[Influence of standard treatment on changes in the structural and functional properties of circulation red blood cells in obstructive and non-obstructive acute pyelonephritis].

AIM: To determine the effect of standard treatment on changes in the structural and functional properties of erythrocytes in obstructive and non-obstructive acute pyelonephritis. MATERIALS AND METHODS: The structural and functional properties of erythrocytes and their intracellular metabolism in 78 patients with a diagnosis of primary non-obstructive and secondary obstructive acute pyelonephritis, randomized by age, gender, and the minimum number of concomitant diseases were investigated. RESULTS AND DISCUSSION: In acute non-obstructive pyelonephritis, changes of the content of proteins in circulating erythrocytes responsible for the structure formation and stabilization of the plasma membrane (-spectrin, anion transport protein, pallidin, protein 4.1), intracellular metabolism (anion transport protein, glutathione-S-transferase), membrane flexibility and shape (actin, tropomyosin) are insignificant, alike from acute obstructive pyelonephritis. In addition, processes of lipid peroxidation inside red blood cells are intensified, and oxidative stress develops with a decrease in the sorption capacity of erythrocytes, as well as the content and ratio of lipid fractions in the plasma membrane, which form the basis of the lipid components and play the main role in the sequencing of protein macromolecules and the normal metabolism of red blood cells. CONCLUSION: In acute obstructive pyelonephritis, changes in the content and ratio of proteins and lipids in the erythrocyte membrane lead to functional rearrangements that are not corrected by standard treatment.

[Changes in the structural and functional properties of erythrocytes in serous and purulent acute pyelonephritis].

to establish violations of the structural and functional properties of peripheral blood erythrocytes in serous and purulent acute pyelonephritis before and after conventional therapy. MATERIALS AND METHODS: The structural and functional properties of erythrocytes of 62 patients with various forms of acute pyelonephritis, randomized by age, sex, and the minimum number of concomitant diseases in remission, were examined. Results and its discussion. In serous, to a greater extent, in the purulent form of acute pyelonephritis, violations of the usual ratio of erythrocyte membrane proteins responsible for the flexibility and shaping of the membrane, intracellular metabolism, stabilization and structure formation of the plasma membrane skeleton were established. Disturbances in the lipid content of erythrocyte membranes, which form the basis of the lipid framework of the plasma membrane and play a major role in the ordering of protein macromolecules and normal erythrocyte metabolism, were revealed. CONCLUSION: In the serous and purulent phases of inflammation, disturbances in the qualitative and quantitative variant of the protein and lipid fractions of the membranes cause functional changes in blood erythrocytes that are not normalized by standard treatment in the purulent form of the disease, which requires the development of correction methods.4 CONCLUSIONS: In patients with NDE before treatment, an increase in the level of only one representative of the investigated proteins of the circulating erythrocyte membrane out of 12, namely tropomyosin, was revealed, which can be used in the differential diagnosis of pyelonephritis forms. In patients with a purulent form of pyelonephritis, a more significant increase in lipid peroxidation processes, a weakening of the bodys antioxidant system, and a decrease in the adsorption characteristics of erythrocytes were revealed. Given the lack of effectiveness of basic treatment in relation to most indicators of the structural and functional properties of erythrocytes, it is necessary to include immunomodulatory and antioxidant drugs in the complex treatment for serous and purulent forms of acute pyelonephritis, which will help reduce complications and enhance regenerative processes. RECOMMENDATIONS: to recommend to medical specialists to use indicators of structural and functional properties of erythrocytes in difficult cases of differential diagnosis of forms of acute pyelonephritis.

[Application of laser technologies in the treatment of non-muscle-invasive bladder cancer].

This review includes the facts of bladder treatment by means of laser technologies application in historical perspective. Advantages and disadvantages of different laser technologies were considered in comparison with the traditional TUR of the bladder wall.

[Relationship between metabolic and immunological disorders in experimental acute tetrachlomethane, ischemic and alcoholic liver disease]. / Vzaimosviaz' metabolicheskikh i immunologicheskikh narushenii pri éksperimental'nom ostrom tetrakhlometanovom, ishemicheskom i alkogol'nom porazhenii pecheni.

The aim of this study was to investigate the relationship of metabolic and immunological disorders in acute tetrachlomethane, ischemic and alcoholic liver damage modelled in adult Wistar male rats weighing 120-160 g. After evaluation of metabolic and immunological parameters at the local and systemic levels, and correlation analysis was used to establish the relationship between the dynamics of the indicators against the background of experimental pathology models. The close correlation between the studied immune and metabolic parameters recognized for the tetrachlomethane, ischemic and alcoholic liver damage shows the existing "tension" between the indicators of immune and metabolic status. Such close correlation between the studied immunological and metabolic parameters at the system and local levels can serve to assess the severity of the disease, its prognosis, treatment effectiveness and preventive measures.

[Immune system disorders in acute pyelonephritis. II art].

New information about typical changes in the immune status at both local and systemic level in various forms of acute pyelonephritis is provided in the second part of the review. The literature review and analysis of current national and foreign literature dedicated to immune disorders in various forms of acute pyelonephritis has been performed. The degree and nature of changes in systemic and local immunological parameters in nonobstructive and obstructive, serous and purulent forms are highlighted in details. In particular, it has been established that in patients with obstructive and purulent pyelonephritis, there are more pronounced changes in the serum and urine level of cytokines and complement factors in comparison with serous and non-obstructive pyelonephritis. Recommendations are given on monitoring of the immunological status in patients with various forms of acute pyelonephritis in order to optimize treatment approaches. In addition, the rationales for effective pathogenetic, including specific, immunotherapy drugs for various forms of pyelonephritis are presented.

[Immune disturbances in acute pyelonephritis. Part I].

In the part I of a literature review of modern national and foreign publications dedicated to the problem of immune disorders in acute pyelonephritis, information on the role of innate and adaptive immunity in the pathogenesis of acute pyelonephritis is summarized and systematized. The relationship between the pathogens and immune system, in particular the ability of pathogens to overcome the protective immune mechanisms are discussed. The role of neutrophilic granulocytes as well as cellular immunity in the pathogenesis of acute pyelonephritis is shown. The changes in level of pro- and anti-inflammatory cytokines are separately highlighted. In conclusion, perspective directions for the studies in the area of immune disorders in acute pyelonephritis are proposed.

[Condition of the immune status at patients with the serous and purulent pyelonephritis according].

AIM: Studying of a condition of the immune status on for standard treatment at an acute serous and purulent pyelonephritis. MATERIALS AND METHODS: The immune status of 62 patients with various forms of an acute pyelonephritis, randomized on age, sex, the minimum quantity of associated diseases in a remission stage is surveyed. RESULTS: and their discussion. Purulent form of acute pyelonephritis was accompanied by higher levels of pro-inflammatory cytokines with decreased levels of anti-inflammatory cytokines. In serous form, imbalance in the cytokine system was found. Both serous and purulent forms of pyelonephritis were characterized by the imbalance in the complement system, increased production of immunoglobulins with simultaneous reduction of circulating immune complexes, decreased activity and the intensity of phagocytosis with increased metabolic activity of neutrophils. CONCLUSION: At serous and purulent forms of a pyelonephritis there are differences in an orientation and expression of changes of the immune indicators demanding development of methods of the differentiated immunorehabilitation.