ABSTRACT
Although various pharmacopoeias provide titration methods to assay (L)-amino acid content, none of these methods distinguish between (L)- and (D)-amino acids and do not consider the presence of enantiomeric impurities. Consequently, these methods are limited in scope to describe the relationship between content and specific rotation, [α]. In this study, the US Pharmacopoeia method was compared with the crown ether-based high performance liquid chromatographic (HPLC) chiral stationary phase (CSP) method to determine (L)-amino acid content and specific rotation. The (L)-amino acid content specified by the US Pharmacopoeia method was not consistent with the specific rotation in the presence of enantiomeric impurities, whereas the HPLC-CSP method was very effective for determining the (L)-amino acid content and the optical purity. The other advantage is that the HPLC-CSP method requires amino acid samples of quite low concentration (as low as 1 µg/mL), whereas the pharmacopoeia method requires higher concentrations (20-110 mg/mL).
Subject(s)
Amino Acids/chemistry , Chromatography, High Pressure Liquid/methods , Chromatography, High Pressure Liquid/instrumentation , Crown Ethers/chemistry , StereoisomerismABSTRACT
In the title compound, C(15)H(12)ClN(3)O, the quinoline ring system is essentially planar, with a maximum deviation of 0.017â (1)â Å. The crystal packing is stabilized by π-π stacking inter-actions between the quinoline rings of adjacent mol-ecule, with a centroid-centroid distance of 3.5913â (8)â Å. A weak C-Hâ¯π contact is also observed between mol-ecules.
ABSTRACT
In the title compound, C(16)H(13)ClN(2)O, the quinoline ring system is approximately planar [maximum deviation 0.021â (2)â Å] and forms a dihedral angle of 85.93â (6)° with the pyridone ring. Inter-molecular C-Hâ¯O hydrogen bonding, together with weak C-Hâ¯π and π-π inter-actions [centroid-to-centroid distances 3.5533â (9) and 3.7793â (9)â Å], characterize the crystal structure.
ABSTRACT
In the title mol-ecule, C(21)H(15)ClN(4)S, the triazoloisoquinoline ring system is approximately planar, with an r.m.s. deviation of 0.054â (2)â Å and a maximum deviation of 0.098â (2)â Å from the mean plane for the triazole ring C atom that is bonded to the thia-zole ring. The thia-zole and benzene rings are twisted by 66.36â (7) and 56.32â (7)°, respectively, with respect to the mean plane of the triazoloisoquinoline ring system. In the crystal structure, mol-ecules are linked by inter-molecular C-Hâ¯N inter-actions along the a axis. The mol-ecular conformation is stabilized by a weak intra-molecular π-π inter-action involving the thia-zole and benzene rings, with a centroid-centroid distance of 3.6546â (11)â Å. In addition, two other intermolecular π-π stacking inter-actions are observed, between the triazole and benzene rings and between the dihydro-pyridine and benzene rings [centroid-centroid distances = 3.6489â (11) and 3.5967â (10)â Å, respectively].
ABSTRACT
In the title mol-ecule, C(20)H(12)ClN(3)O, the triazoloisoquinoline ring system is nearly planar, with an r.m.s. deviation of 0.018â (3)â Å and a maximum deviation of 0.034â (3)â Å from the mean plane for the triazole ring C atom which is bonded to the benzene ring. The furan and benzene rings are twisted by 59.71â (14) and 66.95â (10)°, respectively, with respect to the mean plane of the triazoloisoquinoline ring system. The mol-ecular conformation is stabilized by an intra-molecular π-π inter-action [centroid-to-centroid distance = 3.5262â (18)â Å]. The crystal packing is stabilized by weak C-Hâ¯π inter-actions and weak π-π inter-actions [centroid-to-centroid distance = 3.9431â (17)â Å].
ABSTRACT
In the title mol-ecule, C(23)H(14)N(4), the triazoloisoquinoline ring system is nearly planar, with an r.m.s. deviation of 0.038â (2)â Å and a maximum deviation of -0.030â (2)â Å from the mean plane of the triazole ring C atom which is bonded to the benzene ring. The benzene and phenyl rings are twisted by 57.65â (8) and 53.60â (9)°, respectively, with respect to the mean plane of the triazoloisoquinoline ring system. In the crystal structure, mol-ecules are linked by weak aromatic π-π inter-actions [centroid-centroid distance = 3.8074â (12)â Å]. In addition, the crystal structure exhibits a nonclassical inter-molecular C-Hâ¯N hydrogen bond.
ABSTRACT
In the title mol-ecule, C(22)H(14)ClN(3), the triazoloisoquinoline ring system is approximately planar, with an r.m.s. deviation of 0.033â (2)â Å and a maximum departure from the mean plane of 0.062â (1)â Å for the triazole ring C atom, bonded to the benzene ring. The benzene and phenyl rings are twisted by 57.02â (6) and 62.16â (6)°, respectively, to the mean plane of the triazoloisoquinoline ring system. The mol-ecule is stabilized by a weak intra-molecular π-π inter-action [centroid-centroid distance = 3.7089â (10)â Å] between the benzene and phenyl rings. In the crystal structure, weak inter-molecular C-Hâ¯N hydrogen bonds and C-Hâ¯π inter-actions link the mol-ecules.
ABSTRACT
In the title compound, C(18)H(11)ClN(2)O(2), the isatin and 2-chloro-3-methyl-quinoline units are both almost planar, with r.m.s. deviations of 0.0075 and 0.0086â Å, respectively, and the dihedral angle between the mean planes of the two units is 83.13â (7)°. In the crystal, a weak inter-molecular C-H⯠O inter-action links the mol-ecules into chains along the c axis.
ABSTRACT
In the title compound, C(15)H(11)Cl(2)NO(2), the dihedral angle between the two benzene rings is 74.83â (5)°. The N-bound and terminal benzene rings are inclined at dihedral angles of 4.09â (10) and 78.38â (9)°, respectively, to the mean plane through the acetamide group. Intra-molecular C-Hâ¯O and N-Hâ¯O hydrogen bonds both generate S(6) rings.
ABSTRACT
The title mol-ecule, C(5)H(7)N(3)O(2), has an almost planar conformation, with a maximum deviation of 0.043â (3)â Å, except for the methyl H atoms. In the crystal structure, inter-molecular C-Hâ¯O hydrogen bonds link the mol-ecules into layers parallel to the bc plane. Inter-molecular π-π stacking inter-actions [centroid-centroid distances = 3.685â (2) and 3.697â (2)â Å] are observed between the parallel triazole rings.
ABSTRACT
The title compound, C(13)H(14)OS, crystallizes with two independent mol-ecules in the asymmetric unit. The unit cell contains three voids of 197â Å(3), but the residual electron density (highest peak = 0.24â eâ Å(-3) and deepest hole = -0.18â eâ Å(-3)) in the difference Fourier map suggests no solvent mol-ecule occupies this void. The crystal structure is stabilized by π-π inter-actions between the isocoumarin ring systems, with centroid-centroid distances of 3.6793â (14) and 3.6566â (15)â Å.
ABSTRACT
The title compound, C(25)H(19)N(3), is composed of an aryl-substituted pyrazole ring connected to an aryl-substituted isoquinoline ring system with a dihedral angle of 52.7â (1)° between the pyrazole ring and the isoquinoline ring system. The dihedral angle between the pyrazole ring and the phenyl ring attached to it is 27.4â (1)° and the dihedral angle between the isoquinoline ring system and the phenyl ring attached to it is 19.6â (1)°.
ABSTRACT
The title compound, C(30)H(20)ClN(3), is composed of a diaryl-substituted pyrazole ring connected to an aryl-substituted isoquinoline ring system with a dihedral angle of 65.1â (1)° between the pyrazole ring and the isoquinoline ring system. The 3-phenyl and 4-phenyl substitutents are twisted by 8.1â (1) and 43.0â (1)°, respectively, with respect to the pyrazole ring. The chloro-phenyl ring and the isoquinoline ring system are twisted by 21.2â (1)° with respect to each other.
ABSTRACT
The title compound, C(22)H(20)ClN(3), is composed of a dialkyl-substituted pyrazole ring connected to an aryl-substituted isoquinoline ring system with a dihedral angle of 55.8â (1)° between the pyrazole ring and and the isoquinoline ring system. The dihedral angle between the chloro-phenyl ring and the isoquinoline ring system is 28.3â (1)°.
ABSTRACT
The mol-ecular conformation of the title compound, C(19)H(18)O(2), is stabilized by an intra-molecular O-H-O hydrogen bond. In addition, inter-molecular O-H-O inter-actions link the mol-ecules into zigzag chains running along the c axis.
ABSTRACT
All the non-H atoms of the title compound, C(10)H(11)NO(2), are almost coplanar [maximum deviation = 0.040â (3)â Å]. The crystal structure is stabilized by C-Hâ¯O hydrogen bonds.
ABSTRACT
In the title mol-ecule, C(20)H(13)N(3)S, the triazoloisoquinoline ring system is approximately planar, with an r.m.s. deviation of 0.045â Å and a maximum deviation of 0.090â (2)â Å from the mean plane for the triazole ring C atom which is bonded to the thio-phene ring. The phenyl ring is twisted by 52.0â (1)° with respect to the mean plane of the triazoloisoquinoline ring system. The thio-phene ring is rotationally disordered by approximately 180° over two sites, the ratio of refined occupancies being 0.73â (1):0.27â (1).
ABSTRACT
In the title compound, C(17)H(15)ClN(2)O, the quinoline ring system is nearly planar, with a maximum deviation from the mean plane of 0.074â (2)â Å, and makes a dihedral angle of 81.03â (7)° with the pyridone ring. The crystal packing is stabilized by π-π stacking inter-actions between the pyridone and benzene rings of the quinoline ring system [centroid-centroid distance = 3.6754â (10)â Å]. Furthermore, weak inter-molecular C-Hâ¯O hydrogen bonding links mol-ecules into supra-molecular chains along [001].
ABSTRACT
In the title mol-ecule, C(14)H(10)ClNO, all non-H atoms are coplanar (r.m.s deviation = 0.0266â Å). In the crystal, symmetry-related mol-ecules are hydrogen bonded via inter-molecular O-Hâ¯O inter-actions, forming chains along the b axis.
ABSTRACT
In the title compound, C(16)H(13)ClN(2)O, the quinoline ring system is essentially planar, with a maximum deviation of 0.021â (2)â Å. The pyridone ring is oriented at a dihedral angle of 85.93â (6)° with respect to the quinoline ring system. In the crystal structure, inter-molecular C-Hâ¯O hydrogen bonds link the mol-ecules along the b axis. Weak π-π stacking inter-actions [centroid-centroid distances = 3.7218â (9) and 3.6083â (9)â Å] are also observed.