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1.
PLoS One ; 11(7): e0159371, 2016.
Article in English | MEDLINE | ID: mdl-27438012

ABSTRACT

Genetic variation has been observed in both protein concentration in wheat grain and total protein content (protein yield). Here we describe the genetic analysis of variation for grain protein in response to nitrogen (N) supply and locate significant genomic regions controlling grain protein components in a spring wheat population. In total, six N use efficiency (NUE) field trials were carried out for the target traits in a sub-population of doubled haploid lines derived from a cross between two Australian varieties, RAC875 and Kukri, in Southern and Western Australia from 2011 to 2013. Twenty-four putative Quantitative Trait Loci (QTL) for protein-related traits were identified at high and low N supply and ten QTL were identified for the response to N for the traits studied. These loci accounted for a significant proportion of the overall effect of N supply. Several of the regions were co-localised with grain yield QTL and are promising targets for further investigation and selection in breeding programs.


Subject(s)
Chromosome Mapping , Nitrogen/pharmacology , Seeds/genetics , Triticum/genetics , Australia , Climate , Crosses, Genetic , Edible Grain/genetics , Haploidy , Phenotype , Plant Proteins/metabolism , Quantitative Trait Loci/genetics , Quantitative Trait, Heritable , Seeds/drug effects , Soil/chemistry , Triticum/drug effects
2.
PLoS One ; 11(7): e0159374, 2016.
Article in English | MEDLINE | ID: mdl-27459317

ABSTRACT

Nitrogen (N) is a major nutrient needed to attain optimal grain yield (GY) in all environments. Nitrogen fertilisers represent a significant production cost, in both monetary and environmental terms. Developing genotypes capable of taking up N early during development while limiting biomass production after establishment and showing high N-use efficiency (NUE) would be economically beneficial. Genetic variation in NUE has been shown previously. Here we describe the genetic characterisation of NUE and identify genetic loci underlying N response under different N fertiliser regimes in a bread wheat population of doubled-haploid lines derived from a cross between two Australian genotypes (RAC875 × Kukri) bred for a similar production environment. NUE field trials were carried out at four sites in South Australia and two in Western Australia across three seasons. There was genotype-by-environment-by-treatment interaction across the sites and also good transgressive segregation for yield under different N supply in the population. We detected some significant Quantitative Trait Loci (QTL) associated with NUE and N response at different rates of N application across the sites and years. It was also possible to identify lines showing positive N response based on the rankings of their Best Linear Unbiased Predictions (BLUPs) within a trial. Dissecting the complexity of the N effect on yield through QTL analysis is a key step towards elucidating the molecular and physiological basis of NUE in wheat.


Subject(s)
Genetic Association Studies , Nitrogen/metabolism , Quantitative Trait Loci , Quantitative Trait, Heritable , Triticum/genetics , Triticum/metabolism , Biomass , Breeding , Chromosome Mapping , Crosses, Genetic , Edible Grain , Environment , Fertilizers , Genetic Linkage , Genotype , Triticum/growth & development
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