ABSTRACT
A pilot study evaluated the efficacy of the drug afalaza (mixture of affinity purified antibodies to PSA and endothelial NO-synthase) compared with the Serenoa repens extract in a model of chronic abacterial prostatitis in Wistar rats caused by suturing of prostate tissue by silk thread. Except for the animals of intact group, rats (n = 13 in each group) underwent intraperitoneal injection of distilled water (10 ml/kg), afalaza (at a doses of 5, 7.5 and 10 ml/kg) or an Serenoa repens extract (50 mg/kg) 1 month after surgery for 45 days. After infusion, the mass, volume, and prostate weighting factor were evaluated, and prostate tissue was examined histologically. 2.5 months after surgery, development of chronic abacterial prostatitis was observed in the control group. Compared with intact group, significant increase in weight, weighting factor, and volume of prostate were detected in control group. Against the background of administration of Serenoa repens extract and afalaza, these parameters were not significantly different from control values. The use of Serenoa repens extract prevented the development of atrophic processes and slowed the development of sclerotic processes. Administration of afalaza at all studied doses prevented the development of sclerotic changes, and a dose of 7.5 ml/kg prevented the development of atrophic processes with the effectiveness matching to Serenoa repens extract. Taking into account the high safety of afalaza, this drug is a promising treatment for chronic prostatitis.
Subject(s)
Antibodies/pharmacology , Prostatitis/drug therapy , Animals , Chronic Disease , Drug Evaluation, Preclinical , Male , Plant Extracts/pharmacology , Prostatitis/pathology , Rats , Rats, Wistar , Sclerosis/pathology , Sclerosis/prevention & control , SerenoaABSTRACT
We compared the efficacy of Impaza (antibodies against endothelial NO-synthase in ultra-low doses) and Serenoa repens on the rat model of chronic aseptic prostatic inflammation. Administration of Serenoa repens in a dose of 50 mg/kg for 1.5 months prevented the development of prostate sclerosis and increased luminal area, but did delay the development of atrophic processes. In animals treated with Impaza (3 ml/kg for 1.5 months), atrophic changes in the prostate gland were practically absent. These findings indicate that Impaza can be used in complex therapy of abacterial prostatitis.
Subject(s)
Antibodies/therapeutic use , Prostatitis/drug therapy , Animals , Erectile Dysfunction , Male , Nitric Oxide Synthase Type III/metabolism , Plant Extracts/therapeutic use , Prostatitis/metabolism , Rats , Serenoa/chemistryABSTRACT
The effects of kardostin on the ovulatory cycle, conception, and sexual behavior of female rats have been experimentally studied. It is established that a two-week therapy with kardostin at a dose of 5 and 15 mg/kg changed phases of the estrous cycle (with the estrus phase being most prevalent) and led to ambiguous changes in the sexual behavior of female rats (a dose of 5 mg/kg increased sexual behavior, while a dose of 15 mg/kg inhibited it), activated fertility, and improved the quality of conception.
Subject(s)
Angiotensin II Type 2 Receptor Blockers/pharmacology , Antibodies, Monoclonal/pharmacology , Fertility/drug effects , Fertilization/drug effects , Sexual Behavior, Animal/drug effects , Animals , Estrous Cycle/drug effects , Female , Male , Menstrual Cycle/drug effects , Nitric Oxide Synthase Type III/antagonists & inhibitors , Nitric Oxide Synthase Type III/metabolism , Pregnancy , Rats , Receptor, Angiotensin, Type 2/metabolismABSTRACT
Chronic treatment of rats with kardosten for 6 months had a positive effect on some ECG values and behavior without disordering the hepatorenal functions. All effects of the drug observed during a course of treatment were leveled and the parameters reached the basal values within 2 weeks after drug discontinuation, this confirming its safety.
Subject(s)
Antibodies/toxicity , Antihypertensive Agents/toxicity , Cardiotonic Agents/toxicity , Receptor, Angiotensin, Type 1/immunology , Animals , Antibodies/administration & dosage , Antibodies/pharmacology , Antihypertensive Agents/administration & dosage , Antihypertensive Agents/pharmacokinetics , Antihypertensive Agents/pharmacology , Biological Availability , Cardiotonic Agents/administration & dosage , Cardiotonic Agents/pharmacokinetics , Cardiotonic Agents/pharmacology , Cardiovascular System/drug effects , Dose-Response Relationship, Drug , Electrocardiography , Exploratory Behavior/drug effects , Female , Kidney/drug effects , Liver/drug effects , Male , Motor Activity/drug effects , Rats , Regional Blood Flow/drug effects , Sex FactorsABSTRACT
Tension-type headaches are the most common type of headaches in children. No effective treatment for this disease is developed yet. After treatment with tenoten, the number of days with headache in 9-17-years-old children decreased by 40.9%, the mean headache intensity score decreased to 1.
Subject(s)
Antibodies/therapeutic use , Tension-Type Headache/drug therapy , Adolescent , Antibodies/adverse effects , Child , Female , Humans , Male , Tension-Type Headache/pathology , Treatment OutcomeABSTRACT
The involvement of the serotoninergic system in the realization of anxiolytic and antidepressant activities of antibodies to S-100 protein in ultralow doses is proven. Administration of ultralow-dose antibodies to S-100 protein in combination with serotoninergic agents (ketanserin and 5-hydroxytryptophan) reduced the anxiolytic and antidepressant effects of antibodies.
Subject(s)
S100 Proteins/immunology , Serotonin/physiology , Animals , Dose-Response Relationship, Immunologic , Ketanserin/pharmacology , Male , Rats , Serotonin Antagonists/pharmacologyABSTRACT
Analysis of coherence of cortical electric activity performed in 30 healthy subjects revealed changes in the spatial organization of cortical electric activity after listening the instruction, i.e., loading the explicit working memory with a sequence of operations of the cognitive task to be solved in the course of the experiment. Comparison of instructions presenting several cognitive tasks showed that greater load of the working memory is associated with higher coherence of cortical activity, especially, in the parietotemporal and occipital areas.