Nano zinc oxide mediated resuscitation of aged Cajanus cajan via modulating aquaporin, cell cycle regulatory genes and hormonal responses.

KEY MESSAGE: Nanoparticle pretreatment improved the health of aged Cajanus cajan seeds viz., regulation of redox status, gene expression, and restoration of hormonal homeostasis. Ageing deteriorates the quality of seeds by lowering their vigor and viability, and terminating with loss of germination. These days, nanotechnology has been seen to revolutionize the agricultural sectors, and particularly nano zinc oxide (nZnO) has gained considerable interests due to its distinctive properties. The aim of the present work was to decipher the possibilities of using nZnO to rejuvenate accelerated aged (AA) seeds of Cajanus cajan. Both chemically (CnZnO) and green (GnZnO; synthesized using Moringa oleifera) fabricated nZnOs were characterized via standard techniques to interpret their purity, size, and shape. Experimental results revealed erratic germination with a decline in viability and membrane stability as outcomes of reactive oxygen intermediate (ROI) buildup in AA seeds. Application of nZnO substantially rebated the accrual of ROI, along with enhanced production of antioxidants, α-amylase activity, total sugar, protein and DNA content. Higher level of zinc was assessed qualitatively/ histologically and quantitatively in nZnO pulsed AA seeds, supporting germination without inducing toxicity. Meantime, augmentation in the gibberellic acid with a simultaneous reduction in the abscisic acid level were noted in nZnO invigorated seeds than that determined in the AA seeds, suggesting possible involvement of ROI in hormonal signalling. Furthermore, nZnO-subjected AA seeds unveiled differential expression of aquaporins and cell cycle regulatory genes. Summarizing, among CnZnO and GnZnO, later one holds better potential for a revival of AA seeds of Cajanus cajan by providing considerable tolerance against ageing-associated deterioration via recouping the cellular redox homeostasis, hormonal signaling, and alteration in expression patterns of aquaporin and cell cycle regulatory genes.

Plant-Based Approaches for Rheumatoid Arthritis Regulation: Mechanistic Insights on Pathogenesis, Molecular Pathways, and Delivery Systems.

Rheumatoid arthritis (RA) is classified as a chronic inflammatory autoimmune disorder, associated with a varied range of immunological changes, synovial hyperplasia, cartilage destructions, as well as bone erosion. The infiltration of immune-modulatory cells and excessive release of proinflammatory chemokines, cytokines, and growth factors into the inflamed regions are key molecules involved in the progression of RA. Even though many conventional drugs are suggested by a medical practitioner such as DMARDs, NSAIDs, glucocorticoids, etc., to treat RA, but have allied with various side effects. Thus, alternative therapeutics in the form of herbal therapy or phytomedicine has been increasingly explored for this inflammatory disorder of joints. Herbal interventions contribute substantial therapeutic benefits including accessibility, less or no toxicity and affordability. But the major challenge with these natural actives is the need of a tailored approach for treating inflamed tissues by delivering these bioactive agentsat an appropriate dose within the treatment regimen for an extended periodof time. Drug incorporated with wide range of delivery systems such as liposomes, nanoparticles, polymeric micelles, and other nano-vehicles have been developed to achieve this goal. Thus, inclinations of modern treatment are persuaded on the way to herbal therapy or phytomedicines in combination with novel carriers is an alternative approach with less adverse effects. The present review further summarizes the significanceof use of phytocompounds, their target molecules/pathways and, toxicity and challenges associated with phytomolecule-based nanoformulations.

Mechanistic prospective and pharmacological attributes of quercetin in attenuation of different types of arthritis.

Arthritis is a frequent autoimmune disease with undefined etiology and pathogenesis. Scientific community constantly fascinating quercetin (QUR), as it is the best-known flavonoid among others for curative and preventive properties against a wide range of diseases. Due to its multifaceted activities, the implementation of QUR against various types of arthritis namely, rheumatoid arthritis (RA), osteoarthritis (OA), gouty arthritis (GA) and psoriotic arthritis (PsA) has greatly increased in recent years. Many research evidenced that QUR regulates a wide range of pathways for instance NF-κB, MAK, Wnt/ß-catenine, Notch, etc., that are majorly associated with the inflammatory mechanisms. Besides, the bioavailability of QUR is a major constrain to its therapeutic potential, and drug delivery techniques have experienced significant development to overcome the problem of its limited application. Hence, this review compiled the cutting-edge experiments on versatile effects of QUR on inflammatory diseases like RA, OA, GA and PsA, sources and bioavailability, therapeutic challenges, pharmacokinetics, clinical studies as well as toxicological impacts. The use of QUR in a health context would offer a tearing and potential therapeutic method, supporting the advancement of public health, particularly, of arthritic patients worldwide.

Allantoin: A Potential Compound for the Mitigation of Adverse Effects of Abiotic Stresses in Plants.

Stress-induced alterations vary with the species of plants, the intensity and duration of the exposure, and stressors availability in nature or soil. Purine catabolism acts as an inherent defensive mechanism against various abiotic stresses and plays a pivotal role in the stress acclimatisation of plants. The intermediate metabolite of purine catabolism, allantoin, compensates for soil nitrogen deficiency due to the low carbon/nitrogen ratio, thereby maintaining nitrogen homeostasis and supporting plant growth and development. Allantoin accounts for 90% of the total nitrogenous compound in legumes, while it contributes only 15% in non-leguminous plants. Moreover, studies on a variety of plant species have reported the differential accumulation of allantoin in response to abiotic stresses, endowing allantoin as a stress modulator. Allantoin functions as signalling molecule to stimulate stress-responsive genes (P5CS; pyrroline-5-carboxylase synthase) and ROS (reactive oxygen species) scavenging enzymes (antioxidant). Moreover, it regulates cross-talk between the abscisic acid and jasmonic acid pathway, and maintains ion homeostasis by increasing the accumulation of putrescine and/or spermine, consequently enhancing the tolerance against stress conditions. Further, key enzymes of purine catabolism (xanthine dehydrogenase and allantoinase) have also been explored by constructing various knockdown/knockout mutant lines to decipher their impact on ROS-mediated oxidative injury in plants. Thus, it is established that allantoin serves as a regulatory signalling metabolite in stress protection, and therefore a lower accumulation of allantoin also reduces plant stress tolerance mechanisms. This review gives an account of metabolic regulation and the possible contribution of allantoin as a photo protectant, osmoprotectant, and nitrogen recycler to reduce abiotic-stress-induced impacts on plants.

Contribution of strigolactone in plant physiology, hormonal interaction and abiotic stresses.

Strigolactones (SLs) are carotenoid-derived molecules, which regulate various developmental and adaptation processes in plants. These are engaged in different aspects of growth such as development of root, leaf senescence, shoot branching, etc. Plants grown under nutrient-deficient conditions enhance SL production that facilitates root architecture and symbiosis of arbuscular mycorrhizal fungi, as a result increases nutrient uptake. The crosstalk of SLs with other phytohormones such as auxin, abscisic acid, cytokinin and gibberellins, in response to abiotic stresses indicates that SLs actively contribute to the regulatory systems of plant stress adaptation. In response to different environmental circumstances such as salinity, drought, heat, cold, heavy metals and nutrient deprivation, these SLs get accumulated in plant tissues. Strigolactones regulate multiple hormonal responsive pathways, which aids plants to surmount stressful environmental constraints as well as reduce negative impact on overall productivity of crops. The external application of SL analog GR24 for its higher bioaccumulation can be one of the possible approaches for establishing various abiotic stress tolerances in plants.

Mechanisms underlying the phytotoxicity and genotoxicity of aluminum and their alleviation strategies: A review.

Aluminum (Al) is considered as a potential limiting factor for plant growth in acidic environment. At lower concentration, Al promotes plant growth by facilitating the phosphorous availability, while, at higher concentration, it causes rhizotoxicity by inhibiting the nutrient transportation system. Cellular membrane is identified as the first site of Al toxicity, which is consequent to Al-induced reactive oxygen species prompted lipid catabolism. Among all the soluble forms, the trivalent cationic form (Al3+) of Al is most toxic. Though, the ability to ascribe Al-tolerance is very complex, exclusion is an extensively established process contributing to Al3+ detoxification. Alteration in pH at root apex/rhizosphere, exudation of chelating agents, cell wall immobilization, and Al efflux have been recognized as probable methods for exclusion of Al, which is highly dependent on concentrations of organic acids, and plant species. Additionally, exogenous applications of boron, silicon, calcium, etc., in Al-stressed plant species can form a conjugate with it, thereby reducing its bioavailability/toxicity. Moreover, nanoparticles (NPs) are emerging tools in agricultural sector, which are found to be relatively more effective in mitigation of metal stress compared to their bulk materials. This review exhibits the fundamental approaches of Al phytotoxicity and endows with a comprehensive knowledge of the cellular and metabolic processes underlying toxic impacts along with ameliorative efficiencies of various potential agents including NPs. Additionally, it also elucidates the molecular mechanisms, future research prospects and challenges in effective alleviation mechanisms for enhancing plant Al-tolerance, to improve the growth and yields of susceptible-species on acidic soil.

Nanotechnology: an efficient approach for rejuvenation of aged seeds.

Modern agricultural efforts are now in search of an efficient, eco-friendly and sustainable approach for enhanced crop production. Nearly 50-60% of seeds lost occurs due to improper technical handling. Seed deterioration manifests itself as reduction in the rate of germination and growth with increased susceptibility to biotic and abiotic stresses. Furthermore, seed ageing is another economic and scientific issue that is associated with an array of internal (structural, physiological and genetic) and external (storage temperature and relative humidity) factors. Reactive oxygen species (ROS) are believed to be a key player in ageing phenomenon. However, hydrated storage, or ROS blockers are a few of the conventionally used methods to minimize the ageing process. Recently, exogenous applications of different inorganic nanoparticles (metal and metal oxide) are suggested to revitalize and revive aged seeds. Owing to their special properties of nano-size with high surface area they easily penetrate the seed coat. Exposure of nanoparticles has been suggested to neutralize the excess of ROS to a level that initiates hormonal signaling to support early emergence of radicles from the seeds. Nanotechnology has been well explored to enhance the crops nutritional quality, livestock productivity, plant protection from various stressors and in enhancement of seed quality via nanopesticides and nanofertilizers. Aiming at sustainable agriculture practices with fewer inputs, maximum benefits, ecologically safe and compatible technique the nanotechnology is an efficient approach to counteract problems of seed ageing incurring during storage, which is relatively less explored and unresolved conventionally, in general.

Carbon dot induces tolerance to arsenic by regulating arsenic uptake, reactive oxygen species detoxification and defense-related gene expression in Cicer arietinum L.

The scientific and technological applications of one of the nanomaterials viz.; carbon dot (C-dots), having extraordinary properties, is becoming an emerging and ongoing research area in recent times. In the present study, we have evaluated the effectiveness of C-dots in reducing arsenic (As) toxicity by analyzing physiological, biochemical and molecular parameters in Cicer arietinum L. The results revealed that As decreased the germination rate, growth, biomass, and membrane stability of the cell to a significant extent. Further, As was taken up by the growing seeds which eventually caused cell death. Levels of reactive oxygen species (ROS), stress markers (malondialdehyde), activities of defensive enzymes (glutathione-S-transferase and pyrroline-5-carboxylate synthetase) and non-enzymatic antioxidant contents (proline and glutathione) were increased under As stress. Moreover, As treatment resulted in the up-regulation of expressions of NADPH oxidase and defense-related genes in Cicer arietinum L. However, application of C-dots along with As improved the germination and growth of Cicer arietinum L. Exogenous application of C-dots, enhanced the expressions of defense-related genes and, contents of proline and glutathione, thereby causing considerable reductions in ROS, and malondialdehyde levels. Overall, this study suggests the possible involvement of C-dots in lowering the toxic effects of As on biomass by reducing As uptake and, inducing the activities/gene expressions and contents of enzymatic and non-enzymatic antioxidants.

Titanium nanoparticles attenuates arsenic toxicity by up-regulating expressions of defensive genes in Vigna radiata L.

Arsenic (As)-toxicity is recognized as one of the major environmental problems, affecting productivity of crops worldwide, thereby threatening sustainable agriculture and food security. Progression in nanotechnology and its impacts have brought up concerns about the application of engineered nanoparticles (NPs) in various sectors of the economy, including the field of agronomy. Among various NPs, there has been a rising amount of interest regarding the effects of titanium NPs (TiNPs) on plants growth and development, and their fate of abiotic stress tolerance. Hence, the present study was aimed to assess the ameliorative potentialities of chemically and biologically/green synthesized TiNPs to alleviate As-induced toxic responses in Vigna radiata L. The results revealed that exposure to As hindered the growth indices (radicle length and biomass) and membrane integrity, while were improved with the application of chemical and green synthesized TiNPs. In addition, treatment of As provoked the accretion of reactive oxygen species (superoxide and hydrogen peroxide) and malondialdehyde (a lipid peroxidized product), but were diminished by the supplementation of chemical and green manufactured TiNPs. The experimental data also signified that exogenous application of chemical and green synthesized TiNPs conferred tolerance to As-induced oxidative injuries via perking-up the expressions of antioxidant genes and enzyme systems viz; superoxide dismutase and catalase. Therefore, the present study inferred that chemically and green synthesized TiNPs, particularly green manufactured, effectively mitigated the adverse impacts of As by augmenting antioxidant machinery, thereby proving its potentiality in the alleviation of As-toxicity, at least in Vignaradiata L.

Characterization of arsenic resistant plant-growth promoting indigenous soil bacteria isolated from Center-East regions of India.

A total of 45 morphologically distinct arsenic (As)-resistant bacterial strains were isolated from the soils of different regions of Chhattisgarh, India. The minimum inhibitory concentration (MIC) values of these isolates varied widely in the range of 100-500 mM for arsenate [As(V)] and 15-30 mM for arsenite [As(III)]. Out of forty-five, three isolates viz; ARP3, ARRP3, and ADT5 also revealed plant growth-promoting properties, including phosphate solubilization and production of siderophores, indoleacetic acid, ammonia, and exopolysaccharide. Besides all these, the strains not only exhibited significant growth in the presence of As(V)/As(III) but also displayed higher efficiency (87%-94%) of As removal from the growth medium followed by intracellular accumulation (17-19 mg As/g). Hydride generation atomic absorption spectroscopic (HG-AAS) analysis revealed the intracellular accumulation of As, and the structural changes that took place in these isolates were further confirmed by microscopic studies. The 16 S rRNA and phylogenetic analyses unveiled that the isolates ARP3, ARRP3, and ADT5 belonged to genera Pseudomonas, Exiguobaterium, and Microbacterium, respectively. The conducted study suggested that such beneficial bacterial strains could be conveniently exploited at a commercial level for enhancing plant growth in As-contaminated agricultural fields, thereby improved productivity and enhanced bioremediation of soil having alarming strength of As.

Melatonin, glutathione and thiourea attenuates lead and acid rain-induced deleterious responses by regulating gene expression of antioxidants in Trigonella foenum graecum L.

Lead and acid rain are important abiotic stress factors that limit the growth, development, metabolic activity and yield of the crops. Melatonin (MT; an indoleamine molecule), glutathione (GSH; free thiol tripeptide) and thiourea (TU; non physiological thiol based ROS scavenger) have been known to mediate several physiological, biochemical and molecular processes in plants under different kinds of environmental threats. However, the roles of MT, GSH and TU in stress tolerance against combined effect of lead and simulated acid rain (SAR) remains inexpressible. In this study, we investigated the response of Trigonella foenum graecum L. (Fenugreek) to combined application of lead (1200 ppm) and SAR (pH 3.5), and the potential roles of MT (50 µM), GSH (1 mM) and TU (3 mM) in enhancing lead and SAR stress tolerance of Fenugreek. The results showed that co-application of each MT, GSH and TU along with lead and SAR improved the growth and development of seedlings. Moreover, MT, GSH and TU treatments stabilized the cell membrane integrity, reduced ROS accumulation [superoxide radical (O2-) and hydrogen peroxide (H2O2)], malondialdehyde (MDA) content, lipoxygenase (LOX) activity and, enhanced protein accumulation and up-regulated the gene expressions of catalase (CAT) and superoxide dismutase (SOD) significantly. Furthermore, the present work provides strong evidence regarding protective roles of MT, GSH and TU against oxidative stress resulted from lead and SAR stress in Fenugreek. Considering these observations, MT, GSH and TU can be utilized as efficient ROS scavengers, for improving growth and increasing antioxidant capacity in lead and SAR stressed seedlings.

Hydrolytic enzymes mediated lipid-DNA catabolism and altered gene expression of antioxidants under combined application of lead and simulated acid rain in Fenugreek (Trigonella foenum graecum L.) seedlings.

Understanding ill effects of simultaneous existence of various abiotic stresses, commonly observed due to various anthropogenic activities and global climate change these days, over plants growth, metabolic activity and yield responses are important for continued agricultural productivity and food security. In the present study, seedlings of Fenugreek (Trigonella foenum graecum L.) were subjected to lead (Pb, 1200 ppm) and/or simulated acid rain (SAR, pH 3.5) for 30 days, and were then analysed. The results revealed reduced growth, and total lipid and DNA contents, while enhanced Pb accumulation, biological concentration factor, biological accumulation coefficient, translocation factor, lipase activity, and levels of free fatty acid, conjugated diene, lipid hydroperoxide, DNA oxidation and DNase activity under Pb and/or SAR exposure. Additionally, activities and gene expression levels of antioxidants (superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase) were enhanced in response to applied treatments. The results also suggested that inhibitions/ accelerations determined under joint addition of Pb and SAR were comparatively more profound than those measured under their single application. Additionally, root was more sensitive to Pb treatment, compared to both leaf and shoot. Hence, under simultaneous presence of two or more number of abiotic stresses, the strategy opted by plants for survival is chiefly governed by the interaction between prevailing stressors, which is then conceived by plants as a new state of stress.

Desiccation-induced ROS accumulation and lipid catabolism in recalcitrant Madhuca latifolia seeds.

Loss of viability in desiccation-sensitive seeds of Madhuca latifolia (Roxb.) J. F. Macbr., an important multipurpose tropical tree, was correlated with seed water content (WC). WC declined from 0.59 to 0.19 g g-1 fresh mass, 35 days after harvest from mother plant, at ambient conditions (temperature 25 ± 2 °C, relative humidity 50 ± 2%). The desiccation-induced reduction in viability was related with an accumulation of reactive oxygen species (ROS) that promoted lipid peroxidation associated loss of membrane integrity. Conducted study revealed 1.6-19 folds rise in lipid peroxidized products in desiccated M. latifolia seeds, and was found to be linked inversely with WC and germination percentage. Additionally, increased activities (7 and 13 folds) of lipid hydrolyzing enzymes; lipase (EC 3.1.1.3) and lipoxygenase (EC 1.13.11.12) respectively, were discernible in desiccating M. latifolia seeds. In summary, increased ROS, lipid oxidation, lipase and lipoxygenase were strongly correlated with viability loss in desiccating M. latifolia seeds.

Imperative roles of salicylic acid and nitric oxide in improving salinity tolerance in Pisum sativum L.

This study was undertaken to scrutinize efficacy of salicylic acid (SA) and/or sodium nitroprusside [SNP, source of nitric oxide (NO)] to mitigate injury symptoms of saline stress in Pisum sativum L. Exposure to sodium chloride (NaCl) was found to be injurious to germinating P. sativum L. (var. Shubhra IM-9101) and a direct correlation between severity of toxicity and NaCl-concentrations could be discernible. Both SA and NO serves as signal molecules in plant stress responses, and play crucial roles in key regulatory pathways of growth, development and metabolism. The limiting effects of salinity on radicle length and biomass accumulation were considerably released by SA and/or SNP and among which their combined application was found to be the most promising. Supplemented SA and/or SNP, particularly their cocktail, resulted in a substantial decline in reactive oxygen species accumulation, which later caused reduced accumulations of malondialdehyde, 4-hydroxy-2-nonenal and protein carbonyl, in NaCl subjected germinating P. sativum L. seeds. SA and/or SNP had significant inducing effects on activities of superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase. Additionally, exogenous SA and/or SNP led to the higher proline, sugar and glycinebetaine contents, than that of the control. On the basis of accumulated results, it could be concluded that the cocktail of SA and SNP may be efficiently used to overcome the adverse signatures of salinity stress.

Arsenic-induced genotoxic responses and their amelioration by diphenylene iodonium, 24-epibrassinolide and proline in Glycine max L.

Presence of the toxic metalloid, "arsenic (As)" is ubiquitous in the environment especially in the soil and water. Its excess availability in the soil retards growth and metabolism of plants via (a) slowing down the cell division/elongation, (b) overproduction of reactive oxygen species (ROS), (c) modulation of antioxidant enzymes, and (d) alteration of DNA profile/genomic template stability (GTS). In the current study, diphenylene iodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) were used to analyze their roles in eliminating the adverse effects of As. Glycine max L. (variety JS 335) seeds were subjected to As (75 µM, Sodium arsenite was used as source of As), and in combination with DPI (10 µM), EBL (0.5 µM) or Pro (10 mM), for five consecutive days, and effects of these treatment combinations were analyzed on germination percentage, biomass, membrane stability, GTS and expressions of defensive genes. In addition, the levels of As, ROS, malondialdehyde, DNA content, oxidation, fragmentation, polymorphism, DNase activity, endogenous Pro and pyrroline-5-carboxylate synthetase activity were evaluated. The results indicated that the treatments of DPI, EBL or Pro are capable to alleviate detrimental effects of As, gauged from above variables, but with different magnitudes. Apropos As-stress mitigation, Pro was found to be the most effective under the confines of the study protocol. This study certainly provides new ideas for intensifying studies to unravel elusive central mechanism of amelioration involving use of DPI, EBL or Pro in plants with confirmed As-toxicity.

Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

Protein extraction for two-dimensional electrophoresis from tissues of recalcitrant species is quite problematic and challenging due to the low protein content and high abundance of contaminants. Proteomics in Shorea robusta is scarcely conducted due to the lack of a suitable protein preparation procedure. To establish an effective protein extraction protocol suitable for two-dimensional electrophoresis in Shorea robusta, four procedures (borate buffer/trichloroacetic acid extraction, organic solvent/trichloroacetic acid precipitation, sucrose/Tris/phenol, and organic solvent/phenol/sodium dodecyl sulfate) were evaluated. Following these, proteins were isolated from mature leaves and were analyzed for proteomics, and also for potential contaminants, widely reported to hinder proteomics. The borate buffer/trichloroacetic acid extraction had the lowest protein yield and did not result in any banding even in one-dimensional electrophoresis. In contrast, organic solvent/phenol/sodium dodecyl sulfate extraction allowed the highest protein yield. Moreover, during proteomics, organic solvent/phenol/sodium dodecyl sulfate extracted protein resolved the maximum number (144) of spots. Further, when proteins were evaluated for contaminants, significant (77-95%) reductions in the nucleic acids, phenol, and sugars were discernible with refinement in extraction procedure. Accumulated data suggested that the organic solvent/phenol/sodium dodecyl sulfate extraction was the most effective protocol for protein isolation for proteomics of Shorea robusta and can be used for plants that have a similar set of contaminants.

Increased rate of drying reduces metabolic inequity and critical water content in radicles of Cicer arietinum L.

Orthodox seed serves as easily accessible model to study desiccation-sensitivity in plant tissues because once they undergo germination, they become sensitive to desiccation imposed injuries. In the proposed study, effects of rate of drying on the viability, electrolyte leakage, superoxide accumulation, lipid-protein oxidation and antioxidant enzymes were explored in excised radicles of Cicer arietinum L. under dehydration and wet storage. For both the drying conditions, desiccation could be explained by exponential and inverse functions. Under rapid drying tissue viability as scored by germination efficiency and tetrazolium staining remained 100 % all through the analysis (24 h) but declined remarkably after 0.30 g g(-1) fresh mass water content (4 days) under slow drying. Moreover, precipitous fall in tissue viability was observed after 2 weeks of wet storage. Rapid drying was also accompanied with limited amounts of electrolyte leakage, superoxide radical, malondialdehyde and protein hydroperoxide, together with enhanced level of protein. Additionally, activities of both superoxide dismutase and ascorbate peroxidase were increased in rapidly dried radicles, but guaiacol peroxidase was declined. In contrary, above referred biomarkers were observed to perform either inversely or poorly during slow drying and wet storage suggesting that above documented alterations might be the resultant of ageing and not desiccation. Gathered data demonstrated that increased drying lowers the critical water content for tissue survival and also reduces the risk of damage resulting from aqueous-based deleterious reactions. Additionally, it also showed that growing radicles are a popular model to explore desiccation-sensitivity in plant tissues and/or seeds.

ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging.

Reactive oxygen species (ROS) and lipid peroxidation products appear to correlate strongly with the desiccation induced loss of viability in recalcitrant sal seeds. The 100% germination in fresh sal seeds declined with dehydration under natural storage conditions (26 ± 1 °C, relative humidity 52 ± 2%). Seeds became non-viable within 8 days. Desiccation induced disturbances in the metabolic activity of seeds resulted in generation of enormous amounts of ROS that are responsible for cellular damage and viability loss. Oxidative stress in the dehydrating aging sal seeds was further aggravated by inducing lipid peroxidation as the amounts of free fatty acid, conjugated diene, lipid hydroperoxide and secondary free radicals; malondialdehyde and 4-hydroxy-2-nonenal, were also promoted. In addition, significant rise in lipid degrading enzymes; lipase (EC 3.1.1.3) and lipoxygenase (LOX, EC 1.13.11.12) were detected in dehydrating sal seeds. Our results indicated multiple pathways (ROS, lipid peroxidation & lipase and LOX) that operate in the dehydrating recalcitrant sal seeds finally contributing to loss of viability.