Seasonal variations of DNA-synthesis in intestinal epithelial cells of hibernating animals--2. DNA-synthesis in intestinal epithelial cells of ground squirrel (Citellus undulatus) during autumn and late hibernation season.

1. DNA-synthesis was found to be gradually reduced in the intestinal crypt cells of ground squirrel during autumn at the time of preparation for hibernation and gradually rose during late hibernation season in February-March at the time of preparation for arousal. 2. A conclusion is made on the seasonal variations in cell replacement of true hibernators.

[Effect of extracorporeal sorption on the content of free intracellular calcium of the leukocytes in atopic bronchial asthma]. / Issledovanie vliianiia ékstrakorporal'noi sorbtsii na soderzhanie svobodnogo vnutrikletochnogo kal'tsiia v leikotsitakh pri atopicheskoi bronkhial'noi astme.

Antigen-IgE-antibody interaction on the cell surface in an allergic reaction increases methylation of phospholipids of a cell membrane making it permeable for Ca2+ ions. Therefore of interest is a study of free intracellular Ca2+ concentration from a view-point of development of atopic bronchial asthma and the use of extracorporal methods of its therapy permitting the elimination of IgE-antibodies from the blood flow. The level of intracellular Ca2+ in the leukocytes of patients with atopic bronchial asthma was studied. It has been shown that 1. the fraction of leukocytes with a higher level of intracellular Ca2+ in patients with atopic bronchial asthma was increased as compared to healthy donors; 2. extracorporal sorption results in a decrease in the number of leukocytes with high Ca concentration.

The intestinal epithelial cells of ground squirrel (Citellus undulatus) accumulate at G2 phase of the cell cycle throughout a bout of hibernation.

1. The mitotic index was found to be greatly reduced in the intestinal crypt cells of ground squirrel during bout of hibernation. The percentage of mitosis rose abruptly at least 2 hr after arousal. 2. An increase in the number of G2 cells was found in the intestinal tract of ground squirrel during bouts of hibernation. 3. The conclusion can be drawn that the cells are progressing steadily through the cell cycle. The cells accumulate at G2 in hibernation. 4. It was assumed that the block in G2 prevents the cells from possible damage in mitosis under hypothermia accompanying hibernation and, therefore, it represents an adaptive reaction.