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1.
Injury ; 50(11): 2093-2096, 2019 Nov.
Article in English | MEDLINE | ID: mdl-31383354

ABSTRACT

INTRODUCTION: Common practice in orthopedic surgery is to obtain postoperative radiographs to evaluate for healing or complications. Images obtained in the post-anesthesia care unit (PACU) have not been shown to positively impact patient care. This study plans to evaluate the clinical utility and cost-effectiveness of PACU postoperative radiographs following tibial plateau open reduction and internal fixation (ORIF). METHODS: Data from 211 patients who underwent a tibial plateau ORIF over a 5-year period at a single institution were retrospectively reviewed to determine if a patient received a postoperative radiograph in the PACU. Radiograph and clinical notes were reviewed to determine if postoperative radiograph resulted in management changes. Radiograph charges were calculated using CPT codes. RESULTS: A total of 142 of 211 patients (67.3%) who underwent tibial plateau ORIF received a postoperative radiograph while in the PACU. The majority of the radiographs had normal findings (88.7%). Of the 142 patients with postoperative imaging, subsequent management changes occurred for only one patient (0.7%). In this case, an incidental foot fracture was found which resulted in further CT imaging to assess the fracture. Other abnormal radiograph readings (11.3%) were generally due to incidental, chronic findings that did not require management changes. The average postoperative radiograph cost was $433.55 per patient, totaling $91,480 for 142 patients over a 5-year period. CONCLUSIONS: Routine postoperative radiographs following tibial plateau ORIF resulted in minimal management change patients in this series. The substantial cost of postoperative radiographs yielding little clinical utility suggests the use of routine PACU imaging following tibial plateau ORIF should be discontinued. Imaging would only be indicated in situations where intraoperative complications are suspected, thus reducing unnecessary imaging and patient cost.


Subject(s)
Knee Injuries/surgery , Knee Joint/diagnostic imaging , Tibial Fractures/surgery , Unnecessary Procedures , Adult , Continuity of Patient Care/economics , Female , Fracture Fixation, Internal , Health Care Costs , Humans , Knee Injuries/diagnostic imaging , Knee Injuries/physiopathology , Knee Joint/physiopathology , Knee Joint/surgery , Male , Middle Aged , Postoperative Period , Radiography/economics , Radiography/methods , Retrospective Studies , Tibial Fractures/diagnostic imaging , Tibial Fractures/physiopathology , Unnecessary Procedures/economics , Unnecessary Procedures/methods
2.
J Viral Hepat ; 20(3): 149-57, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23383653

ABSTRACT

Chronic hepatitis B and C virus infections are universally accepted as causes of hepatocellular carcinoma in humans. Hepatitis A and E viruses cause only acute self-limiting infections of the liver. Of the remaining hepatitis viruses - Delta hepatitis, hepatitis G (GB-C), TT and SEN - all have at some time been incriminated as causes of hepatocellular carcinoma. Delta hepatitis virus requires helper functions from hepatitis B virus to become invasive. Chronic Delta/hepatitis B viral co-infection runs a more severe course than that resulting from chronic hepatitis B virus infection alone, with progression to cirrhosis being more likely and more rapid. A substantial majority of the early studies did not find an increased incidence of hepatocellular carcinoma in co-infected individuals. But more recently, an increased incidence of the tumour has been recorded more often than no increase. Further studies are needed to draw a firm conclusion with regard to the hepatocarcinogenic effect of dual Delta/hepatitis B virus co-infection. With one exception, no published study (of 13) has incriminated chronic infection with hepatitis G virus as a cause of hepatocellular carcinoma. The dissenting study, published in 1999, was the only one performed in the United States. Fewer studies of the hepatocarcinogenic effect of TT virus have been performed. Apart from one study, published in 1999, no convincing evidence is available that supports a causal role for TT virus in hepatocarcinogenesis. The exception was in Japanese patients with high hepatitis C viral loads but independent of chronic hepatitis C virus infection. No evidence has been produced to indicate that SEN virus causes hepatocellular carcinoma.


Subject(s)
Carcinoma, Hepatocellular/virology , GB virus C/physiology , Hepatitis Delta Virus/physiology , Liver Neoplasms/virology , Torque teno virus/physiology , Animals , Humans
4.
S Afr Med J ; 102(3 Pt 1): 153-6, 2012 Feb 23.
Article in English | MEDLINE | ID: mdl-22380910

ABSTRACT

AIM: We aimed to ascertain if there had been any improvement in the number of nurses being immunised against hepatitis B virus (HBV) infection in a large academic hospital in which, 10 years previously, only 30.6% of the nurses were immune to infection with the virus, and to ascertain the incidence of infection with hepatitis C virus (HCV) and human immunodeficiency virus (HIV) in these nurses. METHODS: We studied 170 predominantly black nurses. Their blood was tested for the presence of active or past HBV infection using appropriate immunoassays, HCV infection by chromatographic immunoassays confirmed by polymerase chain reaction assays, and HIV using a rapid test confirmed by enzyme-linked immunosorbent assays. RESULTS: Serum of 89 (52.4%) nurses was positive for hepatitis B surface antibody (anti-HBs). Of these nurses 18 said that they had not received the vaccine; the serum of 9 of these was positive for anti-hepatitis B core antibody (anti-HBc) as well as anti-HBs, indicating natural infection with the virus. Of the nurses positive for anti-HBs, 89 were tested for anti-HBc; 28.2% tested positive for anti-HBc. Three nurses gave dates of immunisation that fell outside of their nursing careers; 3 (1.8%) were actively infected with the virus; 2 (1.8%) were infected with HCV; 10 nurses (5.9%) were positive for HIV. CONCLUSION: Nurses at this academic hospital remain at high risk of work-related HBV infection.


Subject(s)
Hepatitis B virus/immunology , Hepatitis B , Infectious Disease Transmission, Patient-to-Professional , Occupational Exposure/prevention & control , Vaccination , Adult , Female , HIV/immunology , HIV Infections/diagnosis , HIV Infections/epidemiology , HIV Infections/transmission , HIV Infections/virology , Hepacivirus/immunology , Hepatitis B/diagnosis , Hepatitis B/prevention & control , Hepatitis B/transmission , Hepatitis B/virology , Hepatitis B Core Antigens/blood , Hepatitis B Surface Antigens/blood , Hepatitis C/diagnosis , Hepatitis C/epidemiology , Hepatitis C/transmission , Hepatitis C/virology , Hospitals, University , Humans , Immunoassay/methods , Incidence , Infectious Disease Transmission, Patient-to-Professional/prevention & control , Infectious Disease Transmission, Patient-to-Professional/statistics & numerical data , Male , Middle Aged , Risk Assessment , Risk Reduction Behavior , South Africa/epidemiology , Vaccination/methods , Vaccination/statistics & numerical data
6.
S Afr J Surg ; 49(2): 68-74, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21614976

ABSTRACT

BACKGROUND: We describe the clinicopathologic features and outcome of South African patients who have undergone hepatic resection for hepatocellular carcinoma (HCC) arising in a non-cirrhotic liver. METHODS: We utilised the prospective liver resection database in the Surgical Gastroenterology Unit at Groote Schuur Hospital, Cape Town, to identify all patients who underwent surgery for HCC with non-cirrhotic liver parenchyma between 1990 and 2008. RESULTS: Twenty-two patients (10 men, 12 women, 3 black, 19 white, median age 47 years, range 21-79 years) underwent surgery for non-cirrhotic HCC. Sixteen patients had non-fibrolamellar HCC (Group 1); 6 patients had fibrolamellar HCC (Group 2). Group 1 had a median age of 55 years, and 6 (38%) were men; group 2 had a median age of 21 years, and 5 (83%) were men. Most patients had a solitary tumour at diagnosis; median largest tumour diameters in Groups 1 and 2 were 10 cm (range 4-21) and 12 cm (range 4-17), respectively. Patients in Group 1 underwent extended right hepatectomy (N=3), right hepatectomy (N=3), left hepatectomy (N=3), partial hepatectomy (N=7), cholecystectomy (N=6), and appendicectomy (N=1). Patients in Group 2 underwent extended right hepatectomy (N=1), right hepatectomy (N=1), left hepatectomy (N=2), segmentectomy (N=2), and portal lymphadenectomy (N=3). Recurrence rates in Groups 1, 2, and overall were 81%, 100% and 86%, respectively. Median overall survival was 46 months, with 1-, 3-, and 5-year survival rates of 95%, 59% and 45%, respectively. In Group 1, median survival was 39 months, with 1-, 3-, and 5-year survival rates of 100%, 56% and 38% respectively. In Group 2, median survival was 61 months, with 1-, 3-, and 5-year survival rates of 83%, 67% and 67%, respectively. CONCLUSION: Despite aggressive surgical resection, HCC arising in normal liver parenchyma has a high recurrence rate and an ultimately poor outcome. This finding is similar to both the recent international experience of non-cirrhotic HCC and local experience of fibrolamellar HCC.


Subject(s)
Carcinoma, Hepatocellular/surgery , Hepatectomy , Liver Neoplasms/surgery , Adult , Aged , Carcinoma, Hepatocellular/mortality , Female , Hepatectomy/methods , Humans , Liver Neoplasms/mortality , Male , Middle Aged , Patient Selection , South Africa , Young Adult
7.
Pathol Biol (Paris) ; 58(4): 273-7, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20378277

ABSTRACT

Approximately 360 million people worldwide are chronically infected with hepatitis B virus (HBV) and are at high risk of developing hepatocellular carcinoma (HCC). Chronic HBV infection is the most prevalent cause of this tumour, accounting for 55% of global cases, and 89% of those in endemic regions for HBV infection. Relative risks for developing HCC in the presence of chronic HBV infection may be as high as 49 in case-control studies, and 98 in cohort studies. HCC is the sixth most common cancer in the world today, with approximately 630,000 new cases occurring each year. It ranks third in annual cancer mortality rates. Approximately 80% of HCCs occur in developing countries where HBV infection is endemic, with the highest incidences being in the Asia-Pacific region, and sub-Saharan Africa. In the chronic carriers of the virus who are at greatest risk of developing HCC, the infection is acquired at birth or in the early months or years of life, either perinatally or horizontally, and frequently becomes chronic. The risks are greater in males, and older individuals, and are increased by co-exposure to aflatoxin B(1), the presence of cirrhosis, obesity, or diabetes mellitus, and possibly co-infection with hepatitis C virus. Viral factors that influence the risk of HCC are high viral load, the presence of certain mutations, and genotypes. Although the incidence of chronic HBV infection is beginning to decrease as a result of the universal infant immunization programme, HBV-induced HCC incidence is projected to increase for at least another two decades.


Subject(s)
Carcinoma, Hepatocellular/epidemiology , Carcinoma, Hepatocellular/virology , Hepatitis B, Chronic/epidemiology , Liver Neoplasms/epidemiology , Liver Neoplasms/virology , Aflatoxin B1/toxicity , Carcinoma, Hepatocellular/chemically induced , Endemic Diseases , Female , Hepatitis B Vaccines , Hepatitis B, Chronic/complications , Hepatitis B, Chronic/prevention & control , Humans , Liver Neoplasms/chemically induced , Male , Risk , Sex Factors
8.
Article in English | MEDLINE | ID: mdl-19782547

ABSTRACT

Altered membrane integrity in hepatocellular carcinoma (HCC) tissue was indicated by an elevation in cholesterol and significant decrease in phosphatidylcholine (PC). The resultant decreased phosphatidylcholine/phosphatidylethanolamine (PC/PE) and increased cholesterol/phospholipid ratios are associated with decreased fluidity in the carcinoma tissue. The lower PC was associated with a decrease in the quantitative levels of the saturated (C16:0, C18:0), omega6 (C18:2, C20:4) and omega3 (C22:5, C22:6) fatty acids (FAs), resulting in reduced long-chain polyunsaturated fatty acids (LCPUFAs), total PUFA and an increase in omega6/omega3 FA ratio. In PE, the saturated and omega3 (C22:5, C22:6) FAs were reduced while the total omega6 FA level was not affected, leading to an increased omega6/omega3 FA ratio. Increased levels of C18:1omega9, C20:2omega6 and reduction of 22:6omega3 in PC and PE suggest a dysfunctional delta-6 desaturase. The reduced PC/PE ratio resulted in a decreased C20:4omega6 (PC/PE) ratio, implying a shift towards synthesis of the 2-series eicosanoids. Lipid peroxidation was reduced in both hepatitis B negative (HBV(-)) and positive (HBV(+)) HCC tissues. Glutathione (GSH) was decreased in HCC while HBV had no effect, suggesting an impairment of the GSH redox cycle. In contrast HBV infection enhanced GSH in the surrounding tissue possibly to counter oxidative stress as indicated by the increased level of conjugated dienes. Apart from the reduced LCPUFA, the low level of lipid peroxidation in the carcinoma tissue was associated with increased superoxide dismutase and glutathione peroxidase activity. The disruption of the redox balance, resulting in increased cellular antioxidant capacity, could create an environment for resistance to oxidative stress in the carcinoma tissue. Alterations in membrane cholesterol, phospholipids, FA parameters, C20:4omega6 membrane distribution and low lipid peroxidation are likely to be important determinants underlying the selective growth advantage of HCC cells.


Subject(s)
Carcinoma, Hepatocellular/metabolism , Hepatitis B virus/physiology , Lipids/analysis , Liver Neoplasms/metabolism , Oxidative Stress/physiology , Adiposity/physiology , Carcinoma, Hepatocellular/pathology , Carcinoma, Hepatocellular/virology , Case-Control Studies , Catalase/analysis , Glutathione/analysis , Glutathione/metabolism , Glutathione Disulfide/analysis , Glutathione Disulfide/metabolism , Glutathione Peroxidase/analysis , Glutathione Peroxidase/metabolism , Health Status , Hepatitis B virus/isolation & purification , Humans , Lipid Metabolism/physiology , Liver/chemistry , Liver/pathology , Liver/virology , Liver Neoplasms/pathology , Liver Neoplasms/virology , Superoxide Dismutase/analysis , Superoxide Dismutase/metabolism
9.
Article in English | AIM (Africa) | ID: biblio-1270591

ABSTRACT

Hepatitis B virus infection; both acute and chronic; occurs commonly in the black population of South Africa; and chronic infection and its sequelae of cirrhosis and hepatocellular carcinoma are major public health threats. Chronic hepatitis B virus infection is rare in the other population groups; with the exception of the very small Chinese community. Prevalences of chronic carriage of hepatitis B virus in South African blacks are 5-16in rural males; 8-9in urban males; 4-12in rural females; and 2.7-4in urban females. The overall male to female ratio is 2.6:1.0. There are now three to four million South African blacks who are chronically infected with this virus. In rural black populations chronic hepatitis B virus infection is acquired very early in life; predominantly as a result of horizontal transmission of the virus; and by the age of 5 years carrier rates approach those seen in adulthood. A further slight increase occurs at school-going age and a greater increase at the time of becoming sexually active. Urban black carrier rates are significantly lower and the infection is acquired later in life. The decreased urban viral carriage rates occur mainly in the first generation born in an urban environment. Hepatitis B virus accounts for about 60of clinically evident acute viral hepatitis among blacks and about 10of chronic hepatitis and cirrhosis. It is the cause of the majority of the many cases of hepatocellular carcinoma that occur in black South Africans. The tumour is more common in males and in rural-born than in urban-born blacks. The close association between chronic hepatitis B virus infection and hepatocellular carcinoma holds true in rural and urban patients and males and females. The association is age-related; being closer in younger patients. Genotypes A and D of hepatitis B virus predominate in South African isolates; with genotype A and its subtype Aa having aparticularly high hepatocarcinogenic potential


Subject(s)
Carcinoma , Fibrosis , Hepatitis B virus/diagnosis , Hepatitis B virus/epidemiology
10.
J Viral Hepat ; 13(3): 145-9, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16475989

ABSTRACT

Although hepatitis B (HBV) and C viruses (HCV) are, individually, major causes of hepatocellular carcinoma, the interaction, if any, between the carcinogenic effects of the two viruses is uncertain. Equal numbers of published studies have reported no risk interaction or a synergistic risk interaction. These conflicting results are explained by the rarity of concurrent infection with HBV and HCV in individuals without clinically evident liver disease, which severely limits the ability to accurately estimate the hepatocarcinogenic risk of dual infection compared with that of either infection alone. In an attempt to circumvent this difficulty, two meta-analyses have been performed, one based on studies published from a number of countries and the other on studies confined to Chinese patients. Both analyses concluded that a synergistic carcinogenic interaction existed between the two viruses and that the increased risk was super-additive but not multiplicative. If confirmed, this risk interaction will occur against a background of negative confounding effects on viral replication between HBV and HCV, which may be reciprocal. The mechanisms responsible for the carcinogenic interaction between the viruses are unknown. One possibility is that the increased incidence of cirrhosis with concurrent HBV and HCV infections acts as an even more potent tumour promoter than occurs with either virus alone. Synergism between the direct hepatocarcinogenic effects of the two viruses is another possible mechanism, but proof will have to await a fuller understanding of the pathogenetic mechanisms involved with the individual viruses.


Subject(s)
Carcinoma, Hepatocellular/virology , Hepacivirus/physiology , Hepatitis B virus/physiology , Hepatitis B/complications , Hepatitis C/complications , Liver Neoplasms/virology , Hepatitis B/virology , Hepatitis C/virology , Humans
11.
Liver Int ; 26(1): 1-7, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16420504

ABSTRACT

Although rare in most countries, membranous obstruction of the inferior vena cava (MOIVC) occurs more frequently in Nepal, South Africa, Japan, India, China, and Korea. The occlusive lesion always occurs at approximately the level of the diaphragm. It commonly takes the form of a membrane, but may be a fibrotic occlusion of variable length. Controversy exists as to whether MOIVC is a developmental abnormality or a result of organization of a thrombus in the hepatic portion of the inferior vena cava. The outstanding physical sign associated with MOIVC are large truncal collateral vessels with a cephalad flow. A dilated vena azygous is seen on chest radiography. Definitive diagnosis is made by contrast inferior vena cavography. The long-standing obstruction to hepatic venous flow causes severe centrolobular fibrosis and predisposes to the development of hepatocellular carcinoma (HCC). Percutaneous balloon angioplasty, transatrial membranotomy, or more complex vena caval and portal decompression surgery should be performed to prevent these complications. HCC occurs in more than 40% of South African Black and Japanese patients with MOIVC, but less often in other populations. It is thought to result from the tumour-promoting effect of continuous hepatocyte necrosis, although the associated environmental risk factors have not been identified.


Subject(s)
Carcinoma, Hepatocellular/etiology , Liver Neoplasms/etiology , Vena Cava, Inferior/abnormalities , Venous Thrombosis/complications , Venous Thrombosis/diagnosis , Adult , Age Distribution , Aged , Angioplasty, Balloon/methods , Arteriovenous Malformations/diagnostic imaging , Arteriovenous Malformations/ethnology , Biopsy, Needle , Carcinoma, Hepatocellular/epidemiology , Carcinoma, Hepatocellular/pathology , Carcinoma, Hepatocellular/surgery , Female , Humans , Immunohistochemistry , Incidence , Liver Circulation , Liver Neoplasms/epidemiology , Liver Neoplasms/pathology , Liver Neoplasms/surgery , Male , Middle Aged , Phlebography , Risk Assessment , Severity of Illness Index , Sex Distribution , South Africa/epidemiology , Stents , Survival Rate , Thrombolytic Therapy/methods , Venous Thrombosis/therapy
12.
Oncogene ; 25(7): 1008-17, 2006 Feb 16.
Article in English | MEDLINE | ID: mdl-16247464

ABSTRACT

Hepatitis B virus (HBV)-encoded X antigen (HBxAg) contributes to the development of hepatocellular carcinoma (HCC). A frequent characteristic of HCC is reduced or absent expression of the cell adhesion protein, E-cadherin, although it is not known whether HBxAg plays a role. To address this, the levels of E-cadherin were determined in HBxAg-positive and -negative HepG2 cells in culture, and in tumor and surrounding nontumor liver from a panel of HBV carriers. The results showed an inverse relationship between HBxAg and E-cadherin expression both in tissue culture and in vivo. In HBxAg-positive cells, E-cadherin was suppressed at both the mRNA and protein levels. This was associated with hypermethylation of the E-cadherin promoter. Depressed E-cadherin correlated with HBxAg trans-activation function, as did the migration of HepG2 cells in vitro. Decreased expression of E-cadherin was also associated with the accumulation of beta-catenin in the cytoplasm and/or nuclei in tissues and cell lines, which is characteristic of activated beta-catenin. Additional work showed that HBxAg-activated beta-catenin. Together, these results suggest that the HBxAg is associated with decreased expression of E-cadherin, accumulation of beta-catenin in the cytoplasm and nucleus, and increased cell migration, which may contribute importantly to hepatocarcinogenesis.


Subject(s)
Cadherins/metabolism , Carcinoma, Hepatocellular/virology , Liver Neoplasms/virology , Trans-Activators/metabolism , Cadherins/genetics , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/metabolism , Cell Movement , Cell Nucleus/chemistry , Cytoplasm/chemistry , DNA Methylation , Down-Regulation , Hepatitis B virus , Humans , Liver Neoplasms/genetics , Liver Neoplasms/metabolism , Promoter Regions, Genetic , Protein Transport , Trans-Activators/analysis , Trans-Activators/genetics , Transfection , Viral Regulatory and Accessory Proteins , beta Catenin/analysis , beta Catenin/metabolism
13.
J Viral Hepat ; 12(5): 456-64, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16108759

ABSTRACT

SUMMARY: Phylogenetic analysis has led to the classification of hepatitis B virus into eight genotypes, designated A to H. The genotypes have differences in biological properties and show heterogeneity in their global distribution. These attributes of the genotypes may account not only for differences in the prevalence of hepatitis B virus mutants in various geographic regions, but also be responsible for differences in the clinical outcome and response to antiviral treatment in different population groups.


Subject(s)
Antiviral Agents/administration & dosage , DNA, Viral/genetics , Genetic Heterogeneity , Hepatitis B virus/genetics , Hepatitis B/genetics , Antiviral Agents/pharmacology , Carcinoma, Hepatocellular/virology , Disease Progression , Genotype , Hepatitis B/drug therapy , Hepatitis B/pathology , Hepatitis B virus/drug effects , Hepatitis B virus/pathogenicity , Humans , Mutation , Prevalence , Prognosis
14.
Liver Int ; 25(2): 201-13, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15780040

ABSTRACT

A growing body of evidence indicates that human immunodeficiency virus (HIV)-positive individuals are more likely to be infected with hepatitis B virus (HBV) than HIV-negative individuals, possibly as a result of shared risk factors. There is also evidence that HIV-positive individuals who are subsequently infected with HBV are more likely to become HBV chronic carriers, have a high HBV replication rate, and remain hepatitis Be antigen positive for a much longer period. In addition, it is evident that immunosuppression brought about by HIV infection may cause reactivation or reinfection in those previously exposed to HBV. Furthermore, HIV infection exacerbates liver disease in HBV co-infected individuals, and there is an even greater risk of liver disease when HIV and HBV co-infected patients are treated with highly active anti-retroviral therapy (HAART). Complicating matters further, there have been several reports linking HIV infection to 'sero-silent' HBV infections, which presents serious problems for diagnosis, prevention, and control. In sub-Saharan Africa, where both HIV and HBV are endemic, little is known about the burden of co-infection and the interaction between these two viruses. This paper reviews studies that have investigated HIV and HBV co-infection in sub-Saharan Africa, against a backdrop of what is currently known about the interactions between these two viruses.


Subject(s)
AIDS-Related Opportunistic Infections/epidemiology , AIDS-Related Opportunistic Infections/prevention & control , Communicable Disease Control/organization & administration , Hepatitis B/epidemiology , Hepatitis B/prevention & control , AIDS-Related Opportunistic Infections/drug therapy , Africa South of the Sahara/epidemiology , Antiretroviral Therapy, Highly Active/methods , Antiviral Agents/therapeutic use , Comorbidity , Developing Countries , Female , Hepatitis B/drug therapy , Hepatitis B Vaccines/administration & dosage , Hepatitis B virus/isolation & purification , Humans , Male , Prevalence , Program Development , Program Evaluation , Risk Assessment , Severity of Illness Index , Sex Distribution , Vaccination/methods
15.
J Viral Hepat ; 11(3): 198-205, 2004 May.
Article in English | MEDLINE | ID: mdl-15117321

ABSTRACT

In spite of advances made in our understanding of the biology of the hepatitis C virus (HCV), the epidemiology and natural history of HCV infection, and the treatment of chronic hepatitis C, the development and worldwide implementation of a comprehensive prevention and control strategy remains necessary. A World Health Organization informal consultation with the Viral Hepatitis Prevention Board was convened and met in Geneva, Switzerland, 13-14 May 2002, to review epidemiological and public health aspects of HCV infection, and the various prevention and control strategies that are currently in place. Based on the presentations and discussions, a number of specific recommendations were made, which should be considered in conjunction with previously published recommendations.


Subject(s)
Hepatitis C/prevention & control , Antiviral Agents/therapeutic use , Female , Health Education , Hepatitis C/drug therapy , Hepatitis C/etiology , Hepatitis C/transmission , Humans , Male , Risk-Taking
16.
J Med Virol ; 66(4): 468-71, 2002 Apr.
Article in English | MEDLINE | ID: mdl-11857523

ABSTRACT

The main reason to ascertain whether baboons are susceptible to infection with hepatitis C virus (HCV) is the need to replace chimpanzees, which are endangered, as an animal model for undertaking research into the biology and host-virus interactions of HCV, and for developing a vaccine against this virus. A second reason is that baboons are a possible source of xenografts for human liver transplantation. We inoculated serum containing HCV into four Chacma baboons and monitored them for 52 weeks for evidence of infection. Serum was tested for antibody to HCV, HCV RNA, and aminotransferase concentrations at 2-week intervals for 26 weeks and thereafter at 4-week intervals. Liver tissue was examined at 28 and 52 weeks for histopathological changes and viral RNA, and at 52 weeks for viral particles using electron microscopy. Reverse transcription-polymerase chain reaction assay was used to detect HCV RNA, and the results were confirmed by Southern hybridization. Serum aminotransferase concentrations remained within the normal range and liver histology was normal during the follow-up period. Passive transmission of anti-HCV to the baboons was observed during the first 4 weeks. HCV RNA was not detectable in any serum or liver sample and electron microscopy failed to reveal viral particles in liver tissue. In conclusion, we did not find Chacma baboons to be susceptible to infection with HCV, although we cannot deny that in an immunosuppressed liver transplant recipient, infection of a baboon xenograft might occur. Another animal model for HCV infection must be sought.


Subject(s)
Disease Models, Animal , Hepacivirus/pathogenicity , Hepatitis C/physiopathology , Papio , Animals , Female , Hepatitis C/pathology , Hepatitis C/virology , Humans , Liver/pathology , Liver/virology , Transplantation, Heterologous
17.
Cancer Res ; 61(21): 7803-10, 2001 Nov 01.
Article in English | MEDLINE | ID: mdl-11691796

ABSTRACT

The hepatitis B virus (HBV) X protein (HBx) is a transcriptional transactivator that has been implicated in the development of HBV-related hepatocellular carcinoma. Mutations in the HBx open reading frame have been reported, but their general impact on the biological function of HBx remains unknown. To address this issue, we comparatively analyzed the structures and biological functions of HBx sequences isolated from sera and from tumor and nontumor tissues of patients with a HBV-related hepatocellular carcinoma. In addition to the HBx sequences derived from free HBV genomes, HBx from HBV integrants was also obtained from the tumor tissues by use of a HBx-Alu PCR-based approach. Sequence analysis showed that the HBx sequences derived from tumor tissues (6 of 7), particularly those isolated from HBV integrants (4 of 4), contained a deletion in the distal COOH-terminal region. Interestingly, most of the COOH-terminally truncated HBx sequences obtained from tumor tissues, in contrast to the full-length HBx isolated from the sera and nontumor tissues, lost their transcriptional activity and their inhibitory effects on cell proliferation and transformation. Importantly, although full-length HBx suppressed the focus formation induced by the cooperation of ras and myc oncogenes in primary rat embryo fibroblasts, COOH-terminally truncated HBx enhanced the transforming ability of ras and myc. Finally, by analyzing the artificial mutants, we were able to more precisely map the functional domains located at the COOH-terminal of HBx. Taken together, our results suggest a key role for the HBx COOH-terminal end in controlling cell proliferation, viability, and transformation. This study further supports the hypothesis that natural HBx mutants might be selected in tumor tissues and play a role in hepatocarcinogenesis by modifying the biological functions of HBx.


Subject(s)
Carcinoma, Hepatocellular/virology , Hepatitis B virus/genetics , Liver Neoplasms/virology , Mutation , Trans-Activators/physiology , Amino Acid Sequence , Apoptosis/physiology , Carcinoma, Hepatocellular/chemistry , Carcinoma, Hepatocellular/genetics , Genes, myc/physiology , Genes, ras/physiology , Growth Inhibitors/genetics , Growth Inhibitors/physiology , Humans , Liver Neoplasms/chemistry , Liver Neoplasms/genetics , Molecular Sequence Data , Protein Structure, Tertiary , Sequence Homology, Amino Acid , Trans-Activators/genetics , Trans-Activators/isolation & purification , Transcriptional Activation , Transfection , Viral Regulatory and Accessory Proteins
18.
Dig Dis Sci ; 46(10): 2173-8, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11680593

ABSTRACT

Since oxidative DNA damage plays a role in experimental carcinogen-induced cancers, the purpose of the present study was to determine if hepatic oxidative DNA damage was increased in patients with HCC compared to patients with benign hepatic tumors or hepatic metastases (non-HCC) or to patients with end-stage alcoholic liver disease undergoing liver transplantation. Oxidative DNA damage was assessed by 8-hydroxy-2'-deoxyguanosine (8-OH-dG). Results showed that peritumoral 8-OH-dG was markedly increased in HCC (N= 51) (180 +/- 74 vs 32 +/- 58-OH-dG/10(6)dG for tumor, P < 0.005) in contrast to patients with non-HCC (N = 17), in whom the peritumoral 8-OH-dG did not differ from that in tumor (39 +/- 7 vs. 31 +/- 108-OH-dG/10(6)dG). Oxidative DNA damage can be both mutagenic and carcinogenic; our data suggested it will be important in future studies to determine the chronology of this type of liver injury relative to hepatocarcinogenesis.


Subject(s)
Carcinoma, Hepatocellular/genetics , DNA Damage , Deoxyguanosine/analogs & derivatives , Liver Neoplasms/genetics , Liver/metabolism , 8-Hydroxy-2'-Deoxyguanosine , Adult , Carcinoma, Hepatocellular/metabolism , Humans , Liver Diseases, Alcoholic/metabolism , Liver Neoplasms/metabolism
19.
J Med Virol ; 65(3): 485-92, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11596083

ABSTRACT

To investigate further the possible role of mutant hepatitis B viruses in the pathogenesis of fulminant hepatitis B, the genomic sequence of hepatitis B virus isolates from 9 South African blacks with this disease, including 5 entire genomes, was analysed. Seven of the isolates were genotype A. The mutation most often reported in patients with fulminant hepatitis B, the G1896A precore stop-codon substitution, was, as expected, not present in the genotype A isolates with the exception of one in which it was accompanied by a compensatory C1858T substitution. G1896A was, however, present in the one genotype D isolate. No other precore-defective mutants were detected. The other mutation commonly found in patients with fulminant hepatitis B, the paired A1762T, G1764A substitution in the basic core promoter, was present in only one patient and G1764A in one other. The pre-surface initiation-codon mutation documented in a number of patients with fulminant hepatitis B was not found in our isolates. An 18-amino acid deletion present in the pre-surface region of one isolate has not previously been described in fulminant hepatitis B. Variations within the surface region were mainly genotype specific and not previously described. A relatively large number of mutations were present in the middle region of the core gene in those isolates without G1896A or A1762T, G1764A mutations, although the pattern was not consistent with those in published studies. Thus, as in other published series in which the entire genome of hepatitis B virus responsible for fulminant hepatitis was sequenced, we detected many mutations in different genes, but none was common to all the reported isolates.


Subject(s)
Black People , Genome, Viral , Hepatitis B virus/genetics , Hepatitis B/virology , Sequence Analysis, DNA , Adolescent , Adult , Amino Acid Substitution , Child , Female , Hepatitis B/epidemiology , Hepatitis B virus/classification , Hepatitis B virus/isolation & purification , Humans , Male , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction
20.
Eur J Gastroenterol Hepatol ; 13(7): 865-7, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11474318

ABSTRACT

We describe three black South African patients in whom hepatocellular carcinoma metastasized to the umbilicus. Sister Joseph's nodule has previously been reported in only two patients with this tumour. A number of routes for this spread are possible: malignant hepatocytes in the portal venous system may reach the umbilicus via a patent umbilical vein; the tumour may propagate directly along the ligamentum teres hepatis to the umbilicus; contiguous spread of the tumour to the umbilicus from anterior peritoneal tissue, either directly infiltrated by hepatocellular carcinoma or the site of metastatic nodules, may occur; embolization of malignant hepatocytes to the umbilicus might take place by way of its arterial blood supply; or hepatocellular carcinoma might reach the umbilicus as a result of retrograde lymphatic spread from para-aortic lymph nodes or from the anterior abdominal wall, to which the tumour has metastasized.


Subject(s)
Abdominal Neoplasms/secondary , Carcinoma, Hepatocellular/secondary , Liver Neoplasms/pathology , Umbilicus , Abdominal Neoplasms/diagnosis , Adult , Carcinoma, Hepatocellular/diagnosis , Female , Humans , Liver Neoplasms/diagnosis , Male , Middle Aged
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