ABSTRACT
Early defects due to the afflux of monocytic phagocytes into the inflammatory focus, rapid resorption of detritis and normalisation of correlation between contents of matrix proteinases and their inhibitors, stimulation of stromal cornea keratinocytes migration and their proliferation characterize repair of postburn defects in transplantation of the stromal equivalent. Repair against the background of stromal equivalent transplantation is of a histotypical character: collagen gel organizes and serves the basis for migration of the cornea fibroblasts and temporary matrix for growing new epithelium, while restored basal membrane is a derivative of cornea epithelium.
Subject(s)
Absorbable Implants , Corneal Stroma/immunology , Eye Burns/immunology , Regeneration/immunology , Animals , Cell Differentiation , Cell Movement/immunology , Collagen/immunology , Corneal Stroma/injuries , Corneal Stroma/pathology , Eye Burns/pathology , Eye Burns/therapy , Fibroblasts/immunology , Fibroblasts/pathology , Humans , Keratinocytes/immunology , Keratinocytes/pathology , Matrix Metalloproteinases/immunology , Phagocytes/immunology , Phagocytes/pathology , Rabbits , Rats , Rats, WistarABSTRACT
The paper contains the results of using a mew method applicable to regulation of corneal regeneration, i.e. transplantation of allogenic fibroblasts cultivated in collagen gel within an experimental model of acute alkali corneal burns in rabbits. Two experimental stages, involving 120 animals, were undertaken. The above method was used, at stage 1, on day 2 after trauma and, at stage 2, it was applied in two weeks after ocular burns with profound corneal defects were inflicted. The clinical, morphological and immune-histochemical examinations were used to show a high efficiency of the method in healing the corneal burn wounds; the mechanism of the therapeutic action of the transplant components exerted on regeneration of the affected cornea was investigated; finally, good outlooks for using the discussed method in clinical practice were proven.
Subject(s)
Burns, Chemical/surgery , Corneal Injuries , Eye Burns/chemically induced , Eye Burns/surgery , Fibroblasts/transplantation , Alkalies , Animals , Cells, Cultured , Collagen , Contact Lenses, Hydrophilic , Gels , Humans , Rabbits , Rats , Rats, Wistar , Time Factors , Transplantation, Homologous , Wound HealingSubject(s)
Biotechnology/methods , Corneal Diseases/surgery , Corneal Transplantation/methods , Biotechnology/trends , Cells, Cultured/transplantation , Corneal Transplantation/trends , Fibroblasts/cytology , Humans , Limbus Corneae/cytology , Stem Cell Transplantation/methods , Stem Cell Transplantation/trendsABSTRACT
Sluggish wounds are characterized by impaired proportions of proinflammatory cytokines, deficiency of fibrogenic growth factors, imbalance in the system of matrix metalloproteinases and their inhibitors this preventing reparation. The study was made of biopsies obtained from patients with sluggish wounds before the treatment, 5, 10 and 15 days after transplantation on the wound of allogenic EGF-stimulated cryopreserved epidermis. The wound closure with biologically active coat was followed by the reduction of expression of proinflammatory cytokines and return to their normal correlations, higher production of fibrogenic growth factors, restoration of balance in the expression of MMP-9 and TIMP-1/TIMP-2.
