ABSTRACT
BACKGROUND: The RBCs storage lesion is most carefully viewed as the sum of all the changes in RBCs occurring during the course of storage and that limit their survival. MATERIALS AND METHODS: Erythrocytes were isolated from stored blood at regular intervals. Oxidative stress markers were analyzed to determine the changes during the storage. RESULTS: Antioxidant enzymes--(SOD and CAT), and SH showed insignificant variation whereas hemolysis, MDA and AOPP showed significant variations. CONCLUSION: The oxidative stress has not successfully overridden the protection offered by the endogenous antioxidant system. Prolonged storage may result in the onset of erythrocyte deterioration. This clearly indicates that the erythrocytes are capable of attenuating ROS with 2 weeks of storage.
