Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging.

To understand the dynamics of tissue stiffness during neural tube formation and closure in a murine model, we have developed a multimodal, coaligned imaging system combining optical coherence tomography (OCT) and Brillouin microscopy. Brillouin microscopy can map the longitudinal modulus of tissue but cannot provide structural images. Thus, it is limited for imaging dynamic processes such as neural tube formation and closure. To overcome this limitation, we have combined Brillouin microscopy and OCT in one coaligned instrument. OCT provided depth-resolved structural imaging with a micrometer-scale spatial resolution to guide stiffness mapping by Brillouin modality. 2D structural and Brillouin frequency shift maps were acquired of mouse embryos at gestational day (GD) 8.5, 9.5, and 10.5 with the multimodal system. The results demonstrate the capability of the system to obtain structural and stiffness information simultaneously.

Multimodal high-resolution embryonic imaging with light sheet fluorescence microscopy and optical coherence tomography.

A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) was developed. LSFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously.

Multitwist Möbius Strips and Twisted Ribbons in the Polarization of Paraxial Light Beams.

The polarization of light can exhibit unusual features when singular optical beams are involved. In 3-dimensional polarized random media the polarization orientation around singularities describe 1/2 or 3/2 Möbius strips. It has been predicted that if singular beams intersect non-collinearly in free space, the polarization ellipse rotates forming many-turn Möbius strips or twisted ribbons along closed loops around a central singularity. These polarization features are important because polarization is an aspect of light that mediate strong interactions with matter, with potential for new applications. We examined the non-collinear superposition of two unfocused paraxial light beams when one of them carried an optical vortex and the other one a uniform phase front, both in orthogonal states of circular polarization. It is known that these superpositions in 2-dimensions produce space-variant patterns of polarization. Relying on the symmetry of the problem, we extracted the 3-dimensional patterns from projective measurements, and confirmed the formation of many-turn Möbius strips or twisted ribbons when the topological charge of one of the component beams was odd or even, respectively. The measurements agree well with the modelings and confirmed that these types of patterns occur at macroscopic length scales and in ordinary superposition situations.

Monstar polarization singularities with elliptically-symmetric q-plates.

Space-variant polarization patterns present in the transverse mode of optical beams highlight disclination patterns of polarization about a singularity, often a C-point. These patterns are important for understanding rotational dislocations and for characterizing complex polarization patterns. Liquid-crystal devices known as q-plates have been used to produce two of the three types of disclination patterns in optical beams: lemons and stars. Here we report the production of the third type of disclination, which is asymmetric, known as the monstar. We do so with elliptically-symmetric q-plates. We present theory and measurements, and find excellent agreement between the two.

Monstar disclinations in the polarization of singular optical beams.

The control of spatial and polarization modes of optical beams enables the production of topological singularities encoded on the polarization of the light. This allows the study of topological disclinations not easily found in the natural setting. In this article we report on the observation of new features in disclinations realized with singular optical beams. They were prepared using three spatial modes bearing optical vortices in non-separable superpositions with circular polarization states. The disclinations involve asymmetric rotational dislocations, whose optical counterparts in the optical far field are known as C-points, and which are classified as monstars. They have been known to have a singularity index that can be positive, or negative as reported by us recently. Here we report on monstars with an index of zero. Monstars are characterized by having sectors bound by radial lines that involve curved lines radiating from the singularity. We found that kinks in otherwise smooth line patterns of asymmetric disclinations are scars of a separate but related pattern of line-slope discontinuities, carried optically by C-lines in the far field. These scars are indicative of the underlying structure or symmetry of the pattern. We present a general formalism to understand and generate monstars, along with measurements: the experimental results are in excellent agreement with theoretical modelings.

Determining topological charge of an optical beam using a wedged optical flat.

The topological charge of a beam carrying an optical vortex is an important parameter that specifies the amount of orbital angular momentum carried by the beam and the azimuthal order of the beam mode. We present an experimental method to determine the sign and magnitude of the topological charge using a wedged optical flat as a lateral shearing interferometer. When the curvature of the wavefront is adjusted to be planar, the fringe pattern generated by the shearing interferometer consists of two conjoined forks that unambiguously identify the topological charge of the beam. We also investigated the changes in the pattern when the wedged flat is rotated.