ABSTRACT
Ectonucleotidases play an important role in regulating the level of extracellular nucleotides and nucleosides and are an important part of the regulation of the effects of adenosine and ATP on adenosine and P2 receptors, respectively. We have previously established the ambiguous effect of P2 receptor agonists on the contractile activity of smooth muscle tissue in rats with the valproate model of autism. In this work, HPLC was used to evaluate the activity of ectonucleotidases in the smooth muscle tissues of the internal organs of rats with a valproate model of autism. The activity of ectonucleotidases was significantly higher in the smooth muscle tissues of the duodenum, vas deferens, and bladder, but lower in the ileum and uterus. The results obtained make it possible to compare the activity of ectonucleotidases identified here with changes in P2 receptor-mediated contractility of smooth muscle tissues revealed in our previous experiments.
Subject(s)
Autistic Disorder , Muscle Contraction , Muscle, Smooth , Urinary Bladder , Valproic Acid , Vas Deferens , Animals , Rats , Muscle, Smooth/drug effects , Muscle, Smooth/metabolism , Valproic Acid/pharmacology , Autistic Disorder/metabolism , Autistic Disorder/chemically induced , Autistic Disorder/drug therapy , Male , Female , Vas Deferens/drug effects , Vas Deferens/metabolism , Urinary Bladder/drug effects , Urinary Bladder/metabolism , Urinary Bladder/enzymology , Muscle Contraction/drug effects , Uterus/drug effects , Uterus/metabolism , Ileum/drug effects , Ileum/metabolism , Ileum/enzymology , Disease Models, Animal , Rats, Wistar , Receptors, Purinergic P2/metabolism , Adenosine Triphosphatases/metabolismSubject(s)
Back Pain/therapy , Exercise Therapy , Lumbar Vertebrae , Muscle Denervation , Muscular Dystrophies/therapy , Osteochondritis/complications , Adolescent , Adult , Back Pain/etiology , Combined Modality Therapy , Humans , Isometric Contraction , Male , Middle Aged , Muscular Dystrophies/etiologyABSTRACT
The blood serum lactate dehydrogenase (LDH), creatine kinase (CK), and LDH isoforms activities have been measured in 103 patients with the compression neural and myodystrophic syndromes of lumbar osteochondrosis. The studies have revealed a relationship between the myodystrophic process severity and the intensity of LDH and CK ejection from the muscle into the blood, particularly in the trigger stage. The shift of LDH activity towards the predominance of slowly migrating isoforms augments and the total blood serum LDH and CK activities grow as the muscular syndrome progresses. These data permit a differentiation between the compression neural and the myodystrophic syndromes, as well as between the algic and the trigger stages of muscle involvement. Such a differentiation does not involve biopsy in many cases.
Subject(s)
Creatine Kinase/blood , L-Lactate Dehydrogenase/blood , Lumbar Vertebrae , Muscular Dystrophies/blood , Nerve Compression Syndromes/blood , Osteochondritis/blood , Adult , Humans , Middle Aged , Muscular Dystrophies/etiology , Nerve Compression Syndromes/etiology , Osteochondritis/complicationsABSTRACT
The state of skeletal muscles was subjected to a comprehensive study in 106 patients with verified rheumatoid arthritis. Kinesthetic, thermography, electromyography, tensometry and morphohistochemical methods were employed together with determination of serum activity of creatine phosphokinase. The muscular syndrome of the disease was characterized by the presence of foci of myofibrosis manifesting as local painful indurations of different size and consistency. It has been shown that one of the mechanisms of muscular injury in rheumatoid arthritis is the neural reflex one: under the influence of pathological impulses from the damaged joint there occur tonic and dystrophic alterations in the muscle, vasomotor dysfunctions and derangement of the neurotrophic control.
Subject(s)
Arthritis, Rheumatoid/complications , Muscular Diseases/diagnosis , Adolescent , Adult , Aged , Arthritis, Rheumatoid/physiopathology , Chronic Disease , Fibrosis/diagnosis , Fibrosis/etiology , Fibrosis/physiopathology , Histocytochemistry , Humans , Methods , Middle Aged , Muscles/enzymology , Muscles/physiopathology , Muscular Diseases/etiology , Muscular Diseases/physiopathology , Terminology as TopicSubject(s)
Peripheral Nervous System Diseases/physiopathology , Adult , Animals , Humans , Male , Middle Aged , RatsSubject(s)
Arthritis, Rheumatoid/diagnosis , Muscular Diseases/diagnosis , Adult , Humans , Middle Aged , SyndromeSubject(s)
Arthritis, Rheumatoid/enzymology , Creatine Kinase/blood , Adult , Enzyme Activation , Female , Humans , Male , Middle AgedABSTRACT
The authors performed pathomorphological examinations in 19 patients with compressional-neural and myodystrophic syndromes of lumbar osteochondritis and also in 16 experimental animals with an impaired axoplasmic current. In the light of the modern understanding of the neurodystrophic control the authors discuss the mechanisms of the denervational-reinnervational process in muscles of patients with vertebrogenic pathology and myodystrophic disorders.
