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Izv Akad Nauk Ser Biol
; (5): 517-21, 2013.
Article
in Russian
| MEDLINE
| ID: mdl-25510103
ABSTRACT
Transgenic poultry with a gene of human granulocyte colony-stimulating factor (gcsf) was developed by arti- ficial insemination with the transfected sperm using the "Lipofectamin 2000" reagent. The ratio of trans- genic chickens carrying the human gcsf genome was 33.3% of the total number of birds obtained. It was shown that the concentration-of the human G-csfprotein in blood serum oftransgenic specimens varied from 50 to 220 pg/ml. The first generation of poultry was obtained as a result of insemination oftransgenic hens by sperm of anti-transgenic cocks, thereby inheritance of the foreign gene was found in 37.5% of the obtained chickens.