[Production of recombinant hIL-4delta2-a native isoform of human interleukin-4 in Escherichia coli cells]. / Produktsiia rekombinantnogo hIL-4delta2-prirodnoi izoformy interleikina-4 cheloveka, v kletkakh Esherichia coli.

Expression plasmids containing the synthetic gene hil-4 delta 2 was constructed to produce human interleukin-4 in Escherichia coli cells. Strains TG1 (pBTIL-4 delta 2) and BL21 (DE3) (pETIL-4 delta 2) produced the recombinant protein as inclusion bodies, and its production level was up to 30% of the total cell protein. The renatured hIL-4 delta 2 inhibited IL-4-stimulated T cell proliferation, and this effect was enhanced by cyclosporin A.