ABSTRACT
We retrospectively studied 13 patients with degenerative change associated with a scaphoid nonunion treated by internal fixation and bone graft. All patients had radiological signs of radioscaphoid degenerative change (scaphoid nonunion advanced collapse--scaphoid nonunion advanced collapse-- grade II or more) and had surgery more than 2 years after injury. Ten of the 13 patients achieved union at 6 months with all but one of these demonstrating improvement on the Disability of Arm, Shoulder and Hand (DASH) score. Surgeons presented with this difficult patient group might consider reconstruction before salvage procedures.
Subject(s)
Bone Transplantation/methods , Fracture Fixation, Internal/methods , Fractures, Bone/surgery , Fractures, Ununited/surgery , Ilium/transplantation , Scaphoid Bone/injuries , Disability Evaluation , Female , Fractures, Ununited/diagnostic imaging , Humans , Male , Radiography , Retrospective Studies , Salvage Therapy , Scaphoid Bone/diagnostic imaging , Treatment OutcomeSubject(s)
Bone Plates , Fracture Fixation, Internal/instrumentation , Humeral Fractures/surgery , Joint Prosthesis , Aged , Arthroplasty, Replacement , Elbow Joint/surgery , Female , Fracture Fixation, Internal/methods , Fracture Healing , Humans , Humeral Fractures/diagnostic imaging , Radiography , Shoulder Joint/surgeryABSTRACT
The uptake of methylamine and of methanol by the facultative methylotroph Pseudomonas sp. strain AM1 was investigated. It was found that this organism possesses two uptake systems for methylamine. One of these operates when methylamine is the sole source of carbon, nitrogen, and energy. It has a Km of 1.33 X 10(-4) M and a Vmax of 67 nmol/min per mg of cells (dry weight). The other system, found when methylamine is the sole nitrogen source only, has a Km of 1.2 X 10(-5) M and a Vmax of 8.9 nmol/min per mg of cells (dry weight). Both uptake systems were severely inhibited by azide, cyanide, carbonyl cyanide-m-chlorophenyl hydrazone, and N-ethylmaleimide, but only the high-affinity system was inhibited by ammonium ions with a Ki of 7.7 mM. Both systems were susceptible to osmotic shock treatment, competitively inhibited by ethylamine, and unaffected by most amino acids. Methanol uptake showed a Km of 4.8 microM and a Vmax of 60.6 nmol/min per mg of cells (dry weight) and was not inhibited by osmotic shock treatment. Azide, cyanide, and N-ethylmaleimide curtailed uptake, but carbonyl cyanide-m-chlorophenyl hydrazone merely reduced the rate of uptake. A methanol dehydrogenase mutant, M15A, was unable to take up methanol. It is proposed that methanol diffuses into the cell where it is rapidly oxidized by methanol dehydrogenase.
