Animals , Lactones/toxicity , Lipids/toxicity , Oryza/analysis , Panicum/analysis , Rabbits , Skin Tests , Sterols/toxicity , Triticum/analysisABSTRACT
The chromatographic method for determining the toxicity of the cereal crop grain affected by micromycates F. sporotrichiella is proposed. The method is based on the separation of the lipids extracted from the grain under examination in a thin layer of the silica gel and detection on the chromatogram of two toxic fractions with Rf-0.06 and 1.22 fluorecent in the UV-light by emitting greyish-green and emerald-green coloured light. The chromatographic method is more sensitive, visually obvious and quicker by comparison with the biological ones.