ABSTRACT
The urgency of the problem of coxyellosis in children is determined by the endemic nature of this pathology for a number of regions of Russia. The purpose of the study: to evaluate the results of diagnosis of coxyellosis in children using the methods of complement binding reaction (RSC), enzyme immunoassay (ELISA), and polymerase chain reaction (PCR). Retrospective analysis of the survey on Coxiella in 3 groups of children aged 7 to 17 years: group 1 (n=30) method RSK; group 2 (n=34) - by ELISA; group 3 (n=35) - PCR, were hospitalized in GBUZ «Regional clinical infectious hospital named. A. M. Nicholi¼ Astrakhan in the period from January 2010 to January 2020. The most informative methods of diagnosis of coxyellosis in children during the first 7 days from the onset of the disease is the PCR reaction (specificity-94%, sensitivity-91%), after the 7th day of the disease ELISA (specificity -91%, sensitivity - 94%).The sensitivity of the RSC method is 70%, the specificity is 87%.
Subject(s)
Clinical Laboratory Techniques , Gram-Negative Bacterial Infections/diagnosis , Adolescent , Child , Coxiella , Enzyme-Linked Immunosorbent Assay , Humans , Polymerase Chain Reaction , Retrospective Studies , Russia , Sensitivity and SpecificityABSTRACT
Giardiasis in children remains an urgent problem, the importance of which is determined by the endemicity for many countries and regions, polymorphism of the clinic, which causes difficulties in clinical diagnosis of this pathology. The report presents the results of the diagnostic significance of methods of laboratory diagnostics giardiase: microscopy of native and stained with Lugol solution preparation fecal enzyme-linked immunoassay (ELISA) for the presence of antibodies of class IgM and IgG antibodies to the antigens of Giardia in the serum and antigen of G. Lamblia in feces, polymerase chain reaction (PCR) - detection of Giardia DNA in feces obtained during the examination of 160 patients with giardiasis children aged 3 to 14 years. The results of our study suggest that the most informative methods of diagnosis of giardiasis in children is coproscopy (specificity - 96.2%, sensitivity - 98.1%), PCR coprofiltrate. (specificity - 85%, sensitivity 82.5%), determination of antigen in feces by ELISA (specificity - 87,5%, sensitivity - 60%). With high specificity of the blood ELISA method (90%), the sensitivity of the method is only 27.5%. It should be recognized that the «gold standard¼ diagnosis of giardiasis remains microscopy of native and stained with a solution of Lugol preparation of feces with a three - time study at intervals of 2-3 days.
Subject(s)
Giardia lamblia/isolation & purification , Giardiasis/diagnosis , Adolescent , Antibodies, Protozoan/blood , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Feces/parasitology , Humans , Microscopy , Polymerase Chain Reaction , Sensitivity and SpecificityABSTRACT
Cytochemical indices of peripheral blood leukocytes were investigated. The results allowed to estimate the level of the disease severity and to control the immunotropic therapy efficacy. Dysbalance of the interleukocytic microbicidical systems (IMS) of neutrophils reflected the nonspecific resisistance and the state of the phagocytic system as an objective criterion of the adaptation syndrome. Association of the deficiency of the IMS components and blood serum fibronectin were considered as a predisposition of bacterial complications. Cycloferon activated the phagocyte metabolic activity and promoted elimination of the main clinical symptoms of the disease (acute infective damage of the meninges, temperature elevation, intoxication signs), thus making shorter the hospitalization terms.
Subject(s)
Acridines/administration & dosage , Arbovirus Infections , Immunotherapy , Interferon Inducers/administration & dosage , Adolescent , Arbovirus Infections/blood , Arbovirus Infections/immunology , Arbovirus Infections/therapy , Child , Child, Preschool , Female , Fibronectins/blood , Fibronectins/immunology , Humans , Infant , Leukocytes/immunology , Leukocytes/metabolism , MaleABSTRACT
In accordance with the results of laboratory diagnostics, carried out by bacteriological, serological and PCR methods, the etiological structure of acute bacterial and viral intestinal infections, most frequently occurring in children of the Astrakhan region, is presented. An important role of viral diarrhea cases, prevailing among the total number of cases of intestinal infections in children, is emphasized. The data on the irregular distribution of morbidity by months, especially in viral intestinal infections and salmonellosis with the prevalent involvement of children aged up to 2 years into the epidemic process, are presented. Infections affect mainly children given artificial feeding, as well as those with complicated premorbid state and frequently having acute respiratory viral infections.
Subject(s)
Enterocolitis/diagnosis , Enterocolitis/epidemiology , Acute Disease , Age Factors , Bottle Feeding , Child , Child, Preschool , Comorbidity , Dysentery, Bacillary/diagnosis , Dysentery, Bacillary/epidemiology , Enterocolitis/microbiology , Humans , Infant , Respiratory Tract Infections , Risk Factors , Russia/epidemiology , Salmonella Infections/diagnosis , Salmonella Infections/epidemiology , Virus Diseases/diagnosis , Virus Diseases/epidemiologyABSTRACT
As many as 76 children of the first year of life suffering from salmonellosis were examined for the clinical manifestations of the disease and for the immune status. The data were obtained, pointing to the development of secondary immuno- and granulocytopathies influencing the course the disease takes.
