Subject(s)
Syphilis Serodiagnosis/standards , Syphilis/diagnosis , Adult , Decision Trees , False Positive Reactions , Female , Hemagglutination Tests/methods , Hemagglutination Tests/standards , Humans , Latex Fixation Tests/methods , Latex Fixation Tests/standards , Male , Prospective Studies , Sensitivity and Specificity , Syphilis/classification , Syphilis/epidemiology , Tunisia/epidemiologyABSTRACT
This study was aimed at identifying the relative importance of the various agents responsible for gastroenteritis and to contribute to the etiopathogeny of this condition. Four hundred and two samples of stools of patients and 209 of control subjects, all under 3 years of age, were submitted to bacteriological, parasitologic and virologic examinations. An enteral etiology of gastroenteritis was found in 50% of the children. It consisted of enteropathogenic bacteria in 11%, rotaviruses in 29% and both associated in 19% of cases. There was a significant difference between patients and controls for bacterial (p less than 0.0001) and viral (p less than 0.00001) infestation. The most frequently isolated bacteria consisted overall of Escherichia coli. Eighty-seven isolated strains of E. Coli were tested for their enterotoxinogenic capacities. Four strains were bearing the attachment factor (EAF) and 14 were toxinogenic. Shiga-like toxin (or vero toxin) was detected in 4 strains. Campylobacter was isolated as frequently in patients as in controls and was often associated with other bacteria or rotaviruses. The presence of parasites and candidas was not significantly different in patients and in controls. Giardia intestinalis was the most frequently isolated parasite.
Subject(s)
Gastroenteritis/etiology , Acute Disease , Child, Preschool , Diarrhea, Infantile/etiology , Feces/microbiology , Feces/parasitology , Gastroenteritis/microbiology , Gastroenteritis/parasitology , Humans , Infant , Infant Food , Seasons , TunisiaABSTRACT
The incidence of Escherichia coli EPEC and ETEC had been studied on 555 infants from Tunis area. 193 of them do not present any diarrhea. Frequency of EPEC is 9.14% in infants with diarrhea and 3.10% in the group without diarrhea. Analysis of 87 Escherichia coli strains using genetic probes showed that 4 EPEC strains present effacing attachment factor (EAF) and one EPEC strain produce verotoxin. The frequency of ETEC isolated is 18.05%, the majority of them produced ST enterotoxin. Only colonization factor antigen I (CFAI) was found in ETEC strains.
Subject(s)
Diarrhea/microbiology , Escherichia coli/isolation & purification , Acute Disease , Bacterial Toxins/genetics , Child, Preschool , DNA, Bacterial/analysis , Enterotoxins/genetics , Escherichia coli/classification , Escherichia coli/genetics , Humans , Infant , Infant, Newborn , Nucleic Acid Hybridization , Serotyping , Shiga Toxin 1 , TunisiaABSTRACT
The choleriform diarrhoea may be caused by Vibrio cholerae, but also by other Vibrionaceae exhibiting the cholera-toxin antigenic determinants. The authors report three instances of gastroenteritis in infants, caused by 3 strains of non-O1 Vibrio and they carry out bacteriological study on these strains and their pathogenicity-strength factors.
Subject(s)
Diarrhea, Infantile/microbiology , Vibrio cholerae/isolation & purification , Acute Disease , Child, Preschool , Humans , Infant , Tunisia , Vibrio cholerae/classificationABSTRACT
Investigation of C. jejuni, C. coli has been realised on 280 babies in Tunis, 123 of them do not present any diarrhea. The global frequency of campylobacter isolated is 4.64%. Concerning babies with diarrhea, the frequency is 4.45%, whereas it is 4.86% in the group without diarrhea. A similar frequency of C. jejuni and C. coli appears in the two groups.
Subject(s)
Campylobacter Infections/microbiology , Diarrhea/etiology , Campylobacter/isolation & purification , Campylobacter fetus/isolation & purification , Feces/microbiology , Humans , Infant , Infant, Newborn , TunisiaABSTRACT
The antibiotics susceptibility of 480 Salmonella collected in 1982 and 1983, in the National Center of Salmonella of Pasteur Institute of Tunis was tested. High levels of resistance were found. Nalidix acid, colistin and gentamicin were the most active. Resistance and multiple resistance was most frequently found in strains of Salmonella wien and Salmonella saint paul.
Subject(s)
Anti-Bacterial Agents/pharmacology , Salmonella/drug effects , Drug Resistance, Microbial , TunisiaABSTRACT
Between 1977 and 1982, the National Center of Salmonella of the Institute Pasteur of Tunis has isolated; received and/or identified 1715 Salmonella strains. In typhoid and paratyphoid fevers group Salmonella typhi represent the predominant species with a frequency of 99.6%. In the enteric group, Salmonella wien is the most frequent (50,26%). 11 serotypes appeared in 1982, although 5 serotypes have not been met since 1977.
Subject(s)
Salmonella/isolation & purification , Enteritis/microbiology , Feces/microbiology , Humans , Paratyphoid Fever/microbiology , Salmonella/classification , Serotyping , Typhoid Fever/microbiologyABSTRACT
We report the isolation in Tunisia of two strains of Vibrio metschnik ovii from waters; These strains present some atypical characteristics the most important being their weak halophilie property.
Subject(s)
Vibrio/isolation & purification , Water MicrobiologyABSTRACT
An investigation based on 1008 pharyngeal samples effected on school children aged six to twelve years of Tunis area has shown 71 carriers of beta-hemolytic streptococci (percentage 7%) whose 42 Lancefield's group A. The strains isolated had been tested by agar diffusion technic about seven antimicrobial agents. The most active ones Ampicillin are. Tetracyclin, Oxytetracycline and Oxacillin are the less active ones.
Subject(s)
Pharynx/microbiology , Streptococcus agalactiae/isolation & purification , Urban Population , Anti-Bacterial Agents/pharmacology , Child , Drug Resistance, Microbial , Humans , Microbial Sensitivity Tests , TunisiaABSTRACT
Two hundred and one strains of Vibrio cholerae el tor were tested by agar diffusion technic against fourteen antimicrobial agents. All the strains tested are inhibited by tetracyclines, gentamycin, tobramycin, kanamycin, chloramphénicol, carbenicillin and the association of trimétomycin but mainly the colistin are the less active. A great majority of strains are inhibited by the ampicillin. The streptomycin but mainly the colistin are the less active.
