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Klin Lab Diagn ; 61(2): 90-2, 102, 2016 Feb.
Article in Russian | MEDLINE | ID: mdl-27455562

ABSTRACT

The technique of immune blotting was used to analyze surface proteins obtained from cells M Tuberculosis exposed to partialmode of delipidization. At that, there were applied serums of patients with tuberculosis of lungs; HIV agents and patients with concomitant infection tuberculosis-AIDS and also HIV-negative patients without clinical signs of disease of lungs and with chronic diseases of lungs of other etiology The fractions oflow-molecular antigens with molecular mass 6.5-30 kilodaltons became diagnostically significant. To this fraction of antigens reacted serums of all patients with tuberculosis of lungs and serums of 91% of patients with concomitant tuberculosis-AIDS infection. The antigens of protein fractions with high (70-100 kilodaltons) and interim (30-69 kilodaltons) molecular mass became diagnostically insignificant.


Subject(s)
Antigen-Antibody Complex/blood , Antigens, Bacterial/isolation & purification , Immune Sera/chemistry , Mycobacterium tuberculosis/chemistry , Tuberculosis, Pulmonary/diagnosis , Adult , Antigens, Bacterial/immunology , Asthma/blood , Asthma/diagnosis , Asthma/immunology , Case-Control Studies , Coinfection , Diagnosis, Differential , Electrophoresis, Polyacrylamide Gel , Female , HIV Infections/blood , HIV Infections/diagnosis , HIV Infections/immunology , HIV Infections/virology , HIV-1/physiology , Humans , Male , Mycobacterium tuberculosis/immunology , Pneumonia, Bacterial/blood , Pneumonia, Bacterial/diagnosis , Pneumonia, Bacterial/immunology , Pulmonary Disease, Chronic Obstructive/blood , Pulmonary Disease, Chronic Obstructive/diagnosis , Pulmonary Disease, Chronic Obstructive/immunology , Tuberculosis, Pulmonary/blood , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/microbiology
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