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1.
Appl Radiat Isot ; 57(2): 225-33, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12150282

ABSTRACT

Quantitative analysis of the two-dimensional image data obtained with the positron-emitting tracer imaging system (PETIS) for plant physiology has been carried out using a transfer function analysis method. While a cut leaf base of Chinese chive (Allium tuberosum Rottler) or a cut stem of soybean (Glycine max L.) was immersed in an aqueous solution containing the [18F] F- ion or [13N]NO3- ion, tracer images of the leaf of Chinese chive and the trifoliate of soybean were recorded with PETIS. From the time sequence of images, the tracer transfer function was estimated from which the speed of tracer transport and the fraction moved between specified image positions were deduced.


Subject(s)
Tomography, Emission-Computed/statistics & numerical data , Allium/physiology , Biological Transport, Active , Data Interpretation, Statistical , Fluorine Radioisotopes/pharmacokinetics , Image Processing, Computer-Assisted , Nitrogen Radioisotopes/pharmacokinetics , Plant Physiological Phenomena , Glycine max/physiology
2.
J Agric Food Chem ; 49(1): 276-80, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11305252

ABSTRACT

A simple and reliable method for the determination of NH4+, K+, Na+, aspartic acid, asparagine, glutamine, and alanine by ion chromatography has been developed. It is suitable for monitoring changes of nitrogen metabolism in soybean because it can accurately measure concentrations o asparagine and NH4+, two key substances for nitrogen storage and transport in this plant species Analysis of asparagine distribution in soybean indicated that higher levels (up to 18.4 micromol g(-1) of fresh mass) occur in stems and lower levels in roots (2.0 micromol g(-1) of fresh mass) and leaves (1.6 micromol g(-1) of fresh mass). When the herbicide metsulfuron-methyl (0.5, 5, and 50 ppb) was applied via the nutrient solution to the root system, asparagine concentrations increased 3-6 times in stems roots, and leaves. Metsulfuron-methyl is known to impair the synthesis of branched amino acids and, in consequence, protein synthesis. Thus, nitrogen consumption was limited, leading to ar accumulation of asparagine. The possible use of this physiological response in agricultural practice to identify herbicide stress in soybean and to detect low-level residues of sulfonylurea herbicides ir the soil is discussed.


Subject(s)
Amino Acids/analysis , Cations , Chromatography, Ion Exchange , Glycine max/drug effects , Glycine max/metabolism , Herbicides/pharmacology , Nitrogen/metabolism , Alanine/analysis , Arylsulfonates/pharmacology , Asparagine/analysis , Aspartic Acid/analysis , Glutamine/analysis , Plant Structures/chemistry , Potassium/analysis , Quaternary Ammonium Compounds/analysis , Sodium/analysis , Glycine max/chemistry , Tissue Distribution
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