ABSTRACT
Antitumor efficiency of fascaplysin synthetic derivatives (7-phenylfascaplysin, 3-chlorofascaplysin, 3-bromofascaplysin, and 10-bromofascaplysin) was compared out in vitro on C6 glioma cells. The cytotoxic efficiency of all tested compounds was higher than that of unsubstituted fascaplysin; 3-bromofascaplysin and 7-phenylfascaplysin exhibited the best capacity to kill glioma C6 cells. Apoptosis was the main mechanism of glioma cell death. The cytotoxic activity of these compounds increased with prolongation of exposure to the substance and increase of its concentration. Fascaplysin derivatives modified all phases of glioma cell vital cycle. The count of viable tumor cell in G0 phase remained minimum by the end of experiment under the effects of 3-bromofascaplysin and 7-phenylfascaplysin.
Subject(s)
Glioblastoma/metabolism , Indoles/pharmacology , Animals , Apoptosis/drug effects , Cell Cycle/drug effects , Cell Death/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Glioma/metabolismABSTRACT
The mechanisms of therapeutic resistance of human glioblastoma multiforme are analyzed. The authors make an attempt at systematization and scientific theoretical validation of new approaches to creation of biomedical cellular preparations, based on the oncoproteomic technologies, for personified therapy of the glial tumors. A new approach to the treatment of glioblastoma multiforme with due consideration for the molecular biological characteristics of the tumor stem cells is suggested. It is shown that the tumor stem cell proteome can be regarded as the main target for cell therapy.
Subject(s)
Glioblastoma/metabolism , Glioblastoma/therapy , Genomics , Humans , Neoplastic Stem Cells/metabolism , Proteomics , Transcriptome/geneticsABSTRACT
The effects of nonstarch polysaccharides with different molecular weights on the development of Lewis' lung carcinoma and efficiency of cyclophosphamide therapy in mice were studied. Treatment with these substances with low molecular weights (<30 kDa) caused no changes in the primary tumor growth, but inhibited its metastasizing, while nonstarch polysaccharides with high molecular weights (>400 kDa) inhibited the growth of Lewis' lung carcinoma node. Antimetastatic effects of cyclophosphamide were stimulated by low and high molecular weight polysaccharides.
Subject(s)
Carcinoma, Lewis Lung/drug therapy , Cyclophosphamide/therapeutic use , Polysaccharides/therapeutic use , Animals , Carcinoma, Lewis Lung/pathology , Drug Synergism , Female , Mice , Mice, Inbred C57BL , Molecular WeightABSTRACT
The effects of various alginate forms on the development of transplanted tumors in mice and efficiency of cytostatic therapy were studied. High-molecular-weight Ca and Na alginates, acid-soluble hydrolysate, and sodium alginate fraction inhibited the growth of Ehrlich adenocarcinoma. The use of acid-insoluble sodium alginate in chemotherapy protocol improved the treatment efficiency. All alginate forms inhibited metastasizing of Lewis pulmonary carcinoma; in combination with cyclophosphamide they potentiated its antimetastatic effect.
Subject(s)
Alginates/therapeutic use , Animals , Carcinoma, Ehrlich Tumor/drug therapy , Carcinoma, Lewis Lung/drug therapy , Female , Glucuronic Acid/therapeutic use , Hexuronic Acids/therapeutic use , Male , Neoplasm TransplantationABSTRACT
In vitro experiments showed that calcium pectate added to the culture medium produces a dose-dependent prebiotic effect on lacto-and bifidobacteria cultures and on non-pathogenic strain of Escherichia coli. Calcium pectate produced a pronounced bacteriostatic effect on Candida albicans strain; the effects was more pronounced in a concentration of 4%.
Subject(s)
Pectins/pharmacology , Polysaccharides/pharmacology , Anti-Infective Agents/pharmacology , Bifidobacterium/drug effects , Candida albicans/drug effects , Escherichia coli/drug effectsABSTRACT
Experiments on mice and rats with modeled neurogenic and indomethacin-induced injuries to the gastric mucosa showed that preventive courses of calcium alginate, calcium pectate, and low-esterified pectin improved the resistance of the gastroduodenal mucosa to the destructive effect of the above factors.
Subject(s)
Gastric Mucosa/drug effects , Polysaccharides/pharmacology , Protective Agents/pharmacology , Animals , Female , Gastric Mucosa/pathology , Male , Rats , Restraint, Physical , Sex Characteristics , Stress, PsychologicalABSTRACT
We studied the effects of low-esterified pectin, calcium pectate, and alginate on the development of transplanted Ehrlich adenocarcinoma and Lewis pulmonary carcinoma and on the efficiency of cyclophosphamide treatment in mice.
Subject(s)
Antineoplastic Agents/therapeutic use , Carcinoma, Ehrlich Tumor/drug therapy , Carcinoma, Lewis Lung/drug therapy , Polysaccharides/chemistry , Polysaccharides/therapeutic use , Animals , Animals, Outbred Strains , Carcinoma, Ehrlich Tumor/pathology , Carcinoma, Lewis Lung/pathology , Cyclophosphamide/therapeutic use , Dose-Response Relationship, Drug , Female , Immunosuppressive Agents/therapeutic use , Mice , Mice, Inbred C57BL , Neoplasm Metastasis , Neoplasm Transplantation , Tumor Burden/drug effectsABSTRACT
We studied the effects of low-esterified pectin and antistrumin in rats with hypofunction of the thyroid gland modeled by enteral administration of lead acetate. Pectin more rapidly recovered thyroid function than iodine.
