ABSTRACT
Survival of bifidobacteria and preservation of their morphological characteristics after 12-month storage of lyophilized cells was studied for the strains of Bifidobacterium bifidum and B. animalis isolated and maintained in the microbial collection of the Department of Microbiology, Moscow State University. A combined approach to pre-lyophilization treatment of microorganisms and subsequent storage was developed in order to improve cell survival. Compared to the standard cryoprotector concentrations, sucrose and glucose (5% and higher) in skim milk, as well as freezing at â70°C with subsequent storage at the same temperature resulted in improved survival of bifidobacteria. Under such conditions, the number of viable cells (CFU) after 12 months of storage was two to three orders of magnitude higher than in the case of the standard lyophilization procedure. Investigation of dynamics of resistance of reactivated clones to such gastrointestinal stress factors as gastric juice and bile acids revealed preservation of these properties at all storage modes. However, since the number of surviving cells decreased during storage according to the standard procedure, the number of stress-affected cells was correspondingly lower. Reactivated cultures exhibited high resistance to oxygen, with survival decreasing to 35% of the initial level.
Subject(s)
Bifidobacterium animalis/growth & development , Bifidobacterium bifidum/growth & development , Microbial Viability , Preservation, Biological , Probiotics , Bifidobacterium animalis/cytology , Bifidobacterium bifidum/cytology , Freeze Drying , Time FactorsABSTRACT
Currently, no molecular biological markers do exist for early diagnosis of breast cancer. One of the possible candidates for the marker of early breast cancer is mammaglobin (MGB1) or SCGB2A2 (secretoglobin, family 2A, member 2), characterized by the maximal expression level in early breast cancer. Using the RT-PCR method MGB1 mRNA expression was examined in 57 tumor tissue samples and 57 samples of morphologically non-malignant tissue (MNT) of breast cancer (BC) patients. Specificity and sensitivity of the MGB1 mRNA assay in peripheral blood of BC patients was evaluated by nested PCR. 169 blood samples (from 95 BC patients, 22 from patients with benign breast tumors, 28 from patients with tumors of other localizations, and 24 samples from healthy donors) have been analyzed. MGB1 expression was significantly higher in BC tissue samples compared to MNT (p=0.0019). The maximal expression level was in the samples T1 (p=0.013), stage I BC (p=0.037), GI (p=0.0019). The MGB1 expression positively correlated with expression of estrogen (p = 0,034) and progesterone (p=0.0004) receptors. Sensitivity and specificity of the MGB1 mRNA assay in peripheral blood were 60.6% and 92.3%, respectively. Expression of MGB1 was higher in BC than MNT and it decreased during BC progression. The sensitivity and specificity of the MGB1 mRNA assay may be used as an additional diagnostic method.
Subject(s)
Biomarkers, Tumor/blood , Breast Neoplasms/blood , Mammaglobin A/blood , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolism , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Case-Control Studies , Female , Humans , Mammaglobin A/genetics , Mammaglobin A/metabolism , Middle Aged , RNA, Messenger/blood , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Estrogen/genetics , Receptors, Estrogen/metabolism , Receptors, Progesterone/genetics , Receptors, Progesterone/metabolismABSTRACT
The study showed the results of univariate and multivariate analyses of treatment of patients with low-grade brain supra- tentorial infiltrative gliomas. It was reaffirmed the importance of the most significance of such adverse prognostic factors as histology, tumor size, displacement of midline structures of the brain, age and neurological deficit. When assessing the prognostic significance of severity of the debut of clinical symptoms in this category of neuro-oncological patients as well as radical surgical treatment of the primary tumor there were obtained data confirming the absence of their independent prognostic significance.
Subject(s)
Brain Neoplasms/diagnosis , Brain Neoplasms/mortality , Glioma/diagnosis , Glioma/mortality , Adult , Brain Neoplasms/therapy , Disease-Free Survival , Female , Glioma/therapy , Humans , Male , Survival RateABSTRACT
Six stably growing strains of bifidobacteria possessing probiotic properties were isolated from the feces of newborn children and animals. According to the results of molecular analysis, one strain was classified as Bifidobacterium bifidum, while five belonged to Bifidobacterium animalis. Initial identification of the strains was carried out using the primer pairs for the 16S rRNA gene (g-Bifid-F/R, Bif164/662) and for the xfp gene specific for bifidobacteria. Subsequent sequencing of complete genes encoding 16S rRNA synthesis in the isolates confirmed their species affiliation. The cultures exhibited high resistance to gastroenterological stress (5 ≤ RD ≤ 10) and may be therefore recommended as:potential probiotics.
Subject(s)
Bifidobacterium/isolation & purification , Genes, Bacterial , Probiotics/isolation & purification , RNA, Ribosomal, 16S/genetics , Adaptation, Physiological , Animals , Animals, Newborn , Bifidobacterium/drug effects , Bifidobacterium/genetics , Biomimetic Materials/chemistry , Biomimetic Materials/pharmacology , Feces/microbiology , Gastric Juice/chemistry , Humans , Infant, Newborn , Sequence Analysis, DNAABSTRACT
The biological effect of the extracellular peptide reactivating factor (RF) from Luteococcus casei on cells of probiotic cultures was studied. The RF showed the protective and reactivating effects on the Bifidobacterium bifidum cells under the action of bile salts and an acidic stress. Also, it acted as a cryoprotector during lyophilisation and long-term culture storage. The RF and the L. casei culture liquid (CL) were shown to have bifidogenic properties. The degree of protection and reactivation of lactic-acid bacteria under the action of bile salts depended on the particular strain properties. The maximum degree of protection (more than thirteen-fold) and reactivation (close to three-fold) was found in Lactobacillus casei, while the minimum values were characteristic of Lactobacillus reuterii. The resistance of lactobacilli to bile was increased in the row of L. acidophilus, L. casei, L. plantarum, L. rhamnosus, and L. reuterii correlating with the RF protection degree.
Subject(s)
Bacterial Proteins/pharmacology , Bifidobacterium/drug effects , Bile Acids and Salts/pharmacology , Cryoprotective Agents/pharmacology , Lacticaseibacillus casei/metabolism , Bacterial Proteins/metabolism , Bifidobacterium/growth & development , Bile Acids and Salts/antagonists & inhibitors , Cryoprotective Agents/metabolism , Freeze Drying , Lactobacillus acidophilus/metabolism , Lactobacillus plantarum/metabolism , Limosilactobacillus reuteri/metabolism , Lacticaseibacillus rhamnosus/metabolism , Probiotics , Species Specificity , Stress, PhysiologicalABSTRACT
The results of complex neurological and neuropsychological study with evaluation of anxiety levels in young and middle-age patients with chronic hepatitis and liver cirrhosis are presented in the article. Raised anxiety levels with statistically significant difference to control group patients were found. By turn, anxiety levels in patients with chronic hepatitis were lower than in patients with liver cirrhosis. The most of examined patients had middle (with tendency to low level as well as with tendency to high level) and high level of anxiety.
Subject(s)
Anxiety/psychology , Hepatitis, Chronic/psychology , Liver Cirrhosis/psychology , Adult , Age Factors , Anxiety/epidemiology , Case-Control Studies , Hepatitis, Chronic/epidemiology , Humans , Liver Cirrhosis/epidemiology , Middle Aged , Neuropsychological TestsABSTRACT
We used combinations of taxan-based neoadjuvant and adjuvant full-dose chemotherapy and aggressive combined radiotherapy including clinical target volume extension, increased total dosage, hyperthermia, cryo- and local chemotherapy as radiosensitizers, for treatment of invasive and locally-advanced breast cancer or endometrial carcinoma with poor prognosis. 3D-ultrasound/CT/MRI--based designing of radiotherapy and monitoring of dynamic definition of target volume and "high risk volume" in organs at risk in cases of tumor progression was an indispensable measure. As a result, no local recurrence was reported in 73% for 36 months.
Subject(s)
Breast Neoplasms/radiotherapy , Carcinoma/radiotherapy , Endometrial Neoplasms/radiotherapy , Neoadjuvant Therapy/methods , Neoplasm Recurrence, Local/prevention & control , Uterine Cervical Neoplasms/radiotherapy , Adult , Aged , Antineoplastic Agents/administration & dosage , Breast Neoplasms/pathology , Breast Neoplasms/therapy , Carcinoma/secondary , Carcinoma/therapy , Chemotherapy, Adjuvant , Cryotherapy , Endometrial Neoplasms/pathology , Endometrial Neoplasms/therapy , Female , Humans , Hyperthermia, Induced , Imaging, Three-Dimensional , Magnetic Resonance Imaging , Middle Aged , Neoplasm Staging , Prognosis , Radiation-Sensitizing Agents/therapeutic use , Radiotherapy Dosage , Radiotherapy, Adjuvant , Risk Assessment , Tomography, X-Ray Computed , Treatment Outcome , Ultrasonography , Uterine Cervical Neoplasms/pathology , Uterine Cervical Neoplasms/therapyABSTRACT
Non-steroid antiinflammatory drug (NSAID)-related gastropathy is regarded as the most important NSAID-induced complication. The above term is used to signify erosive and ulcerous injuries to the stomach and duodenum, having a characteristic clinical picture.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/adverse effects , Gastric Mucosa/drug effects , Intestinal Mucosa/drug effects , Stomach Diseases/chemically induced , Helicobacter Infections/complications , Helicobacter pylori , Humans , Peptic Ulcer/complicationsABSTRACT
The nosologic and etiopathogenetic structure of the cholestatic syndrome encountered both in surgical and therapeutical practice is described.
Subject(s)
Cholestasis/drug therapy , Cholestasis/metabolism , Hepatitis B, Chronic/metabolism , Hepatitis, Alcoholic/metabolism , Liver Cirrhosis, Biliary/metabolism , Alkaline Phosphatase/blood , Bilirubin/blood , Cholagogues and Choleretics/therapeutic use , Cholestasis/etiology , Cholesterol/blood , Hepatitis B, Chronic/complications , Hepatitis, Alcoholic/complications , Humans , Lipoproteins/blood , Liver Cirrhosis, Biliary/complications , Phenobarbital/therapeutic use , Phospholipids/blood , Triglycerides/blood , Ursodeoxycholic Acid/therapeutic use , gamma-Glutamyltransferase/bloodABSTRACT
Results are submitted of bacteriological investigations in those persons with duodenal peptic ulcer who had been called up. The presence of regional strains of H. pylori is ascertained together with their sensitivity to certain antibacterial drug preparations. A rational antihelocobactertherapy schedule is proposed.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Anti-Ulcer Agents/therapeutic use , Duodenal Ulcer/drug therapy , Helicobacter pylori/drug effects , Peptic Ulcer/drug therapy , Adolescent , Anti-Bacterial Agents/pharmacology , Anti-Ulcer Agents/pharmacology , Duodenal Ulcer/diagnosis , Duodenal Ulcer/microbiology , Helicobacter pylori/isolation & purification , Humans , Interviews as Topic , Male , Peptic Ulcer/diagnosis , Peptic Ulcer/microbiology , Treatment Outcome , Ukraine/epidemiologyABSTRACT
The chief factors of aggression are discussed together with mechanisms of protection of gastric and duodenal mucosa. Results of treatment of 30 patients with gastritis (Helicobacter pylori negative) with a cytoprotective drug smecta are presented.
Subject(s)
Antidiarrheals/therapeutic use , Cytoprotection , Intestinal Mucosa/drug effects , Silicates/therapeutic use , Adolescent , Adult , Aged , Female , Gastritis/drug therapy , Gastritis/etiology , Gastritis/pathology , Humans , Intestinal Mucosa/pathology , Male , Middle Aged , Stress, PhysiologicalABSTRACT
Modern definition, classifications, diagnosis and treatment of irritable bowel syndrome (IBS) are presented together with results of treatment of 30 patients with irritable bowel syndrome with the new selective calcium-channel blocker dicetel.
Subject(s)
Calcium Channel Blockers/therapeutic use , Colonic Diseases, Functional/diagnosis , Colonic Diseases, Functional/drug therapy , Morpholines/therapeutic use , Adult , Colonic Diseases, Functional/classification , Female , Humans , Male , Middle Aged , Treatment OutcomeABSTRACT
Peptic ulcer (PU) is one of the most prevalent medical problems. It is essential for PU patients to undergo gastrofibroscopy, pH-metry, and examination for Helicobacter pylori during the phase of exacerbation. Results of a comprehensive evaluation of patients will, we believe, help in ensuring the chosen treatment schedule compliance for each particular patient. Use of amoxycillin, metronidazole, and bismuth combined with raniberl in treatment of the above category of patients appeared to be an effective therapeutic alternative designed to eradicate Helicobacter pylori.
Subject(s)
Helicobacter Infections/drug therapy , Helicobacter pylori , Peptic Ulcer/drug therapy , Anti-Bacterial Agents/adverse effects , Anti-Bacterial Agents/therapeutic use , Anti-Ulcer Agents/adverse effects , Anti-Ulcer Agents/therapeutic use , Drug Therapy, Combination , Helicobacter Infections/etiology , Humans , Peptic Ulcer/complications , Proton Pump Inhibitors , Time FactorsABSTRACT
Our objective in this study was investigation into the effectiveness of the enzymic preparation Kirshner Pancreal in 27 patients with chronic pancreatitis. It is shown that against the background of treatment with the above drug patients dispelled the pain syndrome and manifestations of the maldigestion syndrome. The described drug made for normalization of the blood serum content of beta-lipoproteins. The drug is well-tolerated by patients, no adverse effects were noted.
Subject(s)
Enzyme Therapy , Pancreatitis/drug therapy , Chronic Disease , Combined Modality Therapy , Drug Combinations , Enzymes/adverse effects , Female , Humans , Male , Pancreatitis/diagnosis , Tablets , Time FactorsABSTRACT
Osteodensity was compared in 582 postmenopausal patients with such pathologies as urogenital atrophy, osteoporosis, vulvar craurosis, breast carcinoma, hyperplasia and endometrial carcinoma. A double-photon Z-ray densitometer with a computer program designed for postmenopausal women examination was employed. It was shown that osteodensity measurements can distinguish two opposite patterns of homeostasis in postmenopause: involute-atrophic vs. hyperplastic processes in target tissues characterized by high oncological susceptibility. However, if strict and clear distinctions are applied, many patterns of pathology combination so typical of postmenopause will stay outside the classification. Much in further studies of the problem will depend on progress made in investigation of estrogen receptors in target tissues.
Subject(s)
Absorptiometry, Photon , Bone Density , Bone and Bones/diagnostic imaging , Osteoporosis, Postmenopausal/diagnostic imaging , Postmenopause , Bone and Bones/pathology , Female , Humans , Middle Aged , Population SurveillanceABSTRACT
Findings on sonography diagnostic potential to be used in screening for endometrial carcinoma suggested an ultrasound examination of 235 postmenopausal patients who had had no relevant complaints. As a result, criteria of ultrasound screening for endometrial tumors in postmenopausal patients were worked out. Later, sonography was carried out in 596 endometrial carcinoma patients with a view to verify the diagnosis. It pointed to inadequate identification of microinvasive carcinoma (up to 5 mm) by transabdominal and transvaginal scanning. Sonography, however, efficiently diagnosed marked infiltration (9.4%), organ involvement (88.4%) and metastatic lesions in the ovaries.
Subject(s)
Carcinoma/diagnostic imaging , Endometrial Neoplasms/diagnostic imaging , Mass Screening/methods , Abdomen , Aged , Carcinoma/prevention & control , Carcinoma/secondary , Diagnosis, Differential , Endometrial Neoplasms/pathology , Endometrial Neoplasms/prevention & control , Female , Humans , Middle Aged , Ovarian Neoplasms/diagnostic imaging , Ovarian Neoplasms/secondary , Ultrasonography/methods , VaginaABSTRACT
The study was concerned with radiotherapy planning using individual data on tissue volume to be treated. The investigation used criteria based on correlations of TDF, actual volume identified by ultrasound, computed and magnetic resonance tomography and dose fractionation regime. As a result, grave complication rates dropped 3 times including cases of locally advanced tumors, non-traditional dose fractionation and advanced regimens of polychemotherapy.
Subject(s)
Ovarian Neoplasms/radiotherapy , Radiation Injuries/prevention & control , Radiotherapy, Computer-Assisted/methods , Uterine Neoplasms/radiotherapy , Dose Fractionation, Radiation , Female , Humans , Radiation Injuries/etiology , Radiotherapy/adverse effects , Radiotherapy Planning, Computer-Assisted/methods , Treatment OutcomeABSTRACT
A classification is submitted of cholelithiasis, taking account of stages of the illness, its main clinical forms and complications, with the diagnostic criteria and policy of treating it being pointed out. The use of the above classification ensures succession of surgeons' and therapists' work in questions of diagnosis, prophylaxis, and treatment of patients with cholelithiasis.