Evaluation of automated monitoring calving prediction in dairy buffaloes a new tool for calving management.

Buffalo is one of the leading milk-producing dairy animals. Its production and reproduction are affected due to some factors including inadequate monitoring around parturition, which cause economic losses like delayed birth process, increased risk of stillbirth, etc. The appropriate calving monitoring is essential for dairy herd management. Therefore, we designed a study its aim was, to predict the calving based on automated machine measured prepartum behaviors in buffaloes. The data were collected from n=40 pregnant buffaloes of 2nd to 5th parity, which was synchronized. The NEDAP neck and leg logger tag was attached to each buffalo at 30 days before calving and automatically collected feeding, rumination, lying, standing, no. of steps, no. of switches from standing to lying (lying bouts) and total motion activity. All behavioral data were reduced to -10 days before the calving date for statistical analysis to use mixed model procedure and ANOVA. Results showed that feeding and rumination time significantly (P<0.05) decreased from -10 to -1 days before calving indicating calving prediction. Moreover, Rumination time was at lowest (P<0.001) value at 2h before the calving such behavioral changes may be useful to predict calving in buffaloes. Similarly, lying bouts and standing time abruptly decreased (P<0.05) from -3 to -1 days before calving, while lying time abruptly increased (P<0.01) from -3 to -1 days before calving (531.57±23.65 to 665.62±18.14, respectively). No. of steps taken and total motion significantly (P<0.05) increased from -10 to -1 days before calving. Feeding time was significantly (P<0.02) lowered in 3rd parity buffaloes compared with 2nd, 4th and 5th parity buffaloes, while standing time of 5th parity buffaloes were lowered (P<0.05) as compared to 2nd to 4th parity buffalos at -1 day of prepartum. However, rumination, lying, no. of steps taken and total motion activity at -1 day of prepartum was independent (P>0.05) of parity in buffaloes. Neural network analysis for combined variables from NEDAP technology at the daily level yielded 100.0% sensitivity and 98% specificity. In conclusion NEDAP technology can be used to measured behavioral changes -10 day before calving as it can serve as a useful guide in the prediction calving date in the buffaloes.

Exploring nutritive profile, metabolizable energy, protein, and digestible amino acids contents of indigenous protein sources of different locations for male broilers.

2 experiments were conducted to explore nutrient composition, AME, AMEn, standardized ileal digestibility (SID) of CP, and amino acids (AA) of 4 indigenous protein sources including canola meal (CM), rapeseed meal (RSM), guar meal (GM), and sunflower meal (SFM) collected from 2 different locations, Multan (MUL; n = 3) and Sukkur (SKR; n = 3), of Pakistan. Higher (P < 0.05) dry matter (DM), CP, and gross energy (GE), whereas lower (P < 0.05) ash contents were found in SKR, CM, and RSM compared with those from MUL. The MUL GM had higher (P < 0.05) crude fiber (CF) and CP, whereas lower (P < 0.05) GE compared with those from SKR. The SFM from MUL had higher DM, whereas lower CF and CP contents than SKR. In the first experiment, 216 21-d-old male broilers (Ross 308) were distributed over 8 test diets (4 ingredients × 2 locations) and 1 basal diet, with 4 replicates containing 6 birds each (9 × 4 × 6), in a complete randomized design to determine AME and AMEn. The results indicated higher (P < 0.05) AME and AMEn in MUL CM than SKR. In the second experiment, 216 21-d-old male broilers (Ross 308) were raised in 36 cages (6 birds each) to determine SID of CP and AA in a complete randomized design. 8 test diets (4 ingredients × 2 locations) and a protein-free diet, with 4 replicates each, were tested. The SID of CP and some AA were higher (P < 0.05) in MUL CM and RSM than SKR. The SKR GM had a higher (P < 0.05) SID of CP, arginine, methionine, threonine, valine, and cysteine compared with that from MUL. The SFM from MUL had higher (P < 0.05) SID of CP, arginine, histidine, methionine, valine, alanine, aspartate, cysteine, and serine than SKR. In conclusion, major differences do exist between CM, GM, RSM, and SFM from different locations in terms of nutrients, AME, digestible CP, and AA contents for male broilers.

Nutritional and therapeutic perspectives of Chia (Salvia hispanica L.): a review.

The ancient grain is becoming enormously popular in modern food regimen in many countries; the higher proportion of α-linolenic acid makes chia the superb source of omega-3 fatty (about 65 % of the oil content). Omega-3 fatty acid has been associated with a large number of physiological functions in human body. Chia seed is a potential source of antioxidants with the presence of chlorogenic acid, caffeic acid, myricetin, quercetin, and kaempferol which are believed to have cardiac, hepatic protective effects, anti-ageing and anti-carcinogenic characteristics. It is also a great source of dietary fibre which is beneficial for the digestive system and controlling diabetes mellitus with higher concentration of beneficial unsaturated fatty acids, gluten free protein, vitamin, minerals and phenolic compounds. Therapeutic effects of chia in the control of diabetes, dyslipidaemia, hypertension, as anti-inflammatory, antioxidant, anti-blood clotting, laxative, antidepressant, antianxiety, analgesic, vision and immune improver is scientifically established.

Distribution, correlation, and source apportionment of selected metals in tannery effluents, related soils, and groundwater--a case study from Multan, Pakistan.

In order to study the distribution, correlation, and apportionment of selected metals, the tannery effluent, related soil, and groundwater samples were collected from Multan, Pakistan, and analyzed on flame atomic absorption spectrophotometer. Among the selected metals, Na, Ca, K, Cr, and Mg revealed dominant concentrations with average values of 5,499, 945, 565, 209, and 107 mg/L and 2,634, 330, 484, 14.1, and 60.5 mg/L in the effluents and groundwater, respectively, whereas the mean metal levels in soil samples were 10,026, 6,726, 9,242, 476, and 9,857 mg/kg. Overall, the mean metal concentrations in the tannery effluents, groundwater, and related soils reveal following order, respectively: Na > Ca > K > Cr > Mg > Ni > Fe > Zn > Co > Pb > Mn > Cd; Na > K > Ca > Mg > Cr > Zn > Ni > Pb > Fe > Co > Mn > Cd; Na > Mg > K > Ca > Cr > Co > Ni > Fe > Pb > Mn > Zn > Cd. Generally, the metal distribution in tannery effluents, soils, and groundwater was found to be random as evidenced by large differences between mean and median values as well as considerably higher standard deviation and skewness values. The selected metal data were also subjected to correlation study to investigate the covariation of metal levels in the three media. The source apportionment of the metal data in the effluents, soils, and groundwater was carried out using principal component analysis in addition to basic statistical and correlation analyses. The source apportionment studies evidenced the gross contamination of groundwater and soils in the vicinity of tanning industrial units in Multan. The current mean metal levels in the soil and groundwater were found to be considerably higher compared with the background concentration levels and WHO guideline values.

Statistical source identification of metals in groundwater exposed to industrial contamination.

Levels of selected metals Na, Ca, Mg, K, Fe, Mn, Cr, Co, Ni, Cd, Pb and Mn were estimated by flame atomic absorption spectrophotometry in groundwater samples from Kasur, a significant industrial city of Pakistan. Salient mean concentration levels were recorded for: Na (211 mg/l), Ca (187 mg/l), Mg (122 mg/l), K (87.7 mg/l), Fe (2.57 mg/l) and Cr (2.12 mg/l). Overall, the decreasing metal concentration order was: Na>Ca>Mg>K>Fe>Cr>Zn>Co>Pb>Mn>Ni>Cd. Significantly positive correlations were found between Na-Cr (r=0.553), Na-Mn (r=0.543), Mg-Fe (r=0.519), Mg-Cr (r=0.535), Pb-K (r=0.506) and Pb-Ni (r=0.611). Principal Component Analysis and Cluster Analysis identified tannery effluents as the main source of metal contamination of the groundwater. The present metal data showed that Cr, Pb and Fe levels were several times higher than those recommended for water quality by WHO, US-EPA, EU and Japan. The elevated levels of Cr, recorded as 21-42 fold higher compared with the recommended quality values, were believed to originate from the tanning industry of Kasur.

Multivariate analysis of the selected metals in the hair of cerebral palsy patients versus controls.

Seventeen metals were measured in scalp hair samples from cerebral palsy patients (CPPs) and controls. Samples were collected from 95 CPPs and 93 controls. The nitric acid-perchloric acid wet digestion procedure was used for quantification of the selected metals by flame atomic absorption spectrophotometry. The concentrations of Ag, Ca, Cd, Co, Cr, Li, and Mg were significantly higher and those of Cu, Fe, K, Mn, Na, Ni, Pb, and Sb were lower in the hair of CPPs compared with controls. Astrong positive correlation was found between Ca and Mg in the hair of controls but not in that of CPPs. Antimony was found significantly negative in terms of its correlation with Co and Cu in CPPs group but not in the controls. Principal component analysis (PCA) of the data extracted seven factors for CPPs and six factors for controls. Cluster analysis (CA) was also used to support the PCA results. The study evidenced some specific source of Mg and Sb in the hair of CPPs.

Multivariate analysis of trace metals in textile effluents in relation to soil and groundwater.

This paper deals with the multivariate analysis of metal data in effluents, soil and groundwater to find the distribution and source identification of the selected metals in the three media. Samples were collected from three textile industries located in Hattar Industrial Estate, Pakistan. Metals were estimated by flame atomic absorption spectrophotometry. The results showed elevated levels of Cr, Pb, Ni, Co, Fe, Ca, Na, K and Zn in these media, following the order: soil>effluent>water. Principle component analysis (PCA) of the data showed that the textile effluents are contaminating the soil wherein Cr and Pb were dominant toxic metals having concentrations of 5.96 mg/kg and 4.46 mg/kg, respectively. Other toxic metals such as Co, Cd, Zn, Ni, Mn and Fe, were found to have common origin in the textile effluents. The correlation study along with linear regression and PCA, supported the fact that various elevated metal concentrations emerged from the textile industrial effluents ultimately leading to contamination of the soil and groundwater in their proximity. The estimated metal levels in the water/soil system are compared with the safe limits laid down by WHO.

Comparative metal distribution in hair of Pakistani and Libyan population and source identification by multivariate analysis.

Using nitric acid-perchloric acid wet digestion based FAAS method ten selected metals (Cd, Co, Cr, Fe, K, Mn, Na, Ni, Pb and Zn) were determined in the scalp hair of male Pakistani and Libyan donors (n = 62), between 3-54 years age and residing in typical urban areas. The study was taken up to identify sources of metal distribution in two diverse population segments exposed to different environments. Sodium emerged with the highest mean hair concentration of 511.0 microg/g and 496.9 mug/g for Libyan and Pakistani donors respectively, followed by K, at 266.2 microg/g and 242.4 microg/g for the two donor categories. Cadmium showed the lowest mean hair concentration for both Pakistani (0.380 microg/g) and Libyan (0.530 microg/g) donors. Levels of Na, K, Pb, Cr and Cd were higher in hair of Libyan donors compared with Pakistani counterparts which showed higher comparative levels of Zn, Fe, Co, Ni and Mn. Strong metal-to-metal correlations were found between Na-K (r = 0.638) and Cd-Co (r = 0.574) for Pakistani donors, while for Libyan donors, Na-K (r = 0.680) and Fe-Mn (r = 0.624) correlations emerged as strongly significant. Only K was found to have a strong positive correlation (r = 0.611) with age for Pakistani donors while this correlation was significantly negative (r = -0.500) for Libyan donors, probably arising from individual food habits. Other metals showed no viable relationship with age. Principal Component Analysis (PCA) and Cluster Analysis (CA) of the data evidenced food, industrial emissions and automobile emissions as possible sources of metal distribution. The results of the present study are compared with those reported for subjects from other regions of the world.

Spatial variations in selected metal contents and particle size distribution in an urban and rural atmosphere of Islamabad, Pakistan.

Spatial variations in total suspended particulate matter (TSP) were investigated for distribution of metals and particle size fractions in the urban and rural atmosphere of Islamabad, Pakistan. The metals Na, K, Fe, Mn, Pb, Cd, Cr, Zn, Ni and Co, and the particle fractions <2.5, 2.5-10, 10-100 and >100 microm were included in the study. TSP samples were trapped on glass fibre filters using high volume samplers and quantification of metals was done using Atomic Absorption Spectrometry employing HNO(3) based wet digestion. At the urban site, Na was dominant at 2.384 microg/m(3) followed by K, Fe and Zn with 0.778, 0.667 and 0.567 microg/m(3) as mean concentrations, respectively. The metal levels for the rural site ranged from 0.002 microg/m(3) for Cd to 1.077 microg/m(3) for Na. However, compared with the urban site, mean Pb concentration showed an almost two-fold enhancement, i.e., 0.163 Vs. 0.327 microg/m(3). Metals and particle size source identification was done using Principal Component Analysis and Cluster Analysis. Five sources were traced out for the urban site: industrial, soil, automobile emissions, metallurgical industries and excavation activities. For the rural site, four sources were recorded: agricultural, automotive emissions, excavation activities and metallurgical units. Collectively, for both the sites, PM(10-100) emerged as a major contributor to TSP, followed by PM(2.5-10), PM(<2.5) and PM(>100) in that order. The metals showed in general positive relationship with fine particulate fractions (PM(2.5-10), PM(<2.5)), and negative correlation with coarse fractions (PM(10-100), PM(>100)). Comparison with the corresponding data from various Asian sites revealed that the levels of Na, K, Fe, Mn, Co and Ni for the present study were lower than those reported for grossly polluted cities of the world.

Multivariate analysis of trace metal levels in tannery effluents in relation to soil and water: a case study from Peshawar, Pakistan.

Tannery effluents and relevant ground water and soil samples collected from various tanning industries of Peshawar were analyzed for Na, Ca, K, Mg, Fe, Mn, Cr, Co, Cd, Ni, Pb and Zn by the AAS method. The metal concentration data for the three media are reported in terms of basic statistical parameters, metal-to-metal correlations and linear regression analyses. Metal distributions in the three media were quite divergent and showed non-normal distributions with high standard deviation and skewness parameters. Sodium exhibited the highest mean levels of 1,277mg/L, 881mg/L and 12,912mg/kg in the effluent, ground water and soil samples, respectively. Among other metals, Cr concentrations were 410mg/L, 0.145mg/L, 100mg/kg and Ca, 278mg/L, 64.8mg/L, and 2,285mg/kg in the effluent, ground water and soil samples, respectively. Some significant correlations were observed between effluent and soils in terms of Na, Cr, Ni, Co and Pb. The ground water-soil interrelationship suggested that Na levels in the soil and ground water were significantly correlated with each other (r=0.486, P<0.01). Similarly, Cr in the soil is strongly correlated with Ca in ground water (r=0.486, P<0.01). These results were duly supported by the linear regression analysis of data. The source identification studies conducted using Principal Component Analysis (PCA) and Cluster Analysis (CA) evidenced that ground water and soil were being contaminated by the toxic metals emanating from the tannery effluents.

Multivariate analysis of selected metals in tannery effluents and related soil.

Effluent and relevant soil samples from 38 tanning units housed in Kasur, Pakistan, were obtained for metal analysis by flame atomic absorption spectrophotometric method. The levels of 12 metals, Na, Ca, K, Mg, Fe, Mn, Cr, Co, Cd, Ni, Pb and Zn were determined in the two media. The data were evaluated towards metal distribution and metal-to-metal correlations. The study evidenced enhanced levels of Cr (391, 16.7 mg/L) and Na (25,519, 9369 mg/L) in tannery effluents and relevant soil samples, respectively. The effluent versus soil trace metal content relationship confirmed that the effluent Cr was strongly correlated with soil Cr. For metal source identification the techniques of principal component analysis, and cluster analysis were applied. The principal component analysis yielded two factors for effluents: factor 1 (49.6% variance) showed significant loading for Ca, Fe, Mn, Cr, Cd, Ni, Pb and Zn, referring to a tanning related source for these metals, and factor 2 (12.6% variance) with higher loadings of Na, K, Mg and Co, was associated with the processes during the skin/hide treatment. Similarly, two factors with a cumulative variance of 34.8% were obtained for soil samples: factor 1 manifested the contribution from Mg, Mn, Co, Cd, Ni and Pb, which though soil-based is basically effluent-derived, while factor 2 was found associated with Na, K, Ca, Cr and Zn which referred to a tannery-based source. The dendograms obtained from cluster analysis, also support the observed results. The study exhibits a gross pollution of soils with Cr at levels far exceeding the stipulated safe limit laid down for tannery effluents.

A comparative study based on gender and age dependence of selected metals in scalp hair.

Levels of 10 metals (Ca, Mg, Fe, Zn, Cu, Mn, Cd, Co, Cr and Ni) in the scalp hair of male and female donors, with age groups between 3 and 100 years, were determined by ICP-AES. In total, 58 male and 30 female hair samples were analyzed in triplicate. The donors belonged to the rural area of district Chakwal, a well-known typical non-urban site in Pakistan. Calcium showed the highest concentration of 462 microg/g in the hair of males and 870 microg/g in those of females followed by Zn, at 208 and 251 microg/g for the two sexes. For male donors, Cd showed the lowest concentration (1.15 microg/g) while for female donors Co remained at minimum level (0.92 microg/g). The order of decreasing metal concentration in the hair of male donors was: Ca > Zn > Mg > Fe > Cu > Mn > Ni > Cr > Co > Cd while that for female donors it was: Ca > Zn > Mg > Fe > Cu > Mn > Cr > Ni > Cd > Co. The female group exhibited enhanced levels of all selected metals except Fe and Co in their hair as compared with the male counterparts. A strong bivariate positive correlation was found between Fe and Zn (r = 0.841) for the hair samples from male category while for the female category, strong positive correlations were observed between Ca-Mg (r = 0.617), Ca-Zn (r = 0.569), Ca-Mn (r = 0.565), Mg-Mn (r = 0.655), Cr-Cu (r = 0.655) and Cr-Ni (r = 0.685). The distribution of metals in the hair of donors with respect to different age groups was also investigated for both genders. The study showed that in case of males, the concentration of all selected metals decreased with increasing age except for Cu, Co and Cr. However, for females the hair metal levels increased with age, except for Co for which the concentration decreased with age. No appreciable change in the metal concentration was observed as a function of age for the combined sexes.

Profile of malignancies at Medical College, Ambajogai--(15 years retrospective study).

Present study comprises of a preliminary analysis of malignant lesions, diagnosed and confirmed by histopathological examination, carried out in Department of Pathology, S. R. T. R. Medical College, Ambajogai, from August 1976 to July 1991. 30,168 specimens were received for histopathological examination out of which 2,663 cases were diagnosed as malignant lesions (8.82%). 163 cases of leukemias were diagnosed by peripheral smear and bone marrow examination. Our of 2826 patients with malignant lesions 1,566 (55.41%) were males and 1,260 (44.59%) were females with M:F ratio as 1.24:1. Analysis of all malignant lesions showed more predilection towards genital organs (26.22%), gastrointestinal tract (13.72%) and breast (10.47%). Penis (11.36%), gastrointestinal tract (16.73%), oral cavity and tongue with pharynx (20.81%) and skin (11.11%) were leading sites in males where as cervix (30.63%) breast (22.46%), gastrointestinal tract (10.00%), skin (6.82%) and lymphoma, leukaemias (7.30%) were common sites for malignancies in females. The higher incidence of genital and skin tumours is discussed in the paper. Available data is compared with data from Tata Memorial Cancer Hospital Bombay and Cancer Hospital at Barshi in Solapur District of Maharashtra.

Physiological pharmacokinetic and pharmacodynamic model of physostigmine in the rat.

A physiological model for physostigmine disposition was developed in the rat which incorporated anatomical, physiological, and biochemical parameters, i.e. tissue volume, plasma flow rates, drug metabolism, and tissue-to-plasma partition coefficients. Predicted concentrations of physostigmine in different tissue compartments were consistent with the experimental observations in the rat following an iv dose. Part of this study also compared the time course changes in measured effect, as percentage change in cholinesterase activity in brain, and related these changes to the plasma or brain drug level in either a combined pharmacokinetic-pharmacodynamic (plasma physostigmine-effect relationship) or a dynamic model (brain physostigmine-effect relationship). Fitting the time course of the effect in a pharmacokinetic-pharmacodynamic model required an effect compartment with the equilibration rate constant between it and the plasma compartment. Both models help to understand whether the cholinesterase activity is homogeneous or heterogenous in the brain.

Pharmacokinetics and pharmacodynamics of physostigmine in the rat after intravenous administration.

The time course of physostigmine (Phy) and metabolites in plasma, brain, and muscle, the inhibition of butyrylcholinesterase (BuChE) in plasma, and cholinesterase (ChE) activity in brain and muscle were studied in rat after iv bolus administration of 3H-Phy (100 micrograms/kg). The semilogarithmic plot of plasma Phy concentration versus time indicates a biphasic decline. These data were analyzed by nonlinear computer fitting program (PC-NONLIN) using a two-compartment open model with bolus input and first order elimination. The pharmacokinetic constants A, B, alpha, beta, AUC, K10 half-life, alpha-half-life, beta-half-life, K10, K12, and K21 were obtained. The alpha-half-life and the beta-half-life were 1.31 and 15.01 min, respectively. The apparent volume of distribution was found to be 270 ml. The clearance was 12.43 ml min-1. The half-life of Phy in brain was 11 min. The brain to plasma ratio (1.69) peaked at 15 min. Phy is metabolized to eseroline and three other metabolites, M1, M2, and M3. The distribution studies showed that the radioactivity per g of tissue was highest in kidney and liver, whereas the percentage of the administered dose in terms of radioactivity was maximum in muscle followed by liver. The maximum inhibition of BuChE (52%) correlates with the highest Phy concentration (84.6 ng/ml) in plasma at 2 min and 70% of the enzymic activity recovered by 45 min. The maximum inhibition of ChE (63%) in the brain correlates with the highest Phy concentration (128 ng/g) at 3 min, and 85% of the enzymic activity was recovered within an hour.(ABSTRACT TRUNCATED AT 250 WORDS)

The adrenal and renal veins of man and their connections with azygos and lumbar veins.

There exist many variations in the manner of formation, dimensions and place of termination of the central adrenal veins. In addition, some superficial adrenal veins may be substantial in size and may themselves drain directly into the renal veins and/or into other vessels which communicate with azygos and lumbar veins. This provides a route for venous adrenal blood to the heart via the azygos system and the superior vena cava rather than via the inferior vena cava. Variations in the formation and disposition of the renal veins are also described and the patterns of communication between somatic veins (including veins of the azygos and lumbar systems) and the left renal vein are illustrated and discussed in relation to the findings of others.

Variations in the composition of the human rectus sheath: a study of the anterior abdominal wall.

Three modes of construction of the rectus sheath above the arcuate line were found in a study of 40 cadavers. The classical description occurred most frequently, in 24 cases. The other constructions conformed to those described in earlier studies although the incidences were different. The accessory internal oblique muscle was found in none of the 40 cadavers, and pyramidalis muscle occurred on one or both both sides in only 3 out of 56 specimens. The shape and position of the arcuate line were neither symmetrical nor constant, and neither was the arrangement of the nerve supply to the rectus abdominis muscle or to the overlying skin.

Distribution and pharmacokinetics of physostigmine in rat after intramuscular administration.

The distribution and pharmacokinetics of [3H]physostigmine (Phy) and the relationship between the time course of Phy concentration and butyrylcholinesterase (BuChE) inhibition in plasma was studied in rat after im administration (650 micrograms/kg). The concentrations of Phy and its metabolites were determined in plasma and brain by high-performance liquid chromatography and by counting the radioactivity in the chromatographic fractions. The half-life of Phy in plasma and brain was 17 and 16 min, respectively. The brain-to-plasma ratio of Phy peaked (1.61) at 22 min. The time course of Phy and its metabolites (eseroline, M1 and M2) indicated that Phy was rapidly metabolized and M1 appeared to be the major metabolite. The distribution studies showed that the concentration of radioactivity per gram of tissue was higher in kidney and liver than the other tissues. The time course of BuChE activity and plasma Phy concentration showed that the maximum enzymatic inhibition (47%) occurred at about the same time (7 min) as the peak plasma concentration (583 ng/ml at 5 min). The enzymatic activity recovered to 81% at 2 hr and 100% within 24 hr.

Determination of physostigmine in plasma and brain by HPLC.

A specific, reliable, and accurate high performance liquid chromatographic method is described for the determination of physostigmine in plasma and brain using carbaryl as an internal standard. Plasma and brain containing physostigmine were first precipitated with TCA, and then carbaryl was added. This was followed by chloroform extraction and then evaporation. The residue was reconstituted in the mobile phase. Physostigmine, its hydrolyzed product eseroline, and carbaryl were separated on a reversed-phase column eluted with mobile phase containing octanesulfonic acid with phosphate buffer in a methanol and water solution. The eluted compounds were detected at 245 nm, and physostigmine was quantified from the ratio of the area of physostigmine to carbaryl peaks. The chromatography was complete within 15 min. The dynamic range of quantitation of physostigmine was 0.05 micrograms to 0.5 micrograms/mL plasma or per gram of brain. Analytical recoveries varied from 95 to 107% over this range. Coefficient of variation ranged from 1.7 to 9.5%. This method was applied to study plasma and brain concentration in rats after 650 micrograms/kg intramuscular administration of physostigmine. The ratio of brain to plasma was found to be 0.48 and 1.97 at 15 and 30 min, respectively.