ABSTRACT
Treatment results in 107 patients with incisional hernias were analyzed. All patients undergoing abdominal wall grafting with use of cellular polypropylene implant. Patients were divided into 2 groups. 57 patients operated according alloplastic method sublay formed the first group, 50 patients operated by method onlay formed the second group. Frequency of local wound postoperative complications and hernia relapses were taken into account for comparative evaluation of surgical treatment results. Hernia relapses investigated in 51 patients from the first group and in 44 patients from the second group. Basing on the authors' data method sublay are accompanied by local wound complications (infiltration, suppuration, seroma, haematoma) in 12,3% of observations, method onlay - in 28% (x(2)=4,17, p=0,04). In the first group hernia relapse developed in 1 patient (2%), in the second group - in 3 patients (6,8%) (x(2)=1,43, p=0,23). Authors consider that treating median incisional hernias by using diverse alloplastic methods preference should be given to sublay method.
Subject(s)
Hernia, Ventral/etiology , Hernia, Ventral/surgery , Polypropylenes , Postoperative Complications , Surgical Mesh , Female , Hernia, Ventral/diagnosis , Humans , Male , Middle Aged , Severity of Illness IndexABSTRACT
YPEL1 is a nuclear protein that is suggested to be involved in mesenchymal to epithelial-like transition during tissue development. Recently we have identified YPEL1 as a gene whose expression is deregulation in perineural invasive pancreatic cancer cells. In this study we assessed the expression of YPEL1 in normal and diseased pancreatic tissues and pancreatic cancer cell lines. Quantitative real time polymerase chain reaction was used to analyze the expression of YPEL1 mRNA in nine cultured pancreatic cancer cell lines and pancreatic bulk tissues of the normal pancreas (n=19), chronic pancreatitis (n=19) and pancreatic adenocarcinoma tissues (n=31). Quantitative real time polymerase chain reaction analysis revealed a significant down-regulation of YPEL1 mRNA expression in pancreatic adenocarcinoma tissues compared to normal tissues (54.1+/-5.2 vs. 85.8+/-14.1 copies/10,000 copies cpb) and low expression of this gene indicated a tendency for better survival of pancreatic cancer patients (16 vs. 13 months; p=0.17). Expression of YPEL1 mRNA was present in all tested pancreatic cancer cell lines with comparably low to moderate expression levels of 4.3 - 88.0 copies/10,000 copies cpb. Reduced expression of YPEL1 in pancreatic cancer might be related to perineural invasion. and prognosis. YPEL1 might be an important factor during the development and malignant transformation of tissues. Further studies are required to better assess the role of human YPEL1 in pancreatic cancer pathogenesis.
Subject(s)
Gene Expression/genetics , Pancreatic Neoplasms/genetics , Pancreatic Neoplasms/pathology , Cell Line, Tumor , Fibroblasts/metabolism , Humans , Pancreatic Neoplasms/metabolism , Phenotype , Tumor Cells, CulturedABSTRACT
BACKGROUND: Pancreatic infiltration by leucocytes represents a hallmark in acute pancreatitis. Although leucocytes play an active role in the pathophysiology of this disease, the relation between leucocyte activation, microvascular injury and haemorrhage has not been adequately addressed. METHODS: We investigated intrapancreatic leucocyte migration, leucocyte extravasation and pancreatic microperfusion in different models of oedematous and necrotising acute pancreatitis in lys-EGFP-ki mice using fluorescent imaging and time-lapse intravital microscopy. RESULTS: In contrast to the current paradigm of leucocyte recruitment, the initial event of leucocyte activation in acute pancreatitis was represented through a dose- and time-dependent occlusion of pancreatic capillaries by intraluminally migrating leucocytes. Intracapillary leucocyte accumulation (ILA) resulted in dense filling of almost all capillaries close to the area of inflammation and preceded transvenular leucocyte extravasation. ILA was also initiated by isolated exposure of the pancreas to interleukin 8 or fMLP, demonstrating the causal role of chemotactic stimuli in the induction of ILA. The onset of intracapillary leucocyte accumulation was strongly inhibited in LFA-1(-/-) and ICAM-1(-/-) mice, but not in Mac-1(-/-) mice. Moreover, prevention of intracapillary leucocyte accumulation led to the development of massive capillary haemorrhages and transformed mild pancreatitis into lethal haemorrhagic disease. CONCLUSIONS: ILA represents a novel protective and potentially lifesaving mechanism of haemostasis in acute pancreatitis. This process depends on expression of LFA-1 and ICAM-1 and precedes the classical steps of the leucocyte recruitment cascade.
Subject(s)
Capillaries , Chemotaxis, Leukocyte/physiology , Hemorrhage/blood , Hemostasis/physiology , Leukocytes/physiology , Pancreas/blood supply , Pancreatitis/blood , Acute Disease , Animals , Chemotactic Factors/administration & dosage , Intercellular Adhesion Molecule-1/metabolism , Lymphocyte Function-Associated Antigen-1/metabolism , Male , Mice , Microcirculation/immunology , Pancreas/chemistry , Pancreatitis/pathologyABSTRACT
In the present study authors have analyzed the suitability of the infrared (IR) spectroscopy for diagnosis of morphological and functional changes of the stomach in ulcer pyloroduodenal stenosis. Data obtained from 64 patients have shown that the IR spectroscopic features of the stomach were dependent on the stage of stenosis, secretory function of the stomach and the presence of the Helicobacter Pylori (HP) infection. IR spectroscopy can be a multi-purpose mean of assessment of the morphological and functional properties of the stomach in pyloroduodenal stenosis. IR spectroscopy provides the opportunity to assess the secretory state of the stomach at different stages of the disease. Using IR spectroscopy we were able to reveal the presence of HP in the stomach and the dynamics of its eradication during of conservative treatment and at any time after surgical intervention.
