Elevated CO2 Can Improve the Tolerance of Avena sativa to Cope with Zirconium Pollution by Enhancing ROS Homeostasis.

Zirconium (Zr) is one of the toxic metals that are heavily incorporated into the ecosystem due to intensive human activities. Their accumulation in the ecosystem disrupts the food chain, causing undesired alterations. Despite Zr's phytotoxicity, its impact on plant growth and redox status remains unclear, particularly if combined with elevated CO2 (eCO2). Therefore, a greenhouse pot experiment was conducted to test the hypothesis that eCO2 can alleviate the phytotoxic impact of Zr upon oat (Avena sativa) plants by enhancing their growth and redox homeostasis. A complete randomized block experimental design (CRBD) was applied to test our hypothesis. Generally, contamination with Zr strikingly diminished the biomass and photosynthetic efficiency of oat plants. Accordingly, contamination with Zr triggered remarkable oxidative damage in oat plants, with concomitant alteration in the antioxidant defense system of oat plants. Contrarily, elevated levels of CO2 (eCO2) significantly mitigated the adverse effect of Zr upon both fresh and dry weights as well as the photosynthesis of oat plants. The improved photosynthesis consequently quenched the oxidative damage caused by Zr by reducing the levels of both H2O2 and MDA. Moreover, eCO2 augmented the total antioxidant capacity with the concomitant accumulation of molecular antioxidants (e.g., polyphenols, flavonoids). In addition, eCO2 not only improved the activities of antioxidant enzymes such as peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) but also boosted the ASC/GSH metabolic pool that plays a pivotal role in regulating redox homeostasis in plant cells. In this regard, our research offers a novel perspective by delving into the previously unexplored realm of the alleviative effects of eCO2. It sheds light on how eCO2 distinctively mitigates oxidative stress induced by Zr, achieving this by orchestrating adjustments to the redox balance within oat plants.

Soil Contamination with Europium Induces Reduced Oxidative Damage in Hordeum vulgare Grown in a CO2-Enriched Environment.

The extensive and uncontrolled utilization of rare earth elements, like europium (Eu), could lead to their accumulation in soils and biota. Herein, we investigated the impact of Eu on the growth, photosynthesis, and redox homeostasis in barley and how that could be affected by the future CO2 climate (eCO2). The plants were exposed to 1.09 mmol Eu3+/kg soil under either ambient CO2 (420 ppm, aCO2) or eCO2 (620 ppm). The soil application of Eu induced its accumulation in the plant shoots and caused significant reductions in biomass- and photosynthesis-related parameters, i.e., chlorophyll content, photochemical efficiency of PSII, Rubisco activity, and photosynthesis rate. Further, Eu induced oxidative stress as indicated by higher levels of H2O2 and lipid peroxidation products, and lower ASC/DHA and GSH/GSSG ratios. Interestingly, the co-application of eCO2 significantly reduced the accumulation of Eu in plant tissues. Elevated CO2 reduced the Eu-induced oxidative damage by supporting the antioxidant defense mechanisms, i.e., ROS-scavenging molecules (carotenoids, flavonoids, and polyphenols), enzymes (CAT and peroxidases), and ASC-GSH recycling enzymes (MDHAR and GR). Further, eCO2 improved the metal detoxification capacity by upregulating GST activity. Overall, these results provide the first comprehensive report for Eu-induced oxidative phytotoxicity and how this could be mitigated by eCO2.

Plants from a semi-arid environment as a source of phytochemicals against Fusarium crown and foot rot in zucchini.

Fusarium crown and foot rot, caused by F. solani f. sp. cucurbitae, are major fungal diseases affecting zucchini and other cucurbits. Despite the efficacy of synthetic fungicides, their health and environmental hazards have highlighted the urgent need for safer alternatives, such as phytochemical-based biocides. Owing to the upregulation of the plant secondary metabolism under stressful conditions, bioprospecting in harsh environments could reveal ore plants for bioactive metabolites. In this study, thirteen wild plants were collected from their natural habitat in a semiarid environment (Yanbu, Saudi Arabia) and extracted to obtain phenolics rich extracts. Total polyphenols, flavonoids, antioxidant capacities and the antifungal activities of the extracts against a pathogenic isolate of F. solani were assessed. Fusarium solani was isolated from infected zucchini and characterized by scanning electron microscopy. Hierarchical clustering analysis of the phytochemical screening and in vitro bioactivity revealed that Rosmarinus officinalis, Pulicaria crispa, Achillea falcata and Haloxylon salicornicum were the richest in polyphenols and the most powerful against F. solani. Further, the extracts of these four plants significantly decreased the disease incidence in zucchini, where P. crispa was the premier. Interestingly, results of transmission electron microscopy revealed that extract of P. crispa, as a representative of the powerful group, induced ultrastructural disorders in fungal cells. Therefore, this study suggests the use of R. officinalis, P. crispa, A. falcata and H. salicornicum grown in semi-arid environments as ore plants to develop phytochemical-based biocides against Fusarium crown and foot rot.

Potential of biosynthesized zinc oxide nanoparticles to control Fusarium wilt disease in eggplant (Solanum melongena) and promote plant growth.

In this study, a novel, non-toxic, eco-friendly zinc oxide nanoparticles (ZnO-NPs) was used instead of the synthetic fungicides widely used to control the destructive phytopathogenic fungus Fusarium oxysporum, the causative agent of wilt disease in Solanum melongena L. Herein, the biosynthesized ZnO-NPs was carried out by Penicillium expansum ATCC 7861. In vitro, mycosynthesized ZnO-NPs exhibited antifungal activity against Fusarium oxysporum. In vivo, ZnO-NPs suppressed Fusarium wilt disease in cultivated Solanum melongena L. by decreasing the disease severity with 75% of plant protection. Moreover, ZnO-NPs stimulated the recovery of eggplant as an indicated by improving of morphological and metabolic indicators including plant height(152.5%), root length(106.6%), plant fresh biomass (146%), chlorophyll a (102.8%), chlorophyll b (67.86%), total soluble carbohydrates (48.5%), total soluble protein (81.8%), phenol (10.5%), antioxidant activity and isozymes compared with infected control. Therefore, this study suggests using mycosynthesized ZnO-NPs as an alternative to synthetic fungicides not only to eradicate the Fusarium wilt disease in cultivated eggplant (Solanum melongena) but also to promote the growth parameters and metabolic aspects.

Enhancement of Seawater Stress Tolerance in Barley by the Endophytic Fungus Aspergillus ochraceus.

Symbiotic plant-fungi interaction is a promising approach to alleviate salt stress in plants. Moreover, endophytic fungi are well known to promote the growth of various crop plants. Herein, seven fungal endophytes were screened for salt tolerance; the results revealed that Aspergillus ochraceus showed a great potentiality in terms of salt tolerance, up to 200 g L-1. The indole acetic acid (IAA) production antioxidant capacity and antifungal activity of A. ochraceus were evaluated, in vitro, under two levels of seawater stress, 15 and 30% (v/v; seawater/distilled water). The results illustrated that A. ochraceus could produce about 146 and 176 µg mL-1 IAA in 15 and 30% seawater, respectively. The yield of IAA by A. ochraceus at 30% seawater was significantly higher at all tryptophan concentrations, as compared with that at 15% seawater. Moreover, the antioxidant activity of ethyl acetate extract of A. ochraceus (1000 µg mL-1) at 15 and 30% seawater was 95.83 ± 1.25 and 98.33 ± 0.57%, respectively. Crude extracts of A. ochraceus obtained at 15 and 30% seawater exhibited significant antifungal activity against F. oxysporum, compared to distilled water. The irrigation of barley plants with seawater (15 and 30%) caused notable declines in most morphological indices, pigments, sugars, proteins, and yield characteristics, while increasing the contents of proline, malondialdehyde, and hydrogen peroxide and the activities of antioxidant enzymes. On the other hand, the application of A. ochraceus mitigated the harmful effects of seawater on the growth and physiology of barley plants. Therefore, this study suggests that the endophytic fungus A. ochraceus MT089958 could be applied as a strategy for mitigating the stress imposed by seawater irrigation in barley plants and, therefore, improving crop growth and productivity.

Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants.

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.

Xylariaceae on the fringe.

The Xylariaceae is one of the best-known pyrenomycete families (Ascomycota) and is distributed throughout the world. The majority are wood inhabitants and are prevalent in tropical and subtropical regions. Halorosellinia oceanicum is the most widely distributed in mangroves and can be regarded as truly manglicolous being frequently recorded as the dominant member of the family in such environments in S.E. Asia. In Malaysian mangroves, members of the Xylariaceae have been found to be numerically important with up to 9% present in one mangrove ecosystem. A further twelve xylariaceous genera are reported as occurring as their teleomorphs in mangrove forest and their immediate surroundings including Anthostomella, Astrocystis, Biscogniauxia, Camillea, Daldinia, Fasciatispora, Hypoxylon, Kretzschmaria, Nemania, Nipicola, Rosellinia and Xylaria. Furthermore, the presence of species from a number of these taxa, especially species of Anthostomella and Xylaria, are regularly isolated as endophytes from a variety of mangrove plant species. Mangrove Xylariaceae are also well known for their ability to produce novel and often bioactive metabolites.