[Nitrogen transformation as a result of Pseudomonas denitrificans denitrification]. / Prevrashchenie azota v rezul'tate denitrifikatsii Pseudomonas denitrificans.

The energy process of nitrate reduction was studied in the typical soil denitrifying cultures of Pseudomonas denitrificans by mass spectrometry. These microorganisms differed in certain characteristics of the process from the cultures of sporogenic soil denitrifying bacteria belonging to the Bacillus genus. The rate of denitrification by Ps. denitrificans was very high; as the result, losses of 15N from the medium exceeded 80--90%. Ammonia was not produced as the final product of nitrate reduction in the course of denitrification. Apparently, hydroxylamine, hyponitrite or an organic intermediate product of nitrate reduction is produced in the process of its assimilation by the cultures.

[Chemcial aspects of the energy process in nitrate reduction in different representatives of soil microflora]. / Khimizm énergeticheskogo protsessa vosstanovleniia nitrata u razlichnykh predstavitelei pochvennoi mikroflory.

Chemical aspects of dissimilatory nitrate reduction were studied by mass spectrometry in the following soil bacteria: Bacillus filaris, Bacillus polymyxa and Pseudomonas denitrificans. Chemical peculiarity of this process in spore-forming soil bacteria is the simultaneous operation of two energy processes: denitrification and nitrate respiration. The first process is terminated by the formation of molecular nitrogen, the second, by the production of ammonia. The quantitative ratio between these processes demonstrates the advantage of nitrate respiration in the overall energy pathway of nitrate dissimilation. Pseudomonas denitrificans maintains the abilityfor intensive gas production as a result of denitrification upon long storage on artificial media: ca. 34 per cent of the nitrate nitrogen is reduced to gaseous forms of nitrogen.

[Chemistry of denitrification in sporogenous soil bacteria]. / Khimizm denitrifikatsii u sporonosnykh pochvennykh bakterii

Soil sporeforming denitrifying bacteria, Bacillus filaris and Bacillus polymyxa, differ by their cultural-morphological and physiological characteristics, but are similar in the chemistry of dissimilating nitrate reduction. Two processes occur simultaneously: denitrification yielding gaseous nitrogen forms, and nitrate respiration upon which nitrates are reduced to ammonia. The ratio between the two depends on physico-chemical conditions of the environment. The chemistry of dissimilating nitrate reduction by sporeforming bacteria differs therefore from nitrate transformation by other denitrifying microorganisms: Pseudomonas, Micrococcus, Rhizobium, Achromobacter, which, according to the published evidence, are capable only of denitrification.