ABSTRACT
Neogen Corp. developed the Veratox aflatoxin test kit for the detection of total aflatoxin. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are several AOAC Official Methods for total aflatoxin detection in corn (994.08, 990.33, 979.18, 993.17, 990.32, 993.16, 991.31, and 990.74), varying between rapid and analytical-based methods and one rapid method that has been performance tested by the AOAC Research Institute (PTM 030701). However, the widely used reference method is AOAC Official Method 994.08, which is an HPLC method and is referred to as the reference method in this paper. Although considered the reference method, the HPLC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit to be validated by the AOAC Research Institute is needed.
Subject(s)
Aflatoxins/analysis , Enzyme-Linked Immunosorbent Assay/methods , Food Analysis/methods , Food Contamination , Zea mays/metabolism , Agriculture , Chemistry Techniques, Analytical , Chromatography, High Pressure Liquid/methods , Cross Reactions , Mycotoxins/metabolism , Reproducibility of Results , Time FactorsABSTRACT
Neogen Corp. developed the Veratox DON test kit for the detection ofdeoxynivalenol (DON). The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are two AOAC Official Methods for DON detection: 986.17 and 986.18, the first of which is a TLC method and the second a GC method. A rapid method (PTM 000701) has also been performance tested by the AOAC Research Institute. One of the most widely used reference methods; however, is a GC method with electron capture detection that is referred to as the reference method in this paper. Although considered the reference method, the GC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit needs to be validated by the AOAC Research Institute. The Veratox 2/3 method is highly reproducible with average CV values < 10%, and is very accurate, showing > 97% correlation to reference methods.
Subject(s)
Avena/metabolism , Enzyme-Linked Immunosorbent Assay/methods , Food Analysis/methods , Food Contamination , Hordeum/metabolism , Oryza/metabolism , Trichothecenes/analysis , Triticum/metabolism , Zea mays/metabolism , Chemistry Techniques, Analytical , Chromatography, Gas/methods , Laboratories/standards , Reagent Kits, Diagnostic , Reproducibility of Results , Temperature , United States , United States Food and Drug AdministrationABSTRACT
A lateral flow immunoassay for Escherichia coli O157:H7 (Reveal E. coli O157:H7, 20-h version), previously validated for 25 g raw ground beef, raw beef cubes, apple cider, lettuce rinse, and environmental swab samples, has been validated for use in testing 375 g samples of raw ground beef and raw beef cubes. The Reveal method, including culture confirmation of positive immunoassay results, was evaluated in comparison to the current reference culture procedure of the U.S. Department of Agriculture-Food Safety and Inspection Service. For raw ground beef, the Reveal and reference methods produced the same number of confirmed positive results. For raw beef cubes, the Reveal method produced more positives than the reference method, but this difference was not statistically significant. The Reveal method exhibited 100% specificity, with no false-positive results obtained on uninoculated control samples. It is recommended that this minor modification to Method 2000.14 be adopted Revised First Action.
Subject(s)
Bacteriological Techniques/methods , Escherichia coli O157/isolation & purification , Food Microbiology , Immunoassay/methods , Meat/microbiology , Animals , Bacteriological Techniques/standards , Bacteriological Techniques/statistics & numerical data , Cattle , Colony Count, Microbial , Environmental Microbiology/standards , Food Microbiology/standards , Immunoassay/standards , Immunoassay/statistics & numerical data , Sensitivity and Specificity , Time Factors , United States , United States Department of AgricultureABSTRACT
A lateral flow immunoassay for Escherichia coli O157:H7 (Reveal E. coli O157:H7, 8-h version), previously validated for 25 g raw ground beef, raw beef cubes, and lettuce rinse samples, has been validated for use in testing 375 g samples of raw ground beef and raw beef cubes. The Reveal method, including culture confirmation of positive immunoassay results, was evaluated in comparison to the current reference culture procedure of the U.S. Department of Agriculture-Food Safety and Inspection Service. For both sample types, the Reveal test with 12-h enrichment produced more positives than the reference method, although the differences were not statistically significant. Statistical equivalence to the reference method was also obtained at 10 and 11 h of enrichment for raw beef cubes and at 8, 10, and 11 h for raw ground beef, but maximum sensitivity of the method is only achieved after 12 h of enrichment. The Reveal method exhibited 100% specificity, with no false-positive results obtained on uninoculated control samples. It is recommended that this minor modification to Method 2000.13 be adopted Revised First Action.
