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1.

Use of the reduction of silver from silver ammine solution by the aldehyde groups of apurine DNA for the study of the chemistry of nuclear staining by the Feulgen method.

Khachaturov, E N.

Biol Bull Acad Sci USSR ; 7(5): 352-6, 1980.

Article in English | MEDLINE | ID: mdl-6172159

Subject(s)

Apurinic Acid/analysis , Cell Nucleus/analysis , DNA/analysis , Polynucleotides/analysis , Rosaniline Dyes , Aldehydes , Animals , Chickens , Coloring Agents , Erythrocytes/cytology , Silver , Staining and Labeling

2.

Cytochemical method of detecting DNA based on reduction of metallic silver from solution of silver ammine by aldehyde groups of apurinic DNA.

Khachaturov, E N.

Biol Bull Acad Sci USSR ; 7(3): 188-94, 1980.

Article in English | MEDLINE | ID: mdl-6172158

ABSTRACT

A highly specific and readily reproducible cytochemical method for detecting DNA in nuclear structures of fixed cells has been developed. The method involves the reduction of metallic silver by aldehydes of the 2-deoxyribose of apurinic DNA from a solution of silver ammine prepared with allowance made for the requirements of the chemistry of the reaction. It was found that the method developed can be used for a quantitative analysis of DNA in animal cells.

Subject(s)

Apurinic Acid/analysis , Cell Nucleus/analysis , DNA/analysis , Polynucleotides/analysis , Aldehydes/analysis , Animals , Cattle , Chickens , Guinea Pigs , Liver/cytology , Male , Rana ridibunda , Sertoli Cells/cytology , Silver , Spermatozoa/cytology , Staining and Labeling

3.

[Sources of several errors in DNA cytofluorimetry]. / Istochniki nekotorykh oshibok pri tsitofluorimetrii DNK.

Khachaturov, E N.

Ontogenez ; 8(2): 205-8, 1977.

Article in Russian | MEDLINE | ID: mdl-904854

ABSTRACT

It was shown that serious errors in DNA cytofluorimetry may be due to incorrect illumination of photocathode of the photomultiplier in the cytofluorimeters and non-observance of the requirements to the nuclear structures of the cells under measurement with respect to their chemical integrity and thickness. The methods of the optimal illumination of photocathode in the cytofluorimeters and the criteria of selection of the nuclear structures on slides are considered with the aim of obtaining the objective results of DNA cytofluorimetry.

Subject(s)

DNA/metabolism , Histocytochemistry/methods , Spectrometry, Fluorescence/methods

4.

[Cytochemical study of the substrate responsible for the differential staining of human chromosomes by length]. / Tsitokhimicheskoe izuchenie substrata, otvetstvennogo za differentsial'nuiu okrashivaemost' khromosom cheloveka po dline

Khachaturov, E N; Barskii, V E; Galkina, I G; Stonova, N S; Maksimal'do, Iu B.

Izv Akad Nauk SSSR Biol ; (6): 873-80, 1975.

Article in Russian | MEDLINE | ID: mdl-54381

Subject(s)

Chromosomes/metabolism , Coloring Agents/metabolism , Staining and Labeling , Animals , Cell Division , Female , Glycosaminoglycans/metabolism , Guinea Pigs , Humans , Lymphocytes/ultrastructure , Male , Mitosis , Spermatozoa/ultrastructure

5.

[Method of preventing the fading of luminescence of cell structure during the cytofluorimetric study of nucleic acids]. / Metod predtvrashcheniia vytsvetaniia liuminestsentsii kletochnykh struktur pri tsitofluorimetricheskom issledovanii nukleinovykh kislot

Khachaturov, E N.

Izv Akad Nauk SSSR Biol ; (2): 300-4, 1974.

Article in Russian | MEDLINE | ID: mdl-4618275

Subject(s)

DNA/analysis , RNA/analysis , Cytological Techniques , Fluorometry/methods

6.

[Application of microphotometry in reflected light for quantitative estimation of radioautographs]. / Primenenie mikrofotometrii v otrazhennom svete dlia kolichestvennoi otsenki radioavtografov.

Barskii, V E; Terskikh, V V; Khachaturov, E N.

Tsitologiia ; 13(1): 118-22, 1971 Jan.

Article in Russian | MEDLINE | ID: mdl-5090570

Subject(s)

Autoradiography , Photomicrography , Methods , Photomicrography/instrumentation

7.

[Comparative cytochemical study of mitochondria and kinetoplasts in representatives of the Trypanosomidae family]. / Sravnitel'noe tsitokhimicheskoe issledovanie mitokhondrii i kinetoplastov predstavitelei sem. Trypanosomidae.

Khachaturov, E N; Sukhareva-Nemakova, N N.

Tsitologiia ; 12(1): 77-84, 1970 Jan.

Article in Russian | MEDLINE | ID: mdl-4913049

Subject(s)

Mitochondria/analysis , Organoids/analysis , Succinate Dehydrogenase/analysis , Trypanosoma/cytology , Trypanosoma/enzymology , DNA/analysis , Histocytochemistry , Microscopy, Fluorescence , Microscopy, Phase-Contrast , Mitochondria/enzymology , Trypanosoma/analysis

8.

[Fluorescent-cytochemical study of DNA-containing organoids of Strigomonas oncopelti]. / Liuminestsentno-tsitokhimicheskoe issledovanie DNK-soderzhashchikh organoidov Strigomonas oncopelti.

Sukhareva-Nemakova, N N; Khachaturov, E N.

Tsitologiia ; 11(9): 1105-11, 1969 Sep.

Article in Russian | MEDLINE | ID: mdl-5380059

Subject(s)

DNA/analysis , Organoids/analysis , Trypanosoma , Acridines/pharmacology , Animals , Cell Division , Cell Nucleus/analysis , Culture Techniques , Histocytochemistry , Histones/analysis , Methods , Microscopy, Fluorescence , RNA/analysis , Trypanosoma/cytology , Trypanosoma/drug effects

9.

[Stoichiometric reaction of rivanol-SO2 with RNA, treated with anhydrous acetic acid]. / Stekhiometrichnost' reaktsii rivanola-SO2 s RNK, obrabotannoi bezvodnoi uksusnoi kislotoi.

Sherudilo, A I; Khachaturov, E N.

Izv Akad Nauk SSSR Biol ; 1: 154-6, 1969.

Article in Russian | MEDLINE | ID: mdl-5357511

Subject(s)

Acridines/pharmacology , Anti-Infective Agents/pharmacology , Lactates/pharmacology , RNA , Acetates/pharmacology , Chemical Phenomena , Chemistry , Sulfur Dioxide

10.

[Fluorescent methods of selective detection of RNA in different types of cells by means of the Schiff reaction]. / Liuminestsentnye metody izbiratel'nogo vyiavleniia RNK v keltkakh razlichnykh tipov s pomoshch'iu reaktsii Shiffa.

Khachaturov, E N.

Izv Akad Nauk SSSR Biol ; 3: 353-62, 1968.

Article in Russian | MEDLINE | ID: mdl-4903618

Subject(s)

Fluorescence , RNA/analysis , DNA/analysis , Histocytochemistry , Histological Techniques

11.

[Application of rivanol--SO2 for DNA cytofluorimetry]. / Primenenie rivanola-SO2 dlia tsitofluorimetrii DNK.

Khachaturov, E N; Smirnova, E A.

Izv Akad Nauk SSSR Biol ; 6: 900-5, 1966.

Article in Russian | MEDLINE | ID: mdl-4187869

Subject(s)

Acridines , DNA/analysis , Fluorometry , Staining and Labeling

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