ABSTRACT
Due to the steady increase in the number of patients with diabetes mellitus, as well as the development of severe complications of this pathology, the search and development of safe methods of treatment and prevention of the disease is relevant. In this direction, it seems appropriate to study the therapeutic and prophylactic properties of blueberry paste made using a unique THD-technology of the company Liqberry®. The purpose - experimental study of the anti-inflammatory properties of high-dose blueberry paste Liqberry® in a model of type 2 diabetes mellitus in laboratory Wistar rats. The experiment was conducted on 30 female Wistar rats in a model of type 2 diabetes mellitus (DM 2), which was induced by subcutaneous administration of protamine sulfate twice daily 1mg/kg for 15 days. Rats were divided into four groups: 1, intact (n=6); 2, model DM 2 (n=8); 3, DM 2 + 8 g/kg paste (n=8); and 4, DM 2 + 20 g/kg paste (n=8). The rats received Liqberry® blueberry paste daily in the morning one week after the start of the DM 2 simulation. The rats were killed on day 16 under thiopental anesthesia (20 mg/kg). Hemoglobin, erythrocytes, leukocytes, and leukocyte counts were determined in peripheral blood, and glucose levels and elastase activity were determined in serum. Simulation of DM 2 caused an increase in the blood glucose level and elastase activity, as well as in the level of stabular and segmented neutrophils by 40.6% against the background of a 14.5% decrease in the leucocyte count, 16.7% decrease in the monocyte count and 11.3% decrease in lymphocyte count, which indicates development of inflammation and decreased immunological reactivity in animals with DM 2. Administration of blueberry paste to rats with DM 2 effectively reduced blood glucose levels: a dose of 8 g/kg - by 15.9%, and a dose of 20 g/kg - by 21.2%. Also the 20 g/kg dose of the paste resulted in normalization of serum elastase activity and neutrophil counts in the blood of rats with DM 2. Blueberry paste in two doses increased the number of monocytes to normal levels and had no effect on the number of lymphocytes in the blood of animals with DM 2. The results of the study indicate a pronounced ability of bilberry paste to reduce glucose levels, inhibit the development of inflammation and prevent a decrease in immunological reactivity induced by the modeling of DM 2. The high dose of blueberry paste (20 g/kg) Liqberry® had a definite advantage.
