Starch-Based Polymer Materials as Advanced Adsorbents for Sustainable Water Treatment: Current Status, Challenges, and Future Perspectives.

Over the past three decades, chemical and biological water contamination has become a major concern, particularly in the industrialized world. Heavy metals, aromatic compounds, and dyes are among the harmful substances that contribute to water pollution, which jeopardies the human health. For this reason, it is of the utmost importance to locate methods for the cleanup of wastewater that are not genuinely effective. Owing to its non-toxicity, biodegradability, and biocompatibility, starch is a naturally occurring polysaccharide that scientists are looking into as a possible environmentally friendly material for sustainable water remediation. Starch could exhibit significant adsorption capabilities towards pollutants with the substitution of amide, amino, carboxyl, and other functional groups for hydroxyl groups. Starch derivatives may effectively remove contaminants such as oil, organic solvents, pesticides, heavy metals, dyes, and pharmaceutical pollutants by employing adsorption techniques at a rate greater than 90%. The maximal adsorption capacities of starch-based adsorbents for oil and organic solvents, pesticides, heavy metal ions, dyes, and pharmaceuticals are 13,000, 66, 2000, 25,000, and 782 mg/g, respectively. Although starch-based adsorbents have demonstrated a promising future for environmental wastewater treatment, additional research is required to optimize the technique before the starch-based adsorbent can be used in large-scale in situ wastewater treatment.

Characterisation of Polypropylene Composite Reinforced with Chemi-Thermomechanical Pulp from Oil Palm Trunk via Injection Moulding Process.

As the products made from wood-plastic composites (WPCs) become more sophisticated and required more detail profiles, the injection moulding processing method with wood pulp as the reinforcing material is the answer to cater to the rapid change and demands of composite products. The general objective of this study was to study the effects of the material formulation, as well as the injection moulding process parameters, on the properties of a polypropylene composite reinforced with chemi-thermomechanical pulp from oil palm trunks (PP/OPTP composite) via the injection moulding process. The PP/OPTP composite with a material formulation of 70% pulp/26% PP/4% Exxelor PO produced using injection moulding at 80 °C as the mould temperature and with 50 tonnes of injection pressure exhibited the highest physical and mechanical properties. The increment loading of pulp increased the water absorption capacity of the composite. Higher loading of the coupling agent effectively reduced the water absorption capacity and increased the flexural strength of the composite. The increase in mould temperature from unheated to 80 °C prevented excessive heat loss of the flowing material, which enabled the molten material to flow better and filled up all cavities in the mould. The increased injection pressure slightly improved the physical properties of the composite, but the effect on the mechanical properties was insignificant. For the future development of WPCs, further studies should be focused on the viscosity behaviour, as a greater understanding of the processing parameters' effects on the PP/OPTP's viscosity behaviour will lead to improved product design and enable great potential usage of WPCs.

Exploring the Biocontrol Efficacy of Trichoderma spp. against Rigidoporus microporus, the Causal Agent of White Root Rot Disease in Rubber Trees (Hevea brasiliensis).

Rigidoporus microporus, which causes white root rot disease (WRD) in Hevea brasiliensis, is a looming threat to rubber plantation in Malaysia. The current study was conducted to determine and evaluate the efficiency of fungal antagonists (Ascomycota) against R. microporus in rubber trees under laboratory and nursery conditions. A total of 35 fungal isolates established from the rubber tree rhizosphere soil were assessed for their antagonism against R. microporus by the dual culture technique. Trichoderma isolates can inhibit the radial growth of R. microporus by 75% or more in the dual culture test. Strains of T. asperellum, T. koningiopsis, T. spirale, and T. reesei were selected to assess the metabolites involved in their antifungal activity. Results indicated that T. asperellum exhibited an inhibitory effect against R. microporus in both volatile and non-volatile metabolite tests. All Trichoderma isolates were then tested for their ability in producing hydrolytic enzymes such as chitinase, cellulase and glucanase, indole acetic acid (IAA), siderophores production, and phosphate solubilization. From the positive results of the biochemical assays, T. asperellum and T. spirale were selected as the biocontrol candidates to be further tested in vivo against R. microporus. The nursery assessments revealed that rubber tree clone RRIM600 pretreated with only T. asperellum or with the combination of T. asperellum and T. spirale was able to reduce the disease severity index (DSI) and exert higher suppression of R. microporus compared to other pretreated samples, with the average DSI below 30%. Collectively, the present study demonstrates that T. asperellum represents a potential biocontrol agent that should be further explored to control R. microporus infection on rubber trees.

Valorisation of Underutilized Grass Fibre (Stem) as a Potential Material for Paper Production.

An integrated and feasible approach was proposed using the underutilized grass fibre (stem) derived from Napier grass and sugarcane for paper production in this study. To enhance paper strength, pre-hydrolysis and beating techniques have been used to improve the chemical pulps and mechanical pulping process, respectively. Napier grass and sugarcane are promising non-wood sources for pulp production, owing to their high cellulose and low lignin and extractive content. With the additional mild alkaline pre-treatment to the mechanical pulping process, the lignin content was greatly reduced. The results reveal that the mechanical pulping with alkaline pre-treatment may indeed potentially replace the most prevalent pulping process (chemical pulping). As evidenced by the paper strength properties, mechanical pulping is far more suitable for grass-type biomass, particularly Napier grass, which had a folding endurance capability five times greater than chemical pulping. Furthermore, the remaining high hemicellulose content from mechanical pulping contributed to a high pulp yield, while also facilitating the fibrillation on the sugarcane's laboratory paper handsheet. The findings also demonstrated that the additional beating process from chemical pulping causes the fibres to be drawn toward each other, resulting in a more robust fibre network that contributes to good paper strength. Consequently, this work sheds new light on the development of advanced paper derived from grass fibre.

Production and Potential Application of Pyroligneous Acids from Rubberwood and Oil Palm Trunk as Wood Preservatives through Vacuum-Pressure Impregnation Treatment.

The development of low-environmental-impact technologies for the elimination of biological damage is one of the vital goals of the wood protection industry. The possibility of utilizing pyroligneous acid as a wood preservative can be a great solution to extend the application of the currently fast-growing timber species, which has lower natural durability against biological damage. In this study, the effectiveness of pyroligneous acid as a wood preservative was evaluated by impregnating rubberwood with pyroligneous acid using vacuum-pressure treatment, and the treated woods were exposed to mould fungi, wood-decay fungi and termite attacks under laboratory conditions. Pyroligneous acids produced from rubberwood (RWPA) and oil palm trunk (OPTPA) at different pyrolysis temperatures were evaluated. To fully understand the effectiveness of pyroligneous acids as wood preservatives, different concentrations of pyroligneous acids were impregnated into rubberwood. Concentrations of 50% RWPA and 30% OPTPA were sufficient against mould and decay fungi on rubberwood. Rubberwood impregnated with pyroligneous acid acted as a slow-acting toxic bait to cause a high termite mortality rate due to toxic feeding and does not serve as a good repellent to prevent termites from feeding on the wood. In general, OPTPA has better biological durability compared to RWPA.

Fabrication of Highly Microporous Structure Activated Carbon via Surface Modification with Sodium Hydroxide.

The aim of this study was to select the optimal conditions for the carbonization process followed by surface modification treatment with sodium hydroxide (NaOH) to obtain a highly microporous activated carbon structure derived from palm kernel shells (PKS) and coconut shells (CS). The effects of the carbonization temperature and NaOH concentration on the physiochemical properties, adsorption capability, specific surface area, surface morphology, and surface chemistry of PKS and CS were evaluated in this study. The results show that surface-modified activated carbons presented higher surface area values (CS: 356.87 m2 g-1, PKS: 427.64 m2 g-1), smaller pore size (CS: 2.24 nm, PKS: 1.99 nm), and larger pore volume (CS: 0.34 cm3 g-1, PKS: 0.30 cm3 g-1) than the untreated activated carbon, demonstrating that the NaOH surface modification was efficient enough to improve the surface characteristics of the activated carbon. Moreover, surface modification via 25% NaOH greatly increases the active functional group of activated carbon, thereby directly increasing the adsorption capability of activated carbon (CS: 527.44 mg g-1, PKS: 627.03 mg g-1). By applying the NaOH post-treatment as the ultimate surface modification technique to the activated carbon derived from PKS and CS, a highly microporous structure was produced.

Effect of Glue Spreads on the Structural Properties of Laminated Veneer Lumber from Spindleless Rotary Veneers Recovered from Short Rotation Hevea Plantation Logs.

Unproductive young rubber trees (15 years old) with smaller diameters (15 to 18 cm) compared to conventional rubber logs, harvested at the age of 25 years old, were selected for the production of laminated panels. Spindleless rotary veneer peeling was applied to logs from short-rotation rubber forest plantations to produce veneers for structural purposes. This raises questions about the utilization of these small-diameter logs with respect to its effect on the quality of veneer and laminated panels produced. This study examines the effect of the glue spread rates on the physical and mechanical properties of rubberwood laminated veneer lumber (LVL). Analysis of variance shows that the application of a 280 g/m2 glue spread rate significantly improved the density, water absorption and dimensional stability of rubberwood LVL. The mechanical properties of rubberwood LVL produced with a 200 g/m2 glue spread rate met the minimum requirement for the 2.1E-3100F stress class; 91.05 MPa for the modulus of rupture in the flatwise direction and 50.23 MPa for compressive strength parallel to the longitudinal axis. The modulus of elasticity in the flatwise direction of 11,189.55 MPa reached the minimum requirement for the 1.5E-2250F stress class.

Virulence of Rigidoporus microporus Isolates Causing White Root Rot Disease on Rubber Trees (Hevea brasiliensis) in Malaysia.

Latex production from Hevea brasiliensis rubber tree is the second most important commodity in Malaysia, but this industry is threatened by the white root rot disease (WRD) caused by Rigidoporus microporus that leads to considerable latex yield loss and tree death. This study aimed to characterize and compare the virulence of five R. microporus isolates obtained from infected rubber trees located at different states in Malaysia. These isolates were subjected to morphological and molecular characterization for species confirmation and pathogenicity test for the determination of virulence level. BLAST search showed that the ITS sequences of all the pathogen isolates were 99% identical to R. microporus isolate SEG (accession number: MG199553) from Malaysia. The pathogenicity test of R. microporus isolates conducted in a nursery with 24 seedlings per isolate showed that isolate RL21 from Sarawak has developed the most severe above- and below-ground symptoms of WRD on the rubber clone RRIM600 as host. Six months after being infected with R. microporus, RL21 was evaluated with the highest average of disease severity index of 80.52% for above- and below-ground symptoms, followed by RL22 (68.65%), RL20 (66.04%), RL26 (54.38%), and RL25 (43.13%). The in vitro growth condition tests showed that isolate RL21 of R. microporus has optimum growth at 25-30 °C, with the preference of weakly acidic to neutral environments (pH 6-7). This study revealed that different virulence levels are possessed among different R. microporus isolates even though they were isolated from the same host species under the same climate region. Taken together, field evaluation through visual observation and laboratory assays have led to screening of the most virulent isolate. Determination of the most virulent isolate in the present study is vital and shall be taken into consideration for the selection of suitable pathogen isolate in the development of more effective control measures in combating tenacious R. microporus.

Application strategies by selective medium treated with entomopathogenic bacteria Serratia marcescens and Pseudomonas aeruginosa as potential biocontrol against Coptotermes curvignathus.

The success of microbial termiticides in controlling termites depends on the ability of microbes to grow in different media and the functionality of the microbes as a resistant barrier or toxic bait. This study was conducted to understand the mortality rate and behaviour changes of the subterranean termite Coptotermes curvignathus Holmgren introduced with different concentrations of Serratia marcescens strain LGMS 1 and Pseudomonas aeruginosa strain LGMS 3 using wood and soil as bacterial transfer medium. In general, higher concentration of bacteria in soil caused a reduction in tunnelling activity and wood consumption and an increase in mortality. However, application on wood revealed a different outcome. Wood treated with S. marcescens of 106 CFU ml-1 concentration proved to be more efficient as bait than higher concentration applications as it caused a high mortality rate while still highly palatable for termites. Wood or soil treated with S. marcescens concentration higher than 109 CFU ml-1 creates a high toxicity and repellent barrier for termites. Pseudomonas aeruginosa of 109 CFU ml-1 concentrations applied on wood served as a slow-acting toxic bait. However, the ability for S. marcescens and P. aeruginosa to survive on wood is low, which made the bait unable to retain a useful level of toxicity for a long period of time and frequent reapplication is needed.

Septicaemia of subterranean termites Coptotermes curvignathus caused by disturbance of bacteria isolated from termite gut and its foraging pathways.

Microbial pathogens continue to attract a great deal of attention to manage the termite population. Every bacterium has its own mode of action and in fact, the mechanisms used by bacteria to attack termites remain elusive at the moment. Hence, the objective of this study was to evaluate the susceptibility of subterranean termites Coptotermes curvignathus to opportunistic pathogens using culturable aerobic bacteria isolated from the termite gut and its foraging pathways. Bacterial suspensions were prepared in concentrations of 103, 106 and 109 colony-forming units (CFU) ml-1 and introduced to the termites via oral-contact and physical contact treatment. The data show that contact method acted slower and gave lower mortality, compared to the oral-contact method. Coptotermes curvignathus were highly susceptible to Serratia marcescens and Pseudomonas aeruginosa. Serratia marcescens showed the highest mortality percentage of 68% and 54% at bacterial concentration of 109 CFU ml-1 via oral-contact and contact method, respectively. Serratia marcescens was also defined as the bacteria with the highest ability to induce the high mortality of C. curvignathus with the lowest concentration of bacterial suspension at a given time under laboratory condition. The results of this study indicate that P. aeruginosa and S. marcescens in particular may be attractive candidates worth further examination as a possible biocontrol agent against C. curvignathus in the field and to evaluate environmental and ecological risks of the biocontrol.

Production of bioadsorbent from phosphoric acid pretreated palm kernel shell and coconut shell by two-stage continuous physical activation via N2 and air.

In the present study, agricultural biomass-palm kernel shell (PKS) and coconut shell (CS)-was used to produce high porosity bioadsorbent using two-stage continuous physical activation method with different gas carrier (air and N2) in each stage. The activation temperature was set constant at 600, 700, 800 or 900°C for both activation stages with the heating rate of 3°C min-1. Two parameters, the gas carrier and activation temperature, were determined as the significant factors on the adsorption properties of bioadsorbent. BET, SEM, FTIR, TGA, CHNS/O and ash content were used to elucidate the developed bioadsorbent prepared from PKS and CS and its capacity towards the adsorption of methylene blue and iodine. The novel process of two-stage continuous physical activation method was able to expose mesopores and micropores that were previously covered/clogged in nature, and simultaneously create new pores. The synthesized bioadsorbents showed that the surface area (PKS: 456.47 m2 g-1, CS: 479.17 m2 g-1), pore size (PKS: 0.63 nm, CS: 0.62 nm) and pore volume (PKS: 0.13 cm3 g-1, CS: 0.15 cm3 g-1) were significantly higher than that of non-treated bioadsorbent. The surface morphology of the raw materials and synthesized bioadsorbent were accessed by SEM. Furthermore, the novel process meets the recent industrial adsorbent requirements such as low activation temperature, high fixed carbon content, high yield, high adsorption properties and high surface area, which are the key factors for large-scale production of bioadsorbent and its usage.