ABSTRACT
An actinomycete strain was isolated from northern part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. Attempts were made to optimize the culture conditions for the production of antimicrobial metabolites by this strain. Antimicrobial metabolites production was started after 7 days of incubation of culture broth and reached its maximum levels after 10 days and thereafter gradually decreased. The maximum production of antimicrobial metabolites was obtained when the culture medium pH was adjusted to 8. The optimum temperature for antimicrobial metabolites production was 39â, indicated the new strain as mesophilic organism. Basel medium supplemented with glucose and yeast extract as carbon and nitrogen sources, respectively, was proved to be the best for the production of bioactive metabolites. Maximum production of bioactive metabolites was when NaCl concentration was 1% and among different minerals tested, K2HPO4 and NaCl showed positive influence on antibiotic production by the strain.
ABSTRACT
Se analizaron extractos crudos y un ácido graso, ácido octadec-7-en-5-ynoic (1), de la corteza de la raíz de Cappariszeylanica Linn. (familia de las Capparidaceae) para observar sus actividades antibacterianas frente a la bacteria Gram positiva y Gram negativa. Entre los extractos crudos, el extracto de cloroformo mostró una buena actividad frente a todos los organismos de prueba. El ácido graso (1) aislado del extracto de cloroformo mostró actividades antibacterianas frente a todos los organismos de prueba, a excepción de E. coli. Las actividades se compararon con un antibiótico estándar: la kanamicina. Las concentraciones inhibitorias mínimas (CIH) de 1, determinadas mediante la técnica de dilución en serie, fueron 64 mg/ml frente a Bacillus subtilis y Shigella dysenteriae. Las actividades citotóxicas del extracto crudo y del ácido graso (1) se observaron mediante el bioensayo de gambas en salmuera yel valor de LC50 del compuesto fue 6,27 mg/ml (AU)
Crude extracts and a fatty acid, octadec-7-en-5-ynoic acid (1), from the root bark of Capparis zeylanica Linn. (Fam. Capparidaceae) were screened for their antibacterial activities against Gram positive and Gram negative bacteria. Among the crude extracts, chloroform extract showed good activity against all test organisms. The fatty acid (1) isolated from chloroform extract exhibited antibacterial activities against test organisms except E. coli. The activities were compared to a standard antibiotic- kanamycin. The minimum inhibitory concentrations (MICs) of 1, determined byserial dilution technique, were found to be 64 mg/ml against Bacillus subtilis and Shigella dysenteriae. The cytotoxic activities of crude extract and fatty acid (1) were observed by brine shrimp biassay and LC50 value of the compound was found to be 6.27 mg/ml (AU)
Subject(s)
Capparis , Capparis/microbiology , Capparis/toxicity , Plants, Medicinal/chemistry , Cytotoxicity, Immunologic , Herbicides/chemistry , Herbicides/pharmacology , Herbicides/toxicity , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/pharmacokinetics , Anti-Bacterial Agents/toxicity , Antibodies, Bacterial/pharmacology , Herbicides/metabolism , Herbicides/pharmacokinetics , Herbicides/therapeutic use , Cholera/therapy , Communicable Diseases/drug therapyABSTRACT
(+)-Catechin, 3,4-dimethoxycinnamyl alcohol and 3,4,5-trimethoxycinnamyl alcohol were isolated from the barks of Loranthus globosus. All compounds showed significant antimicrobial and cytotoxic activities.
