ABSTRACT
BACKGROUND: The present study investigates the driving effects of globalization on the urban environment in two countries of Italy and Japan, which have the regular amplified economy among the advanced countries. For this purpose, a model with the collaboration of two main subjects of globalization coverage and urbanization and the methodological procedures of correlation test and structural analysis was constructed. A globalization index, namely the Maastricht globalization index (MGI), was assumed based on the integrated values of ten factors [HDI, ITA, GDP, FDI, TEI, GEE, GME, MCS, and IUI] besides three ecological indicators as the baseline of the urban environment, namely carbon dioxide emission (CDE), municipal solid wastes (MSW), and wastewater treatment plants (WTP). RESULTS: Results revealed the positive associations between globalization and wastewater treatment of urban areas in both countries, exposing the influential role of globalization in connecting the urban population to the sewage plants. The results confirmed the positive role of globalization in decreasing carbon dioxide emissions and overall its practical influences to mitigate urban air pollution. However, the overall globalization effect on urban waste production was estimated differently in both countries. CONCLUSIONS: Based on the MICMAC analysis, only three factors, namely HDI, ITA, GDP, and FDI, can express driving powers and a significant share of globalization coverage. Consequently, enhancing such indicators that belong to globalization's social and economic domains certainly can act as driver powers to mitigate the environmental issues of urbanization in the study areas.
ABSTRACT
Fowlpox (FP) is a viral disease that is widely distributed throughout the world. The disease has an economic impact on the poultry industry, and its prevalence has even been reported in vaccinated flocks. The present study used flow cytometry to evaluate the CD4+ and CD8+ T-cell immune response of chicks induced by FP vaccine. 120 specific pathogen-free (SPF) 21-day-old chicks were randomly divided into three groups of 40. One group was used as negative control with PBS inoculation, the other two groups were inoculated with the local fowlpox vaccine produced by Razi Institute and commercial FP vaccines, and they were kept for five weeks. Peripheral blood mononuclear cells (PBMC) were isolated using Ficoll-Hypaque density gradients and the percentages of CD3+, CD3+, CD4+, and CD3+CD8+ T lymphocytes were analyzed with flow cytometry. Seven days post-immunization, a maximum (90-100%) swelling formation ("take") on the vaccination site was observed. The ratios of CD4+ to CD8+ T-lymphocytes in both vaccinated groups were significantly higher (p < 0.05) than the control group inoculated with PBS. The percentages of CD3+, CD3+CD4+, and CD3+CD8+ T-lymphocytes were increased in chickens vaccinated with commercial and local FP vaccines. There were no significant differences between the groups receiving commercial and local fowl pox vaccines. The present study showed that protective immunity could be associated with increased cellular immune responses, which has been interpreted as enhancing T-cell proliferation and increasing CD4+ to CD8+ ratios through vaccination with the FP vaccine. This study further suggests that the induction of enhanced immune responses is due mainly to the Th1-type response.
