Aspergillus flavus and Aspergillus ochraceus inhibition and reduction of aflatoxins and ochratoxin A in maize by irradiation.

Grains are susceptible to contamination by molds; some cause spoilage and others produce certain mycotoxins that cause a serious health threat to humans and animals. Aspergillus flavus and Aspergillus ochraceus and their mycotoxins, aflatoxins and ochratoxin A, are natural contaminants of various agricultural commodities. Control of these molds and their mycotoxins in food commodities is of utmost importance; therefore, the target of this research was to explore the effects of gamma irradiation doses on the growth of A. flavus and A. ochraceus in artificially inoculated yellow maize as well as on the production of aflatoxin B1, ochratoxin A, and the formation of toxins in maize. The irradiated dose of 6.0 kGy was found to completely inhibit the growth of the two molds, while a dose of 4.5 kGy reduced the production of their mycotoxins. Maximum degradation of the formed aflatoxins and ochratoxin A in maize occurred at 20 kGy, with best reduction rates of 40.1%, 33.3%, and 61.1% observed for aflatoxin B1, aflatoxin B2, and ochratoxin A, respectively. We recommend grains irradiation by gamma radiation at 6.0 kGy to decontaminate mycotoxin-producing molds before they produce mycotoxins. The study represents a proactive, efficient, and potent method for avoiding potential contamination of fungus during grains storage and transfer for one to two months.

Biodegradation of 3-chlorobenzoic acid with electron shuttle systems: pathways and molecular identification.

A synergy of biodegradation and electron shuttle systems is a promising strategy for eliminating pollutants including chlorinated aromatic compounds. The present work studies the degradation products of 3-chlorobenzoic acid by Pseudomonas putida in the presence of an electron shuttle system (ESS) composed of citrate and pyruvate as electron donors and the pollutant as an electron acceptor. Chromatographic results showed different pathways involved in the biodegradation process under the influence of electron shuttle systems. These routes depend on oxidation and reduction reactions for output byproducts to be easily mineralized by the bacterium under investigation. A nucleotide sequence with about 380 bp of a ton B gene was detected in P. putida and it resembles Escherichia coli Ton B. The relatedness tree of the selected gene reveals a high similarity and is comparable to P. aeruginosa (100%) and the highest variation with that of P. citronellolis (21.99%). Accordingly, in the presence of electron shuttle systems, the genes responsible for bacterial influx were activated to ease the biodegradation process. In an application model, the remediated-water samples were handled by two recycling processes using Scenedesmus obliquus and Trigonella foenum-graecum to evaluate the efficiency of this non-conventional treatment. In conclusion, this strategy succeeded in remediating the polluted water with chlorinated aromatic compounds for further applications.

Bacterial-mediated biodegradation of pentachlorophenol via electron shuttling.

Pentachlorophenol (PCP) degradation by soil indigenous bacteria represents a practical and cost-effective solution. In the present study, bacteria isolated from paddy soil was investigated and the role of electron shuttling (ES) in the PCP degradation process was assessed. Two strains demonstrated the highest PCP degradation of 93.5% and 94.88% in the presence of citrate and were identified using 16S rRNA phylogenetic analysis as Pseudomonas chengduensis and Pseudomonas plecoglossicida, respectively. Both strains showed higher PCP degradation in free form as opposed to a reduced activity in immobilized and respiratory impaired form. The addition of pyruvate resulted in about 80% PCP degradation in 5 days for P. chengduensis, on the other hand, P. plecoglossicida showed the same result under anaerobic conditions whether pyruvate was added or not. Phenazine and the outer membrane c-type cytochrome were reported only for P. chengduensis as opposed to P. plecoglossicida. The results indicate that despite following different approaches in PCP degradation, both strains are useful in PCP clean-up under aerobic and anaerobic conditions and in free direct contact. The degradation is enhanced via ES. This is considered both an effective and feasible technology for in situ clean-up of contaminated sites or on-site bioreactors.