ABSTRACT
The influence of metabolites of arachidonic acids on the electrophoretic mobility of the rat erythrocytes has been investigated. It is found that they can increase or decrease the surface charge of the erythrocyte membranes.
Subject(s)
Arachidonic Acids/pharmacology , Erythrocyte Membrane/drug effects , Hydroxyeicosatetraenoic Acids/pharmacology , Animals , Arachidonic Acid , Cell Movement/drug effects , Cell Movement/physiology , Culture Media , Electric Conductivity/drug effects , Electric Conductivity/physiology , Electrophoresis , Erythrocyte Membrane/physiology , In Vitro Techniques , Male , RatsABSTRACT
The arachidonic acid metabolites, lipoxin B (5,14,15-trihydroxy-6,8,10,12-eicosatetraenoate) and 5,15-dihydroxy-eicosatetraenoate were shown to possess a marked effect on platelet aggregation and procoagulant activity. Lipoxin B increases and 5,15-dihydroxy-eicosatetraenoate decreases spontaneous platelet aggregation and procoagulant activity when added at various concentrations. The drugs tested were found to have no direct action on blood coagulation.
Subject(s)
Hydroxyeicosatetraenoic Acids/pharmacology , Lipoxins , Platelet Aggregation/drug effects , Animals , Arachidonic Acid , Arachidonic Acids/pharmacology , Blood Coagulation/drug effects , Hydroxyeicosatetraenoic Acids/administration & dosage , Male , Rats , Rats, Inbred StrainsABSTRACT
The lipoxin B (5, 14, 15-trihydroxy-6, 8, 10, 12-eicosatetraenoate) obtained by the enzymatic peroxidation of 5, 15-dihydroxy-6, 8, 11, 13-eicosatetraenoate (5, 15-DHETE) with the homogenous rabbit reticulocytes lipoxygenase and purified by HPLC stimulates the proliferation and differentiation of granulocyte-monocyte colony formation units (CFU-GMdc) of human peripheral blood at 3.6 X 10(-9) 3.6 X 10(-8)M in diffusion chamber placed in mouse abdominal cavity. The lipoxin B precursors namely 5,15-DHETE and arachidonic acid had stimulating influence at about 10(-6) and 10(-3)M respectively. The formation of polynuclear cells under the action of the studied lipoxygenase metabolites of the arachidonic acid was shown.
