Synthesis of carboxymethyl starch co (polyacrylamide/ polyacrylic acid) hydrogel for removing methylene blue dye from aqueous solution.

Natural polysaccharides, notably starch, have garnered attention for their accessibility, cost-effectiveness, and biodegradability. Modifying starch to carboxymethyl starch enhances its solubility, swelling capacity, and adsorption efficiency. This research examines the synthesis of an effective hydrogel adsorbent based on carboxymethyl starch for the elimination of methylene blue from aqueous solutions. The hydrogel was synthesized using polyacrylamide and polyacrylic acid as monomers, ammonium persulfate as the initiator, and N,N'-methylenebisacrylamide as the cross-linker. Through FESEM, swelling morphology was evaluated in both distilled water and methylene blue dye. The adsorption data elucidated that the adsorption capacity of the hydrogel significantly depends on the dosage of the adsorbent, pH, and concentration of the MB dye. At a pH of 7 and a dye concentration of 250 mg/L, the hydrogel exhibited an impressive 95 % removal rate for methylene blue. The results indicate that the adsorption process follows pseudo-second-order kinetics and conforms well to the Langmuir adsorption isotherm, indicating a maximum adsorption capacity of 1700 mg/g. According to the pseudo-second-order kinetic model and FTIR analysis, methylene blue chemisorbs to the adsorbent material. Hydrogel absorbents regulate adsorption through both intra-particle diffusion and liquid film diffusion. These results highlight the potential of the new hydrogel absorber for water purification.

A novel nanocomposite for photocatalytic rhodamine B dye removal from wastewater using visible light.

Providing safe access to water and addressing the impact of waterborne diseases, which claim over two million lives annually, is a major contribution to water purification. The study introduces a novel nanocomposite, Ch/Fe3O4/α-MoO3, which exhibits outstanding photocatalytic efficacy under visible light. An in-depth investigation of the nanocomposite's synthesis, characterization, and photodegradation mechanisms reveals its outstanding capabilities. Photocatalytic activity is influenced by the catalytic dose, pH, dye concentration, and reaction time, according to the study. A response surface method is used to determine the optimal conditions for Rhodamine B degradation, which results in 96.3% removal efficiency at pH 8.5, dye concentration 25 mg/L, nanocomposite dose at 22 mg/L, and reaction time 50 min. As a result of its high surface area, biocompatibility, availability, and magnetization with iron compounds, Chitosan is an excellent substrate for enhancing the photocatalytic properties of MoO3 nanoparticles. A nanocomposite with an energy band of 3.18 eV exhibits improved visible light absorption. This study confirms the nanocomposite's recyclability and stability, affirming its practicality. Besides dye removal, it offers hope for the global quest for clean water sources by addressing a broader range of waterborne contaminants. By combining molybdenum and magnetite, nanocomposite materials facilitate the degradation of pollutant and bacteria, contributing positively to society's quest for clean and safe water. It emphasizes the role nanotechnology plays in preserving human health and well-being in combating waterborne diseases.

Characterization of free and immobilized lipase from Penicillium sp. onto three modified bentonites: A comparative study.

The present work was conducted to investigate the immobilization of lipase from Penicillium sp. onto three modified bentonites by simple adsorption and crosslinking methods. The composites were characterized by FTIR, SEM and BET. The free and bentonite-supported lipase was evaluated in terms of operational and storage stability and pH and thermal activity and stability. The kinetic parameters were also evaluated. The results show that all immobilized enzymes had better thermal and pH stability compared to free enzymes. Among the immobilized enzymes, GDU-bent-lipase had more efficient performance in thermal (38% of its initial activity within 24 h at 65 °C), operational (70% residual activity after 9 cycles), storage stability (70.14% of its initial activities at 4 °C for 21 days), and kinetic properties (effectiveness factor 0.79 relative to free enzyme) than free and other immobilized enzymes. The adsorption isotherm was modeled by Langmuir, Freundlich and Temkin isotherms which Langmuir isotherm indicated a better fit of the experimental adsorption data. To the best of our knowledge, this is the first comparative report about the immobilization of lipase Produced by Penicillium sp., isolated from olive mill wastewater, and the most comprehensive study about the immobilization of lipase onto several supports.

Optimization of zinc bioleaching from paint sludge using Acidithiobacillus thiooxidans based on response surface methodology.

The reduction of zinc metal in the paint sludge, a hazardous waste, was investigated using Acidithiobacillus thiooxidans by a two-stage bioleaching process. This process was performed using the response surface methodology (RSM) method based on the central composite design (CCD). Four variables, a temperature range of 32-34.5-37 °C, rotation speed of shaker 120-150-180 rpm, pH of 4.2-3.2-2.2, and particle sizes of 1-2-3 mm, were used to optimize the experiments. The results showed that with a constant pulp density of 10 g/L at 32 °C, shaker speed of 120 rpm, a particle size of 1 mm and pH of 4.2, the highest removal predicted by the software (Design Expert version 11) was 22.89%. Repeating the experiments confirmed a decrease in zinc to the nearest predicted point. According to the ANOVA result, the rotation speed of the shaker has the greatest effect on the bioleaching process, followed by the two variables of the rotation speed of shaker and pH together affects. After the bioleaching process, energy dispersive X-ray (EDX) and mapping analysis showed quantitative changes in the chemical composition of the paint sludge, and morphological changes of texture were confirmed by scanning electron microscopy (SEM).

Simultaneous evaluation of the effect of mixing efficiency on power consumption and methane production in an anaerobic digester with different wastewaters.

This study simultaneously examines the effect of mixing rate on power consumption and methane production in a stirrer anaerobic reactor. The numerical simulation is carried out using the finite volume approach and validated against available experimental data. The methane production rate is determined using governing equations in the anaerobic digestion (AD) process. The results showed that a 60% increase in the mixing rate of the system (from 50% to 80%) in the wastewater with concentration of 14,549 (mgl-1) increased the methane production rate by about 35% and increased the power consumption of the system by about 13 times. Among the 144 cases studied, the best stirrer model is figured out as the optimal model by applying an index of performance coefficient and also this model is investigated with scale-up criteria in larger sizes. A novel equation for evaluating the power production value is suggested in real digesters.

Isolation, identification and optimization of enhanced production of laccase from Galactomyces geotrichum under solid-state fermentation.

Laccases are a group of oxidases that catalyze the oxidation of a wide range of electron rich substrates like phenolic compounds, lignin and aromatic amines. They are of interest because of their potential to be used in environmental and industrial applications. In this research, potent laccase producer fungi were screened and isolated from olive mill wastewater (OMW). One of the 23 isolated fungi was identified as Galactomyces geotrichum based on 18S rDNA sequence analysis that detected good laccase activity. Produced laccase had a molecular weight of 55 kDa that was confirmed by zymogram analysis. This is the first report about the optimization of laccase Production by G. geotrichum under solid-state fermentation. The optimization was made by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L25) was designed using Minitab 19 software to study four effective process factors in five levels for laccase production. The optimum condition derived was; moisture content (80%), fermentation time (14 day), CuSO4⋅5H2O as the inducer (300 µM), glucose as a co-substrate (5 g/L). Maximum laccase activity of 52.86 (U/g of dry substrate) was obtained using optimum fermentation condition. This study aimed to better understand the laccase producing microorganisms in OMW and take them to OMW treatment that is rich in phenolic compounds.

Application of isolated halophilic microorganisms suspended and immobilized on walnut shell as biocarrier for treatment of oilfield produced water.

Salinity expressed as total dissolved solids (TDS), is the most challenging parameter in bioremediation of produced water which may inhibit the microbial activities and cause sedimentation problems. The present study explores the feasibility of using walnut shell as an inexpensive and accessible adsorbent-carrier for the immobilization of isolated halophilic microorganisms for treatment of synthetic oilfield produced water. The moving bed biofilm reactor (MBBR) was examined with influent chemical oxygen demand (COD) concentrations from 900 to 3600 mg L-1, TDS concentrations from 35,000-200,000 mg L-1, and cycle times from 24 to 72 h. Comparison of the MBBR with the conventional sequencing batch reactor (SBR) indicated that both systems operated at lower influent COD and TDS concentrations satisfactorily; but at higher TDSs (above 150,000 mg L-1) the MBBR was more resistant to the shocks of toxicity (salinity) and organic load relative to the SBR. Also, the effluent turbidity was lower and the free sludge settling property was more favorable in the MBBR with average sludge volume index (SVI) of 38.8 mL g-1 compared to the SBR with SVI of 98.09 mL g-1. Microbial identification confirmed the presence of eight dominant halophilic species which were hydrocarbon degraders and/or denitrifiers.

ß-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design.

BACKGROUND: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme ß-galactosidase (ß-gal, ß-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. METHODS: Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was employed to screen the most significant levels of parameters, including the solid substrates (wheat straw, rice straw, and peanut pod), the carbon/nitrogen (C/N) ratios, the incubation time, and the inducer. The level of ß-gal production was measured by a photometric enzyme activity assay using the artificial substrate ortho-Nitrophenyl-ß-D-galactopyranoside. RESULTS: The results showed that C/N ratio (0.2% [w/v], incubation time (144 hour), and solid substrate (wheat straw) were the best conditions determined by the design of experiments using the Taguchi approach. CONCLUSION: Our finding showed that the use of rice straw and peanut pod, as solid-state substrates, led to 2.041-folds increase in the production of the enzyme, as compared to rice straw. In addition, the presence of an inducer did not have any significant impact on the enzyme production levels.

A novel green one-step synthesis of gold nanoparticles using crocin and their anti-cancer activities.

Functionalized nanoparticles are specifically designed to deliver drugs at tumor cells and can potentially enhance anticancer activity of drugs such as crocin. In the present study, we have applied antioxidant crocin as a reducing agent for one pot green synthesis of controlled size gold nanoparticles (AuNPs). Spherical, stable and uniform AuNPs were synthesized using crocin. These AuNPs are characterized by UV-Vis, TEM and XRD techniques. The prepared AuNPs showed surface plasm on resonance centered at 520nm with the average particle size of about 4-10nm. The anti-cancer effect of AuNPs was determined using MTT and LDH tests. The cellular data showed that these AuNPs significantly decreased cancerous cells' growth after 24 and 48hours in a time- and dose-dependent manner (P<0.05). The results suggest that such AuNPs can be synthesized simply and quickly with invaluable clinical as well as pharmaceutical activities which can help to treat human breast cancer.

Optimisation of medium composition for probiotic biomass production using response surface methodology.

This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp. lactis in apple juice and to assess the effects of these factors by using response surface methodology. A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism. A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data. It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration. Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.

In situ recovery of 2,3-butanediol from fermentation by liquid-liquid extraction.

End-product conversion, low product concentration and large volumes of fermentation broth, the requirements for large bioreactors, in addition to the high cost involved in generating the steam required to distil fermentation products from the broth largely contributed to the decline in fermentative products. These considerations have motivated the study of organic extractants as a means to remove the product during fermentation and minimize downstream recovery. The aim of this study is to assess the practical applicability of liquid-liquid extraction in 2,3-butanediol fermentations. Eighteen organic solvents were screened to determine their biocompatibility, and bioavailability for their effects on Klebsiella pneumoniae growth. Candidate solvents at first were screened in shake flasks for toxicity to K. pneumoniae. Cell density and substrate consumption were used as measures of cell toxicity. The possibility of employing oleyl alcohol as an extraction solvent to enhance end product in 2,3-butanediol fermentation was evaluated. Fermentation was carried out at an initial glucose concentration of 80 g/l. Oleyl alcohol did not inhibit the growth of the fermentative organism. 2,3-Butanediol production increased from 17.9 g/l (in conventional fermentation) to 23.01 g/l (in extractive fermentation). Applying oleyl alcohol as the extraction solvent, about 68% of the total 2,3-butanediol produced was extracted.