ABSTRACT
A highly specific and readily reproducible cytochemical method for detecting DNA in nuclear structures of fixed cells has been developed. The method involves the reduction of metallic silver by aldehydes of the 2-deoxyribose of apurinic DNA from a solution of silver ammine prepared with allowance made for the requirements of the chemistry of the reaction. It was found that the method developed can be used for a quantitative analysis of DNA in animal cells.
Subject(s)
Apurinic Acid/analysis , Cell Nucleus/analysis , DNA/analysis , Polynucleotides/analysis , Aldehydes/analysis , Animals , Cattle , Chickens , Guinea Pigs , Liver/cytology , Male , Rana ridibunda , Sertoli Cells/cytology , Silver , Spermatozoa/cytology , Staining and LabelingABSTRACT
It was shown that serious errors in DNA cytofluorimetry may be due to incorrect illumination of photocathode of the photomultiplier in the cytofluorimeters and non-observance of the requirements to the nuclear structures of the cells under measurement with respect to their chemical integrity and thickness. The methods of the optimal illumination of photocathode in the cytofluorimeters and the criteria of selection of the nuclear structures on slides are considered with the aim of obtaining the objective results of DNA cytofluorimetry.