[Morphological characteristics of kidneys connective tissue of mature fetuses and newborns from mothers, whose pregnancy was complicated by preeclampsia of varying degrees of severity].

OBJECTIVE: Introduction: The kidneys connective tissue condition in the antenatal period affects the formation of tissues and it changes with the development of various general pathological processes in this organ. The aim of the study was to identify the morphological features of kidneys connective tissue of fetuses and newborns from mothers whose pregnancy was complicated by preeclampsia of varying degrees of severity. PATIENTS AND METHODS: Materials and methods: The material of the study was the tissue of kidneys of mature fetuses and newborns from mothers with physiological pregnancy (28 cases), as well as from mothers whose pregnancy was complicated by preeclampsia of varying degrees of severity (78 cases). Immunohistochemical study was performed by an indirect Coons method according to M. Brosman's technique using monoclonal antibodies to collagen type I, III and IV. RESULTS: Results: The kidneys connective tissue of fetuses and newborns developing under the maternal preeclampsia conditions is characterized by the qualitative and quantitative changes that indicate the development of sclerotic processes in this organ, the severity of which increase with the age and with the increase of the maternal preeclampsia severity. Qualitative changes are characterized by an increase of the fibrous component, thickening of the bundles of connective tissue fibers, and a decrease in the distance between them. Quantitative changes are characterized by a pronounced predominance of collagen fibers over elastic fibers, almost total absence in some field of view elastic fibers and the violation of the content of collagen type I, III and IV. CONCLUSION: Conclusion: Maternal preeclampsia underlies the development of qualitative and quantitative changes in kidneys connective tissue of fetuses and newborns, which as a result will lead to disruption of the functions of these organs in such children.

[Effect of experimental acute postnatal and mixed hypoxia on the morphological state of the rats liver stromal component].

OBJECTIVE: Introduction: In children population of Ukraine the diseases of the gastrointestinal tract are quite common, among which a significant number of cases occur in liver diseases. The complexity of the liver functions formation in antenatal and intranatal periods, its morphofunctional immaturity lead to the high sensitivity of this organ to the influence of various factors from the mother organism. Actual issues are the detection of the mother's pathology influence on the formation of diseases «cargo¼ in children remote stages of ontogenesis. In literature there are no data about the influence of hypoxia as the most common damaging factor in antenatal, intranatal and postnatal periods on the liver morphofunctional state of children of different ages, including its stromal component. The aim of the study was the detection of morphological features of the stromal component of the rats liver, which were in different terms of postnatal ontogenesis, exposed to acute postnatal and mixed hypoxia. PATIENTS AND METHODS: Materials and methods: The study material was the rats liver tissue. In this study three groups were formed: I, which included rats of WAG line, born to mothers with physiological pregnancy and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis; II, which included rats of WAG line, born to mothers with physiological pregnancy, who were exposed to high altitude hypoxia on the 1st day of postnatal ontogenesis and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis; III, which included rats of «Black hood¼ line, that developed under conditions of chronic intrauterine hypoxia due to the presence of arterial hypertension in their mothers, exposed to high altitude hypoxia on the 1st day of postnatal ontogenesis and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis. It was conducted the morphometrical study for determine the specific volume of the liver stroma and an immunohistochemical study using monoclonal antibodies to types I, III collagen and fibronectin. RESULTS: Results: Mixed experimental hypoxia leads to increase of the specific volume of liver stroma of descendants from the 1st to the 35th days of postnatal ontogenesis due to the sclerotic changes development, which manifest by thickening of the connective tissue fibers bundles, a decrease the distance between them, the presence of places among the connective tissue fibers with only collagen fibers, activation the collagen formation processes with the predominance of the mature type I collagen over the immature type III collagen, increase fibronectin expression. Acute postnatal hypoxia does not affect the specific volume of the liver stroma of descendants from the 1st to the 35th days, however since the 14th day leads to the sclerotic changes development in this organ, which increase to the 35th day, less pronounced compared with the detected sclerotic changes in thecase of mixed hypoxia, and are characterized by the thickening of the connective tissue fibers bundles with the subsequent decrease the distance between them, the expressed predominance of collagen fibers over elastic fibers, increased fibronectin expression, activation the collagen formation processes with the predominance of the type I collagen over the type III collagen. CONCLUSION: Conclusions: Acute postnatal hypoxia from the 14th day of postnatal life and mixed hypoxia the 1st day lead to the sclerotic changes development in the children liver, which are more pronounced in cases of mixed hypoxia modeling and increase with the age.