Membrane lipid composition of Carnobacterium maltaromaticum CNCM I-3298, a highly cryoresistant lactic bacterium.

The growing consumption of fermented products has led to an increasing demand for lactic acid bacteria (LAB), especially for LAB tolerant to freezing/thawing conditions. Carnobacterium maltaromaticum is a psychrotrophic and freeze-thawing resistant lactic acid bacterium. The membrane is the primary site of damage during the cryo-preservation process and requires modulation to improve cryoresistance. However, knowledge about the membrane structure of this LAB genus is limited. We presented here the first study of the membrane lipid composition of C. maltaromaticum CNCM I-3298 including the polar heads and the fatty acid compositions of each lipid family (neutral lipids, glycolipids, phospholipids). The strain CNCM I-3298 is principally composed of glycolipids (32%) and phospholipids (55%). About 95% of glycolipids are dihexaosyldiglycerides while less than 5% are monohexaosyldiglycerides. The disaccharide chain of dihexaosyldiglycerides is composed of α-Gal(1-2)-α-Glc chain, evidenced for the first time in a LAB strain other than Lactobacillus strains. Phosphatidylglycerol is the main phospholipid (94%). All polar lipids are exceptionally rich in C18:1 (from 70% to 80%). Regarding the fatty acid composition, C. maltaromaticum CNCM I-3298 is an atypical bacterium within the genus Carnobacterium due to its high C18:1 proportion but resemble the other Carnobacterium strains as they mostly do not contain cyclic fatty acids.

[Radiofrequency ablation of atrioventricular conduction during the 5th month of pregnancy]. / Interruption de la conduction auriculo-ventriculaire par radiofréquence au 5e mois d'une grossesse.

The authors report the case of radiofrequency ablation of atrioventricular conduction in a 5 months pregnant woman who had hypertrophic cardiomyopathy. The indication of this procedure was a poorly tolerated resistant supraventricular tachycardia with foetal distress. A dual-chamber rate-assisted pacemaker programmed in the VVIR mode was implanted during the same procedure normally, with normal delivery of a healthy child at 8 months' gestation.

[Long-term outcome of a hospital series of patients with atrio-ventricular accessory pathway]. / Devenir à long terme d'une série hospitalière de patients ayant une voie de conduction accessoire auriculo-ventriculaire.

Ninety five patients with a mean age of 39 +/- 19 years, 82 of whom were symptomatic, having an accessory atrioventricular bidirectional conduction pathway (WPW syndrome: 77; "concealed": 18) were followed up for an average of 7.3 +/- 2.6 years. The objectives were to analyse: the incidence and causes of death and the possible predictive factors of death due to the WPW syndrome--the influence of medical treatment and type of medication on survival and symptoms. Of the 8 cardiac deaths, 6 seemed to be related to the WPW syndrome, a prevalence of 7.8% and an annual incidence of 1.1/1000. The main risk factors which were identified were: age 62 +/- 8 years versus 37 +/- 15 years in survivors; p < 0.02--associated organic heart disease, especially ischaemic heart disease (5/6)--the description of severe symptoms, in particular recurrent syncope--documented malignant spontaneous or induced arrhythmias (5/6)--anterograde AV conduction with an effective refractory period < or = 230 msec in 4, though it was only 270 msec in the other 2 patients, indicating that this parameter is not specific--amiodarone (6/6) did not prevent the fatal outcome in this particular group of patients. In the "benign" forms, only betablocker drugs could significantly reduce the frequency and severity of symptoms, especially when compared with Class I or IC antiarrhythmics. These results suggest that the indications of radical treatment should be widened in high risk patients, especially when elderly and with associated coronary artery disease. They also suggest that the role of betablocker drugs should be reevaluated in the so-called "benign" symptomatic forms.

[Influence of exercise on the permeability of accessory pathways and supraventricular arrhythmia in Wolff-Parkinson-White syndrome]. / Influence de l'effort sur la perméabilité des voies accessoires et le comportement des arythmies supraventriculaires dans le syndrome de Wolff-Parkinson-White.

The influence of adrenergic stimulation on the effective anterograde refractory period of the accessory pathways and on supraventricular arrhythmias, was studied in 20 patients (average age 38 +/- 16 years) with an untreated permanent Wolff-Parkinson-White syndrome and a resting anterograde refractory period < or = 400ms. Repeated electrophysiological studies with a single endocavity catheter positioned near the atrial pole of the accessory pathway were performed under basal conditions and during a standardised exercise test on a bicycle ergometer. The effective anterograde refractory period of the accessory pathway, the length of the tachycardia cycle during reciprocating orthodromic tachycardia, the average heart rate, the percentage of preexcited QRS complexes during induced atrial fibrillation, were measured in all patients under basal conditions and at the peak of exercise. Exercise significantly reduced the anterograde refractory period of the accessory pathway (287 +/- 49 ms at rest versus 238 +/- 24 ms on exercise: p < 0.001), the cycle of orthodromic tachycardia (302 +/- 32 vs 260 +/- 22 ms p < 0.001), the minimal R-R interval (270 +/- 65 vs 227 +/- 46 ms: p < 0.05) and % of preexcited QRS complexes (75 +/- 33 vs 51 +/- 39: p < 0.05) in atrial fibrillation whilst increasing the average heart rate (165 +/- 42 vs 202 +/- 39 bpm: p < 0.02). Adrenergic stimulation significantly improves anterograde conduction in the accessory pathway. The reduction in the % of preexcited QRS complexes in atrial fibrillation could indicate a preferential action of catecholamines on the nodo-hisian pathway.(ABSTRACT TRUNCATED AT 250 WORDS)

[Value of an algorithm of automatic adaptation of the atrio-ventricular delay to the instantaneous atrial rate in cardiac stimulation]. / Intérêt d'un algorithme d'adaptation automatique du délai auriculo-ventriculaire à la fréquence atriale instantanée en stimulation cardiaque DDD.

It has been suggested that an algorithm of automatic adaptation of the AV delay to the instantaneous atrial rate be introduced into the program of DDD pacemakers to reproduce the physiological adaptation of the PR interval to effort, characterised by progressive shortening inversely linearly related to the heart rate. In order to evaluate the potential benefits in conditions of "standard" programming (basal AV delay the same for all patients: maximal frequency of 1/1 AV synchronisation uniformly limited to 120 bpm), a haemodynamic study was undertaken in 10 patients who had permanent DDD pacemakers implanted for advanced AV block. Measurements were taken during two standardized exercise stress tests (20 W/2 mn steps from an initial load of 20 W) performed in a random order, one with a fixed AV delay of 156 ms and the other with an "automatic AV delay" allowing linear reduction from a maximum value of 156 ms at rest to a minimum value of 84 ms at the maximum heart rate of 120 bpm. At the peak of exercise the "automatic AV delay" significantly affected 4 parameters: the paced ventricular rate (p = 0.008) and rate-pressure product (p = 0.005) which increased, pulmonary capillary pressure (p = 0.03) and cycle-to-cycle variability of systolic and diastolic blood pressures (p = 0.02 < p < 0.0001) which decreased. There was a tendency (NS) to slowing of the spontaneous atrial rate and to increase in cardiac output. This increase was significant in some patients and seemed to be due to a good relationship between the individual optimal value of the value basal AV delay measured by Doppler echocardiography and the value programmed in this study (156 ms).(ABSTRACT TRUNCATED AT 250 WORDS)

[Doppler echocardiography and double chamber pacing]. / Echo-Doppler cardiaque et stimulation double-chambre.

Doppler-echocardiography is playing an increasing role in cardiac pacing: 1) Before implantation, to determine any cardiac disease possibly accompanying the conduction disturbance, and the quality of atrial function in order to identify the appropriate indications for the type of pacing which will restore normal AV synchronism. Alongside morphometric data (size of atria, etc.), this analysis is based above all on the evaluation of LV filling flows and ejection, if necessary during provisional pacing in DRV mode. It is important to be aware of and prevent certain problems: 1st degree AV block with very long PR, high degree interatrial conduction disturbances, etc. 2) To evaluate the possible benefits of pacing in certain new indications, e.g. obstructive hypertrophic cardiomyopathy (measurement of intra-LV gradient in sinus rhythm and with DRV pacing with total ventricular capture). 3) After implantation, to optimise the programming of double-chamber pacemakers and in particular AV intervals (base-line AV interval with paced atrial cycle, AV interval with detected atrial cycle, hysteresis of AV interval corresponding to the difference between the two previous values, slope of automatic variation in AV interval during exercise, etc.). Individual programming of these parameters based upon analysis of transmitral and ejection flow rates, at rest and, if necessary, during exercise, enables the optimisation of cardiac function (which is above all useful in the presence of concomitant organic heart disease) while at the same time improving the electrophysiological behaviour of the pacemaker at high frequencies.

Lack of physiological adaptation of the atrioventricular interval to heart rate in patients chronically paced in the AAIR mode.

Seventeen consecutive patients, aged 56 +/- 12, were chronically paced in the AAIR mode for a symptomatic sinus node disease with atrial chronotropic incompetence defined by a peak exercise heart rate (HR) less than 75% of the maximal predicted heart rate (MPHR) mean = 65 +/- 10%). Sensors used were activity sensing (n = 7), minute ventilation (n = 6), or respiratory rate (n = 4). Basic pacing rate was programmed at 71 +/- 5 beats/min and the maximal sensor rate at approximately 85% MPHR (143 +/- 10); other sensor parameters were programmed individually. Six months after implant, two standardized and symptom limited exercise tests were performed in random order, AAI and AAIR modes, respectively. AAIR pacing significantly improved peak exercise HR (139 +/- 14 vs 112 +/- 30 beats/min; P less than 0.01), maximal sustained workload (132 +/- 42 vs 110 +/- 38 watts; P less than 0.02), and total exercise duration (724 +/- 299 vs 594 +/- 245 sec; p less than 0.02) compared to the AAI mode. In all 17 patients, HR was continuously sensor driven in the AAIR mode, making it possible to precisely study the adaptation of the stimulus-R interval and of the stimulus-R:RR ratio during exercise. Six patients normally adapted with a progressive shortening. Six others did not adapt at all without any variation of interval. Five patients paradoxically increased their stimulus-R interval (286 +/- 10 msec at peak E vs 220 +/- 19 msec at rest) and their stimulus-R:RR ratio (67 +/- 20% vs 29 +/- 4%), producing P waves occurring immediately after, or even within the R wave of the preceding cycle; two patients complained of severe exercise related symptoms corresponding to the so-called "AAIR pacemaker syndrome." The principal factors involved in the nonadaptation of AV interval to HR were related to the patient (organic heart disease, with the particular problem of the denervated heart; the bradytachy syndrome; and the use of drugs, especially beta blockers and Class I antiarrhythmic drugs) or to the pacemaker ("overstimulation" phenomenon). These observations constitute an additional argument for wider indications of implanting DDDR units in these patients.

Predators of the tick Amblyomma variegatum (Acari: Ixodidae) in Guadeloupe, French West Indies.

Some vertebrate species in Guadeloupe are predators of free or parasitic stages of Amblyomma variegatum (Fabricius). Among birds, 1.9% of the 421 identified animals found in the stomachs of grackles (Quiscalus lugubris), 1.6% of the 364 animals found in the stomachs of free-ranging chickens, and 0.3% of the 4642 animals found in the stomach of cattle egrets (Bubulcus ibis) were A. variegatum ticks. The most efficient predator of ticks was the tropical fire ant, Solenopsis geminata, which was observed to only attack engorged stages of ticks. An average of 8% of the 564 individual ticks or batch of ticks released on the ground, mainly in a grass environment, were attacked by this ant species. Mice (Mus musculus) and mongooses (Herpestes auropunctatus) feed on engorged nymphs and female ticks. None of the 15 strains of entomophagous nematodes, genera Steinernema and Heterorhabditis, experimentally put in contact with engorged larvae and nymphs were able to parasitize them. In the laboratory, the insects Megaselia scalaris (Diptera) and Tineola sp. (Lepidoptera) were occasionally found to feed on engorged and unfed ticks, respectively.

[New cardiac pacemakers]. / Les nouveaux stimulateurs cardiaques.

Because of the extraordinary technological progress made over the past years in pulse generators, the objective and indications of definitive cardiac pacing have greatly changed. All the different advantages are aimed at mimicking the electrophysiological and mechanical conditions of normal heart function. We have seen the development of: DDD mode dual chamber pacemakers, which in cases of AV block recreate normal AV synchrony; single chamber rate-responsive units piloted by an external captor responding to the organism's metabolic needs (intra-corporal vibrations resulting from exercise, respiratory rate, minute ventilation, QT interval, central temperature, SV O2...); they provide effective correction of atrial (AAI-R mode) or ventricular (VVI-R mode) chronotopic incompetencies often associated with chronic conduction disorders which make it impossible for patients paced in VVI or AAI mode to adapt heart rate to exercise level; and finally, "universal" pacemakers capable of pacing in all the single or dual chamber modes with rate responsiveness to one or several captors. These new pacemakers offer adaptability to the paced patient's needs as they vary throughout his lifetime. These units will probably be the "standard" cardiac pacemakers of the future.

Invasion of Tomato Roots and Reproduction of Meloidogyne incognita as Affected by Raw Sewage Sludge.

The antagonistic effects of raw sewage sludge on infection of tomato by Meloidogyne incognita were tested in greenhouse pot experiments. Sludge was mixed with the soil or added on its surface before and after inoculation of tomato plants with nematode eggs. Juvenile penetration was determined 1 and 10 days after inoculation, and 6 weeks later root systems were assessed for nematode reproduction. Fewer juveniles penetrated roots in pots with sludge added to the soil than in unamended control pots. In both experiments, roots were severely galled despite a significant reduction in gall ratings in amended relative to unamended soils. Egg production in treated soil was less (P = 0.05) than in control pots, regardless of whether sludge was incorporated or added 1 day before or after inoculation. In treated pots, RF values (final egg number/inoculation egg number) were strongly reduced. The toxic effects observed on the parasite may result from the ammoniacal nitrogen released in the soil within 7 days after treatment, associated with possible poor host suitability of tomatoes grown in amended substrate and short-lasting compound(s) active after root invasion.

[Spermiogenesis and the function of the vaginal sac in Ichthyocephalus anadenoboli. n. sp. (Rhigonematidae; Nematoda), a parasite of Anadenobolus politus (Porat) (Rhinocricidae; Diplopoda) in Guadeloupe]. / Spermiogénèse et fonction du sac vaginal chez Ichthyocephalus anadenoboli n. sp. (Rhigonematidae; Nematoda), parasite d'Anadenobolu s politus (Porat) (Rhinocricidae; Diplopoda) en Guadeloupe.

Ichthyocephalus anadenoboli n. sp. ( Rhigonematidae ; Nematoda) from Anadenobolus politus ( Porat ) ( Rhinocricidae ; Diplopoda ) from Guadeloupe is described. The new species most closely resembles I. egleri since both sexes are of approximately the same size and the spicules are markedly unequal; it is distinguished in having a much longer right spicule, by the form of the extremity of the left spicule and by the presence of a vaginal sac opening into the vagina at its junction with the uteri. In addition certain aspects of spermiogenesis in the new species are studied. Maturation of spermatids occurs in two phases, one in the testis and seminal receptacle of males, the second in the vaginal sac in females. The second phase involves profound changes in form as well as nuclear transformation. It is suggested that the vaginal sac observed in certain species of Rhigonema as well as the " bourse copulatrice " of certain Tetrameres spp. and Maupasina weissi , like that of Ichthyocephalus anadenoboli , are specialized to accommodate the final stages of spermiogenesis.