ABSTRACT
Adult Astiotrema odhneri Bhalerao, 1936 sensu Cho & Seo 1977 were found in the intestine of a freshwater turtle, Pelodiscus sinensis (Wiegmann), from the Komissarovka River Basin, Primorsky Region, Russia. It was established that the first intermediate host of this parasite is a snail, Anisus centrifugops, and that the second intermediate hosts include the snails, Helicorbis sujfunensis and A. centrifugops, tadpoles of the frog Rana dybowskii, and the fish Perccottus glenii. The development of A. odhneri includes the formation of sporocyst and xiphidiocercariae, which is typical for species belonging to Plagiorchioidea. Phylogenetic analysis based on 28S rRNA gene sequences showed that A. odhneri, together with Astiotrema monticellii, form a monophyletic clade that was closer to Opisthorchioidea than to any other taxon represented in the tree. However, phylogenetic analysis without outgroup taxon indicated a high degree of differentiation of Astiotrema from both Plagiorchioidea and Opisthorchioidea.
Subject(s)
Intestines/parasitology , Life Cycle Stages/physiology , Trematoda/classification , Trematoda/growth & development , Turtles/parasitology , Animals , Larva/parasitology , Oocysts/physiology , Perciformes/parasitology , Phylogeny , RNA, Ribosomal, 28S/genetics , Ranidae/parasitology , Rivers/parasitology , Russia , Snails/parasitology , Trematoda/anatomy & histology , Trematoda/geneticsABSTRACT
We studied adsorption capacity of 5 latexes to synthetic peptide fragments of mu- and delta-opioid receptors and to GluR1 and NR2A subunits of glutamate receptor. Levels of autoantibodies to opioid receptors in the latex agglutination test and enzyme immunoassay were in good correlation. The level of autoantibodies to opioid receptors measured by these methods was increased in patients with opium narcomania, while the content of autoantibodies to the glutamate receptor subunits was increased in epileptics.
Subject(s)
Autoantibodies/blood , Epilepsy/diagnosis , Latex Fixation Tests , Opioid-Related Disorders/diagnosis , Receptors, AMPA/immunology , Receptors, N-Methyl-D-Aspartate/immunology , Receptors, Opioid/immunology , Humans , Immunoenzyme Techniques , Latex/chemistry , Latex/immunologyABSTRACT
Adsorption ability of few kinds of latex covered by synthetic peptide fragments of mu- and delta-opiate receptors (OR) is investigated. The levels of autoantibodies to opiate receptors fragments in the blood serum of patients with drug abuse are detected by latex agglutination and ELISA. The patients with drug abuse demonstrated positive latex agglutination reaction for level specific antibodies from 10.4 mg/ml and higher in the 71.4% of cases. The levels of autoantibodies to OR in the blood of patients with drug abuse was in 2.8 times higher of control data. The correlation between levels of autoantibodies to opiate receptors obtained by methods of latex agglutination and ELISA is revealed. The obtained data confirms our hypothesis concerning existence of specific changes in immune system linked with some CNS disorders like drug abuse. Thus, the level of autoantibodies to opiate receptors could be used as new criterion for diagnostics of opiate abuse.
