ABSTRACT
Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.
Subject(s)
Glutens/genetics , Glutens/immunology , Immunoglobulin E/immunology , Wheat Hypersensitivity/immunology , Amino Acid Sequence , Anaphylaxis/immunology , Chimera , Escherichia coli , Exercise , Glutens/chemistry , Humans , Molecular Sequence Data , Molecular Weight , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Proteins/immunology , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Wheat Hypersensitivity/diagnosisABSTRACT
We report two patients with hypomelanosis of Ito, one whose afflictions were limited to the pigmentary skin abnormalities and another with systemic involvement of the neurological and gastrointestinal system. These case reports give support to the importance of establishing hypomelanosis of Ito as occurring with or without systemic involvement.
Subject(s)
Hypopigmentation/pathology , Pigmentation Disorders/pathology , Adult , Child, Preschool , Female , Gastrointestinal Diseases/complications , Gastrointestinal Diseases/pathology , Humans , Hypopigmentation/complications , Nervous System Diseases/complications , Nervous System Diseases/pathology , Pigmentation Disorders/complicationsABSTRACT
Merkel-cell trabecular carcinoma of the skin has a prognosis poorer than expected for a small skin lesion. Surgery is central to actual treatment. A wide excision is indicated for localized disease. For selected cases we believe that an extensive resection of the draining lymphatics in continuity with the tumor should be performed. We have called this procedure "lymphangiectomy" in comparison to lymphadenectomy when the lymph nodes are removed. An early diagnosis and prompt treatment can contribute to improve the survival for this aggressive skin tumor.
